The application to image diagnosis of prostate cancer by metabolomic analysis using high resolution imaging mass spectorometry

• Project/Area Number: 25670681
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: TOMOMI Kamba 京都大学, 医学(系)研究科(研究院), 准教授 (20402836)
• Co-Investigator(Kenkyū-buntansha): YAMASAKI Toshinari 京都大学, 医学研究科, 助教 (00607749) ; TERADA Naoki 京都大学, 医学研究科, 助教 (60636637)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 前立腺癌 / バイオマーカー / メタボロミクス / 質量顕微鏡 / リン脂質 / メタボローム

Outline of Final Research Achievements

Prostate specific antigen (PSA) is widely accepted as a serum biomarker for the diagnosis of PCa. However, due to its low specificity and lack of relevance with aggressiveness, novel biomarkers to complement PSA are needed. Our aim was to identify new biomarker for prostate cancer by metabolomic analysis using high resolution imaging mass spectrometry. And we aimed at application to diagnostic imaging of new biomarker candidate.
We identified that the expression of three phosphatidylinositoles (PIs) was significantly higher in cancer tissue than in benign epithelium. These differences in PI expression profiles may serve as a novel diagnostic tool for prostate cancer. However, it was difficult to recognize the difference of these PIs' expression between cancer and benign epithelium by using Magnetic Resonance Spectrometry（MRS). We are plannning to investigate these PIs function.

Research Products

• [Journal Article] The expression profile of phosphatidylinositol in high spatial resolution imaging mass spectrometry as a potential biomarker for prostate cancer (2014)
  • Author(s): Goto T, Terada N, Inoue T, Nakayama K, Okada Y, Yoshikawa T, Miyazaki Y, Uegaki M, Sumiyoshi S, Kobayashi T, Kamba T, Yoshimura K, Ogawa O
  • Journal Title: PLoS One Volume: 9(2) Issue: 2 Pages: e90242-e90242
  • DOI: 10.1371/journal.pone.0090242
  • Peer Reviewed
• [Presentation] 高解像度質量顕微鏡を用いたin situ脂質発現プロファイル解析による前立腺癌バイオマーカー探索 (2014)
  • Author(s): 後藤 崇之、寺田 直樹、井上 貴博、宮﨑 有、植垣 正幸、吉川 武志、岡田 能幸、小林 恭、山崎 俊成、神波 大己、小川 修
  • Organizer: 第73回 日本癌学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] The expression profile of phosphatidylinositol in high resolution imaging mass spectrometry is a potential biomarker for prostate cancer. (2014)
  • Author(s): Takayuki Goto, Naoki Terada, Takahiro Inoue, Kenji Nakayama, Takeshi Yoshikawa, Yu Miyazaki, Masayuki Uegaki, Takashi Kobayashi, Tomomi Kamba, Koji Yoshimura, Osamu Ogawa
  • Organizer: 29th Annual EAU (European Association of Urology) Congress
  • Place of Presentation: Stockholm
  • Year and Date: 2014-04-11 – 2014-04-15
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2014)
  • Author(s): Takayuki Goto, Naoki Terada, Takahiro Inoue, Kenji Nakayama, Yu Miyazaki, Masayuki Uegaki, Takeshi Yoshikawa, Takashi Kobayashi, Tomomi Kamba, Koji Yoshimura, Osamu Ogawa
  • Organizer: The 4th Congress of Asian Pacific Prostate Society
  • Place of Presentation: Okinawa, JAPAN
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織のin situ 脂質プロファイリング (2014)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎有、植垣正幸、吉川武志、小林恭、神波大己、吉村耕治、小川修
  • Organizer: 第23回泌尿器科分子細胞研究会
  • Place of Presentation: 山形
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2013)
  • Author(s): 後藤崇之、寺田直樹、井上貴博、中山憲司、宮崎 有、植垣正幸、吉川武志、小林恭、清水洋祐、神波大己、吉村耕治、小川修
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜