4-dimention live imaging of inflamatory cells in conjunctiva after glaucoma surgery

• Project/Area Number: 25670735
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kumamoto University
• Principal Investigator: TANIHARA Hidenobu 熊本大学, 生命科学研究部, 教授 (60217148)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 眼細胞生物学 / 二光子顕微鏡 / 結膜損傷 / 好中球

Outline of Final Research Achievements

We visualized intravital inflammatory cell dynamics in mice conjunctiva in vivo by using two-photon microscopy, and evaluated immuno-reaction after conjunctival invasive interventions. Using LysM-eGFP transgenic mouse, eGFP-labeled inflammatory cells (mainly neutrophil) were tracked and imaged. In conjunctival injury model by 10-0 nylon suturing, eGFP-positive cells were significantly and time-dependently increased compared to non-injured control. Also, the migration velocities of inflammatory cells were rapidly increased by 3 hours after invasion, and sustained until 72 hours. This novel in-vivo live imaging technique may be a useful tool to elucidate the inflammatory/wound healing mechanism in conjunctiva after glaucoma surgery..

Research Products

• [Journal Article] Evaluation of filtering blebs exhibiting transconjunctival oozing using anterior segment optical coherence tomography. (2015)
  • Author(s): Nakashima K, Inoue T, Fukushima A, Hirakawa S, Kojima S, Tanihara H.
  • Journal Title: Graefe's Archives of Clinical and Experimental Ophthalmology Volume: 253 Issue: 3 Pages: 439-445
  • DOI: 10.1007/s00417-014-2872-3
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efficacy and safety of SNJ-1656 in primary open-angle glaucoma or ocular hypertension. (2015)
  • Author(s): Inoue T, Tanihara H, Tokushige H, Araie M.
  • Journal Title: Acta Ophthalmologica Volume: 印刷中 Issue: 5
  • DOI: 10.1111/aos.12641
  • Peer Reviewed
• [Journal Article] Additive Intraocular Pressure-Lowering Effects of the Rho Kinase Inhibitor Ripasudil (K-115) Combined With Timolol or Latanoprost: A Report of 2 Randomized Clinical Trials. (2015)
  • Author(s): Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Suganami H, Araie M; K-115 Clinical Study Group.
  • Journal Title: JAMA Ophthalmology Volume: 印刷中 Issue: 7 Pages: 755-761
  • DOI: 10.1001/jamaophthalmol.2015.0525
  • Peer Reviewed / Open Access
• [Journal Article] Trabeculectomy for open-angle glaucoma in phakic eyes vs in pseudophakic eyes after phacoemulsification : a prospective clinical cohort study (2014)
  • Author(s): Takihara Y, Inatani M, Ogata-Iwao M, Kawai M, Inoue T, Iwao K, Tanihara H
  • Journal Title: JAMA Ophthalmology Volume: 132 Issue: 1 Pages: 69-76
  • DOI: 10.1001/jamaophthalmol.2013.5605
  • Peer Reviewed
• [Journal Article] Epithelial mesenchymal transition-like phenomenon in trabecular meshwork cells (2014)
  • Author(s): Takahashi E, Inoue T, Fujimoto T, Kojima S, Tanihara H
  • Journal Title: Experimental Eye Research Volume: 118 Pages: 72-79
  • DOI: 10.1016/j.exer.2013.11.014
  • Peer Reviewed
• [Journal Article] The 5-year incidence of bleb-related infection and its risk factors following filtering surgeries with adjunctive mitomycin C (2014)
  • Author(s): Yamamoto T,et al
  • Journal Title: Collaborative Bleb-related Infection Incidence and Treatment Study 2 Ophthalmology Volume: 121(5) Issue: 5 Pages: 1001-1006
  • DOI: 10.1016/j.ophtha.2013.11.025
  • Peer Reviewed
• [Journal Article] Tear fluid signs associated with filtration blebs, as demonstrated by three-dimensional anterior segment optical coherence tomography (2014)
  • Author(s): Kojima S, Inoue T, Kawaji T, Tanihara H
  • Journal Title: Clinical Ophthalmology Volume: 8 Pages: 767-772
  • DOI: 10.2147/opth.s59778
  • Peer Reviewed / Open Access
• [Journal Article] Long-term outcomes and complications of trabeculectomy for secondary glaucoma in patients with familial amyloidotic polyneuropathy (2014)
  • Author(s): Kawaji T, Inoue T, Hara R, Eiki D, Ando Y, Tanihara H
  • Journal Title: PLoS One Volume: 9 Issue: 5 Pages: e96324-e96324
  • DOI: 10.1371/journal.pone.0096324
  • Peer Reviewed / Open Access
• [Journal Article] Fornix-based versus limbal-based conjunctival flaps in trabeculectomy with mitomycin C in high-risk patients. (2014)
  • Author(s): Kuroda U, Inoue T, Awai-Kasaoka N, Shobayashi K, Kojima S, Tanihara H.
  • Journal Title: Clinical Ophthalmology Volume: 8 Pages: 949-954
  • DOI: 10.2147/opth.s61342
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Filtering blebs using 3-dimensional anterior-segment optical coherence tomography: a prospective investigation. (2014)
  • Author(s): Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H.
  • Journal Title: JAMA Ophthalmology Volume: 133 Issue: 2 Pages: 148-156
  • DOI: 10.1001/jamaophthalmol.2014.4489
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intra-ocular pressure-lowering effects of a Rho kinase inhibitor, ripasudil (K-115), over 24 hours in primary open-angle glaucoma and ocular hypertension: a randomized, open-label, crossover study. (2014)
  • Author(s): Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Suganami H, Araie M; the K-115 Clinical Study Group.
  • Journal Title: Acta Ophthalmologica Volume: 93 Issue: 4 Pages: 254-260
  • DOI: 10.1111/aos.12599
  • Peer Reviewed
• [Journal Article] p38 MAP Kinase Inhibitor Suppresses Transforming Growth Factor-β2-Induced Type 1 Collagen Production in Trabecular Meshwork Cells. (2014)
  • Author(s): Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: 1-13
  • DOI: 10.1371/journal.pone.0120774
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Long-term outcomes and prognostic factors for trabeculectomy with mitomycin C in eyes with uveitic glaucoma : a retrospective cohort study (2014)
  • Author(s): Iwao K, Inatani M, Seto T, Takihara Y, Ogata-Iwao M, Okinami S, Tanihara H
  • Journal Title: Journal of Glaucoma Volume: 23 Issue: 2 Pages: 88-94
  • DOI: 10.1097/ijg.0b013e3182685167
  • Peer Reviewed
• [Journal Article] Phase 1 clinical trials of a selective Rho kinase inhibitor, K-115. (2013)
  • Author(s): Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Araie M; K-115 Clinical Study Group.
  • Journal Title: JAMA Ophthalmol Volume: 131(10) Issue: 10 Pages: 1288-1295
  • DOI: 10.1001/jamaophthalmol.2013.323
  • Peer Reviewed
• [Journal Article] Monocyte chemotactic protein-1 level in the aqueous humor as a prognostic factor for the outcome of trabeculectomy (2013)
  • Author(s): Inoue T, Kawaji T, Tanihara H
  • Journal Title: Clinical and Experimental Ophthalmology Volume: (in press) Issue: 4 Pages: 334-341
  • DOI: 10.1111/ceo.12204
  • Peer Reviewed
• [Journal Article] Changes in visual acuity and intra-ocular pressure following bleb-related infection: the Japan Glaucoma Society Survey of Bleb-related Infection Report 2 (2013)
  • Author(s): Yamamoto T, Kuwayama Y, Nomura E, Tanihara H, Mori K; Japan Glaucoma Society Survey of Bleb-related Infection
  • Journal Title: Acta Ophthalmologica Volume: 91 Issue: 6 Pages: 420-426
  • DOI: 10.1111/aos.12079
  • Peer Reviewed
• [Journal Article] A prospective comparison between trabeculectomy with mitomycin C and phacotrabeculectomy with mitomycin C (2013)
  • Author(s): Ogata-Iwao M, Inatani M, Takihara Y, Inoue T, Iwao K, Tanihara H
  • Journal Title: Acta Ophthalmologica Volume: 91 Issue: 6 Pages: 500-501
  • DOI: 10.1111/aos.12133
  • Peer Reviewed
• [Journal Article] Rho-associated kinase inhibitors: a novel glaucoma therapy (2013)
  • Author(s): Inoue T, Tanihara H
  • Journal Title: Progress in Retinal and Eye Research Volume: 37 Pages: 1-12
  • DOI: 10.1016/j.preteyeres.2013.05.002
  • Peer Reviewed
• [Journal Article] Oxidative stress response signaling pathways in trabecular meshwork cells and their effects on cell viability (2013)
  • Author(s): Awai-Kasaoka N, Inoue T, Kameda T, Fujimoto T, Inoue-Mochita M, Tanihara H
  • Journal Title: Molecular Vision Volume: 19 Pages: 1332-1340
  • Peer Reviewed
• [Journal Article] Phase 2 randomized clinical study of a Rho kinase inhibitor, K-115, in primary open-angle glaucoma and ocular hypertension (2013)
  • Author(s): Tanihara H, Inoue T, Yamamoto T, Kuwayama Y, Abe H, Araie M; K-115 Clinical Study Group
  • Journal Title: American Journal of Ophthalmology Volume: 156 Issue: 4 Pages: 731-736
  • DOI: 10.1016/j.ajo.2013.05.016
  • Peer Reviewed
• [Journal Article] Elevated levels of multiple biomarkers of Alzheimer's disease in the aqueous humor of eyes with open-angle glaucoma (2013)
  • Author(s): Inoue T, Kawaji T, Tanihara H
  • Journal Title: Investigative Ophthalmology & Visual Science Volume: 54 Issue: 8 Pages: 5353-5358
  • DOI: 10.1167/iovs.13-12245
  • Peer Reviewed
• [Journal Article] Risk factors for choroidal detachment after trabeculectomy with mitomycin C (2013)
  • Author(s): Haga A, Inatani M, Shobayashi K, Kojima S, Inoue T, Tanihara H
  • Journal Title: Clinical Ophthalmology Volume: 7 Pages: 1417-1421
  • DOI: 10.2147/opth.s46375
  • Peer Reviewed
• [Journal Article] Study on prognostic factor in trabeculectomy with mitomycin C having history of previous glaucoma surgery (2013)
  • Author(s): Awai-Kasaoka N, Inoue T, Inatani M, Takihara Y, Ogata-Iwao M, Tanihara H
  • Journal Title: Japanese Journal of Ophthalmology Volume: 57 Issue: 6 Pages: 514-519
  • DOI: 10.1007/s10384-013-0257-5
  • Peer Reviewed
• [Presentation] Prospective investigation of filtering bleb by three-dimensional anterior-segment optical coherence tomography. (2014)
  • Author(s): Inoue T, Kojima S, Fukushima A, Nakashima K, Tanihara H.
  • Organizer: European Glaucoma Society 2014
  • Place of Presentation: Nice, France
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] Postoperative changes in the aqueous monocyte chemotactic protein-1 (MCP-1) level after trabeculectomy vs. Ex-PRESS shunt. (2014)
  • Author(s): Shobayashi K, Inoue T, Kawai M, Tanihara H.
  • Organizer: European Glaucoma Society 2014
  • Place of Presentation: Nice, France
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] Immune cell dynamics around the subconjunctival tissue after injury by four dimensional in vivo imaging. (2014)
  • Author(s): Kojima S, Inoue T, Tanihara H.
  • Organizer: European Glaucoma Society 2014
  • Place of Presentation: Nice, France
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] Live cell imaging of F-actin in dexamethasone treated trabecular meshwork cells. (2014)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Tanihara H.
  • Organizer: European Glaucoma Society 2014
  • Place of Presentation: Nice, France
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] Prospective investigation of filtering bleb by three-dimensional anterior-segment optical coherence tomography. (2014)
  • Author(s): Inoue T, Kojima S, Fukushima A, Nakashima K, Tanihara H.
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Effects of p38 inhibitor on TGF-β2 signal in trabecular meshwork cells. (2014)
  • Author(s): Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H.
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Live cell imaging of F-actin in dexamethasone treated trabecular meshwork cells. (2014)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Tanihara H.
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Neovascular Glaucoma. (2014)
  • Author(s): Ohira S, Inoue T, Tanihara H.
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO)
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2014-05-04 – 2014-05-08
• [Presentation] K-115 Clinical Study Group. Phase 3 randomized clinical study of a Rho kinase inhibitor, K-115, in combination with timolol or latanoprost in primary open-angle glaucoma and ocular hypertension. (2014)
  • Author(s): Tanihara H, Yamamoto T, Kuwayama Y, Abe H, Inoue T, Araie M
  • Organizer: World Ophthalmology Congress 2014
  • Place of Presentation: Tokyo international forum, Tokyo, Japan
  • Year and Date: 2014-04-02 – 2014-04-06
• [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma (2013)
  • Author(s): Inoue T, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography (2013)
  • Author(s): Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Identification of TGF-β2-induced proinflammatory cytokines secreted from cultured trabecular meshwork cells (2013)
  • Author(s): Inoue-Mochita M, Inoue T, Fujimoto T, Awai-Kasaoka N, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Evaluation of filtering blebs with transconjunctival oozing by anterior segment optical coherence tomography (2013)
  • Author(s): Nakashima K, Inoue T, Fukushima A, Hirakawa S, Kawaji T, Tanihara H
  • Organizer: The Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seattle, Washington, USA
• [Presentation] Investigation of Multiple Proinflammatory Cytokines in the Aqueous Humor in Eyes with Secondary Glaucoma (2013)
  • Author(s): Inoue T, Tanihara H
  • Organizer: World Glaucoma Congress 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] Short-term prospective investigation of filtering bleb by three dimensional anterior-segment optical coherence tomography (2013)
  • Author(s): Kojima S, Inoue T, Nakashima K, Fukushima A, Tanihara H
  • Organizer: World Glaucoma Congress 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] Visualization of actin filament using time-lapse fluorescent microscopy in trabecular meshwork cells (2013)
  • Author(s): Fujimoto T, Inoue T, Inoue-Mochita M, Kasaoka N, Shobayashi K, Tanihara H
  • Organizer: World Glaucoma Congress 2013
  • Place of Presentation: Vancouver, Canada
• [Presentation] A short-term efficacy and safety of brimonidine 0.1% as fourth-line therapy in open-angle glaucoma subjects (2013)
  • Author(s): Hirakawa S, Kojima S, Inoue T, Tanihara H
  • Organizer: World Glaucoma Congress 2013
  • Place of Presentation: Vancouver, Canada
• [Remarks] 熊本大学大学院生命科学研究部眼科学分野 研究内容
  • URL: http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/naiyou.html
• [Remarks] 熊本大学大学院生命科学研究部眼科学分野 研究業績
  • URL: http://www2.kuh.kumamoto-u.ac.jp/ganka/kyousitu/gyouseki.html