Development of novel fracture healing methods using artificical perioteum and collagen-binding growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670659
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Kitasato University
• Principal Investigator: TAKASO Masashi 北里大学, 医学部, 教授 (90439117)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Kentaro 北里大学, 医学部, 助教 (50547578) ; MATSUSHITA Osamu 岡山大学, 医歯(薬)学総合研究科, 教授 (00209537) ; MABUCHI Yo 東京医科歯科大学, その他の研究科, 助教 (50424172) ; INOUE Gen 北里大学, 医学部, 准教授 (80594209)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 人工骨膜 / コラーゲン結合型成長因子 / 間葉系幹細胞

Outline of Final Research Achievements

We attempted to develop a functional artificial periosteum using mesenchymal stem cells (MSCs) and a fusion protein (collagen binding-bFGF, CB-bFGF) consisting of bFGF and the collagen-binding domain (CBD) of Clostridium histolyticum collagenase for bone repair. Artificial periosteum had higher expression of trophic factots than monolayer cultured MSCs. Moreover, the combination of the collage CB-bFGF with artificial periosteum induced bone formation compared to artificial periosteum alone in rat models. Taken together, these properties suggest that the CB-bFGF/artificial periosteum composite is a promising material for bone repair in the clinical setting.

Free Research Field

運動器再生医療