Analysis of the functions of a molecular chaperon HSP70 during pregnancy, and attempt to find new treatments of premature labor.

• Project/Area Number: 25670696
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: YAMASHITA TAKAHIRO 東京大学, 医学部附属病院, 登録研究員 (90313147)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 分子シャペロン / HSP / 早産

Outline of Final Research Achievements

Teprenone has been reported to have an effect to induce HSP70. We tried to analyze whether Teprenone is effective or not to prevent preterm labor. At first, we used choriocarcinoma cell line, BeWo. We tried to measure HSP70 concentration in the culture supernate of BeWo. However, we could not detect it in analyzable concentration. Other cell lines, JAR, JEG-3, showed similar results. Next, we tried to analyze the effect of Teprenone on the invasive and proliferative capacity of BeWo, which also was not significant. We tried to detect the expression of HSP70 on placenta of mouse with immunohistochemical staining. However, only non-specific pattern was observed. We could not proceed to the analysis on human materials.