MS imaging Analysis to identify protein expressions regulating malignant transformation in glioma

• Project/Area Number: 25670623
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery
• Research Institution: Kyoto University
• Principal Investigator: SUSUMU Miyamoto 京都大学, 医学(系)研究科(研究院), 教授 (70239440)
• Co-Investigator(Kenkyū-buntansha): ARAKAWA Yoshiki 京都大学, 大学院医学研究科, 助教 (20378649)
• Co-Investigator(Renkei-kenkyūsha): FUMIHIKO Matsuda 京都大学, 大学院医学研究科, 教授 (50212220)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 神経膠腫 / 悪性転化 / MSイメージング / 質量分析 / グリオーマ / 質量分析解析 / 質量顕微鏡 / mass spectrometry / エピジェネティック

Outline of Final Research Achievements

The aim of the research project is to screen protein expressions associated with malignant transformation in glioma, to identify the driver proteins, and to reveal the novel mechanism. We evaluated the pretreatment conditions for MS imaging and visualized the protein expressions of clinical samples. To identify the proteins detected by MS imaging, the pretreatment conditions for LC/MS/MS analysis was examined. As preliminary results, we identified some proteins which has been known to be involved in gliomagenesis.

Research Products

• [Journal Article] Prognostic prediction of glioblastoma by quantitative assessment of the methylation status of the entire MGMT promoter region (2014)
  • Author(s): 2.Manabu Kanemoto, Mitsuaki Shirahata, Akiyo Nakauma, Katsumi Nakanishi, Kazuya Taniguchi, Yoji Kukita, Yoshiki Arakawa, Susumu Miyamoto、 Kikuya Kato
  • Journal Title: BMC Cancer Volume: 14 Issue: 1 Pages: 641-651
  • DOI: 10.1186/1471-2407-14-641
  • Peer Reviewed / Open Access
• [Journal Article] Retrospective Analysis of Bevacizumab in Combination With Ifosfamide, Carboplatin, and Etoposide in Patients With Second Recurrence of Glioblastoma (2013)
  • Author(s): oshiki Arakawa, Takashi Mizowaki, Daiki Murata, Koichi Fujimoto, Takayuki Kikuchi, Takeharu Kunieda, Jun C. Takahashi, Yasushi Takagi, Susumu Miyamoto.
  • Journal Title: Neurologia medico-chirurgica Volume: 53 Issue: 11 Pages: 779-785
  • DOI: 10.2176/nmc.oa2013-0211
  • NAID: 10031196789
  • ISSN: 0470-8105, 1349-8029
  • Peer Reviewed
• [Presentation] 質量顕微鏡を用いたグリオーマのプロテオーム解析 (2014)
  • Author(s): 藤本浩一、荒川芳輝、矢島由佳、鶴山竜昭、福井伸行、村田大樹、國枝武治、高木康志、松田文彦、宮本 享
  • Organizer: 第15回日本分子脳神経外科学会
  • Place of Presentation: 大手門パルズ 山形市
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] 再々発膠芽腫に対するbevacizumab 併用ICE 療法の治療経験と多施設共同試験の提案 (2013)
  • Author(s): 荒川芳輝、青木友和、上羽哲也、北条雅人、岩崎孝一、丹治正大、國枝武治、高橋淳Ｂ、髙橋淳Ｃ、高木康志、宮本享
  • Organizer: 第31回日本脳腫瘍学会学術集会
  • Place of Presentation: 宮崎