Development of an auxiliary liver using a small intestinal segment packed together with liver micro fragments and stem-like cells of the liver

• Project/Area Number: 25670568
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Gunma University
• Principal Investigator: Kogure Kimitaka 群馬大学, 生体調節研究所, 研究員 (20125850)
• Co-Investigator(Kenkyū-buntansha): Kojima Itaru 群馬大学, 生体調節研究所, 教授 (60143492)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 有茎分節腸管 / 肝組織片移植 / 肝幹様細胞移植 / 肝幹様細胞の分化誘導 / 体内型補助肝臓 / 肝移植 / 肝不全 / 肝組織移植 / 有茎腸管グラフト / 肝幹様細胞 / 肝組織幹様細胞共充填 / 体内型補助人工肝臓 / 肝組織片充填 / 肝幹様細胞共充填

Outline of Final Research Achievements

This study was conducted to develop an auxiliary liver using a small intestinal segment packed with the minced liver and the stem-like cells of another rat liver by exposing them under the rapid growth environment of the minced liver to expect the transformation of them to the functioning hepatic cells. Hepatic stem-like cells co-packed with the minced liver infiltrated into the spaces of the remnant liver tissue even after 60 days, by exhibiting a similar configuration of the liver cell cord with the division, proliferation and fusing. The intercellular space of the sinusoid resemblance was also admitted between each hepatic stem-like cell. Stained also positive with PAS staining, it was suggested that these transformed hepatic stem-like cells have the metabolic ability of glucose.
The study showing that hepatic stem-like cells were differentiated into the resemblance of the true liver tissue, indicated the prospect of the creation for the internal auxiliary liver.

Research Products

• [Journal Article] Reconfirmation of the anatomy of the left triangular ligament and the appendix fibrosa hepatis in human livers, and its implication in abdominal surgery (2014)
  • Author(s): Kimitaka Kogure, Itaru Kojima, Hiroyuki Kuwano, Toshiyuki Matsuzaki, Hiroshi Yorifuji, Kuniaki Takata, Masatoshi Makuuchi
  • Journal Title: Journal of Hepato-Biliary Pancreatic Science Volume: 21 Issue: 12 Pages: 856-863
  • DOI: 10.1002/jhbp.144
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 有茎分節腸管内肝組織片充填式補助肝臓の開発―第１報 (2013)
  • Author(s): 小暮公孝、石崎政利、根本雅明、桑野博行、小島 至、磯村寛樹、星野洪郎、幕内雅敏
  • Journal Title: THE KITAKANTO MEDICAL JOURNAL(北関東医学) Volume: 63(2) Pages: 133-140
  • NAID: 130003368238
  • Peer Reviewed
• [Journal Article] 有茎分節腸管内肝組織片充填式補助肝臓の開発(第２報)―大網共充填の効果― (2013)
  • Author(s): 小暮公孝、石崎政利、根本雅明、桑野博行、小島 至、磯村寛樹、星野洪郎、幕内雅敏
  • Journal Title: THE KITAKANTO MEDICAL JOURNAL(北関東医学) Volume: 63(3) Pages: 223-232
  • NAID: 130003368247
  • Peer Reviewed
• [Presentation] 尾状葉肝静脈系と尾状葉notchの関係について (2015)
  • Author(s): 小暮公孝
  • Organizer: 第１９回臨床解剖研究会
  • Place of Presentation: 東京、東京大学山上会館
  • Year and Date: 2015-11-14
  • Invited
• [Presentation] 肝区域の再考察 (2015)
  • Author(s): 小暮公孝
  • Organizer: 第３回肝臓外科解剖検討会
  • Place of Presentation: 東京、東京女子医科大学病院臨床講堂２
  • Year and Date: 2015-08-22
  • Invited
• [Presentation] 左三角間膜(left triangular ligament: LTL)と肝繊維付属（appendix fibrosa hepatis: AFH）の解剖学的関係の再確認―腹部外科における要点― (2015)
  • Author(s): 小暮公孝、小島 至、石崎政利、根本雅明、桑野博之、松崎利行、依藤 宏、高田邦昭、磯村寛樹、幕内雅敏
  • Organizer: 第115回日本外科学会定期学術集会
  • Place of Presentation: 名古屋市（名古屋国際会議場）
  • Year and Date: 2015-04-16
• [Presentation] 有茎小腸グラフト内充填肝組織片の生着延長法の工夫 (2014)
  • Author(s): 小暮公孝、小島 至、石崎政利、根本雅明、久保憲生、鈴木秀樹、桑野博行、磯村寛樹、星野洪郎、幕内雅敏
  • Organizer: 第114回日本外科学会総会
  • Place of Presentation: 京都府京都市国立京都国際会館
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 門脈血流を考慮した肝組織片充填有茎小腸グラフトの開発 (2013)
  • Author(s): 小暮公孝、小島 至、久保憲生、新木健一郎、持田 泰、鈴木秀樹、桑野博行、磯村寛樹、星野洪郎、幕内雅敏
  • Organizer: 第113回日本外科学会総会
  • Place of Presentation: 福岡市 福岡国際会議場