| Project/Area Number |
25670659
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIDA Kentaro 北里大学, 医学部, 助教 (50547578)
MATSUSHITA Osamu 岡山大学, 医歯(薬)学総合研究科, 教授 (00209537)
MABUCHI Yo 東京医科歯科大学, その他の研究科, 助教 (50424172)
INOUE Gen 北里大学, 医学部, 准教授 (80594209)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 人工骨膜 / コラーゲン結合型成長因子 / 間葉系幹細胞 / 細胞積層技術 / 人工骨膜組織 / 機能性人工骨膜 |
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| Outline of Final Research Achievements |
We attempted to develop a functional artificial periosteum using mesenchymal stem cells (MSCs) and a fusion protein (collagen binding-bFGF, CB-bFGF) consisting of bFGF and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase for bone repair. Artificial periosteum had higher expression of trophic factots than monolayer cultured MSCs. Moreover, the combination of the collage CB-bFGF with artificial periosteum induced bone formation compared to artificial periosteum alone in rat models. Taken together, these properties suggest that the CB-bFGF/artificial periosteum composite is a promising material for bone repair in the clinical setting.
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