Mechanism of transcriptional regulation from arachnoid cells to endothelial cells

• Project/Area Number: 25670619
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neurosurgery
• Research Institution: The University of Tokyo
• Principal Investigator: WADA Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033)
• Project Period (FY): 2013-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 脳血管障害 / トランスクリプトーム / エピゲノム / くも膜

Research Abstract

It is not fully elucidated the role of arachnoid cells in the course of subarachnoid hemorrhage. It was found that arachnoid cells could contribute to the recovery from cerebrovascular diseases. To elucidate the transcriptional regulation mechanism of arachnoid cells, we performed comprehensive transcriptional analysis followed by epigenetic analysis. We found some of histone modifications were dynamically changed, suggesting the importance of chromatin dynamics of arachnoid cells. Based on the outcome of this project, further study is now ongoing to confirm and validate the significance of these findings.

Research Products

• [Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells. (2014)
  • Author(s): Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
  • Journal Title: PLoS One. Volume: 9 Issue: 5 Pages: e96005-e96005
  • DOI: 10.1371/journal.pone.0096005
  • Peer Reviewed / Open Access
• [Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements (2014)
  • Author(s): Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y
  • Journal Title: Genome Biol. Volume: 15(4)[Epub ahead of print]
  • Peer Reviewed
• [Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements. (2014)
  • Author(s): Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
  • Journal Title: Genome Biology Volume: 15 Issue: 4
  • DOI: 10.1186/gb-2014-15-4-r63
  • Peer Reviewed / Open Access
• [Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells. (2014)
  • Author(s): Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi K, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Sugiyama A, Nozaki N, Aburatani H, Nangaku M, Ruan X, Tanabe H, Ruan T, Ihara S, Endo A, Kodama T, *Wada Y （他12名）
  • Journal Title: PLoS One Volume: 印刷中
  • NAID: 120006667792
  • Peer Reviewed
• [Journal Article] Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli (2014)
  • Author(s): Imamura K, Imamachi N, Akizuki G, Kumakura M, Kawaguchi A, Nagata K, Kato A, Kawaguchi Y, Sato H, Yoneda M, Kai C, Yada T, Suzuki Y, Yamada T, Ozawa T, Kaneki K, Inoue T, Kobayashi M, Kodama T, Wada Y, Sekimizu K and Akimitsu N.
  • Journal Title: Molecular cell Volume: 53 Issue: 3 Pages: 393-406
  • DOI: 10.1016/j.molcel.2014.01.009
  • Peer Reviewed / Open Access
• [Presentation] 内皮細胞において観察される転写の波が示唆する転写複合体によるクロマチン構造変化 (2013)
  • Author(s): 和田洋一郎
  • Organizer: 日本遺伝学会
  • Place of Presentation: 慶応大学日吉キャンパス(神奈川)
  • Year and Date: 2013-03-27