| Project/Area Number |
25670595
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Cardiovascular surgery
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
HOSOYAMA Tohru 山口大学, 医学(系)研究科(研究院), 助教 (20638803)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
LI Tao-Sheng 長崎大学, 原爆後障害医療研究所, 教授 (50379997)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | ヒト多能性幹細胞 / 心筋前駆細胞 / 骨格筋前駆細胞 / ヒトiPS細胞 |
|---|
| Outline of Final Research Achievements |
Specific Aim of this study was to clarify whether human induced pluripotent stem cell (hiPSCs)-derived skeletal muscle progenitor cells (SMPCs) can become novel cell supplier for cardiac progenitor cells (CPC). In this study, we particularly focused on the microRNA-associated regulation and the multipotency of SMPCs. As a result, we demonstrated that hiPSCs-derived SMPCs, termed EZ spheres, potentiate multi-differentiation capacities and transplanted EZ spheres can give rise to cardiomyocytes in mouse infarcted heart. While, an inhibition of miR-1 family did not affect the multipotency of EZ spheres. Taken together, this study clearly demonstrated that hiPSCs-derived EZ spheres is valuable cell source as a bipotent progenitor cells to differentiate into both skeletal and heart muscles, and it is expected that microRNA-mediated regulation in the multipotency of EZ spheres in future studies.
|
