| Project/Area Number |
25670735
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 眼細胞生物学 / 二光子顕微鏡 / 結膜損傷 / 好中球 |
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| Outline of Final Research Achievements |
We visualized intravital inflammatory cell dynamics in mice conjunctiva in vivo by using two-photon microscopy, and evaluated immuno-reaction after conjunctival invasive interventions. Using LysM-eGFP transgenic mouse, eGFP-labeled inflammatory cells (mainly neutrophil) were tracked and imaged. In conjunctival injury model by 10-0 nylon suturing, eGFP-positive cells were significantly and time-dependently increased compared to non-injured control. Also, the migration velocities of inflammatory cells were rapidly increased by 3 hours after invasion, and sustained until 72 hours. This novel in-vivo live imaging technique may be a useful tool to elucidate the inflammatory/wound healing mechanism in conjunctiva after glaucoma surgery..
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