Micro-anatomy atlas using quantitative protein expression analysis
Project/Area Number |
25670750
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Plastic surgery
|
Research Institution | Kobe University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
NOMURA TADASHI 神戸大学, 医学部附属病院, 特命講師 (30529566)
SAKAKIBARA SHUNSUKE 神戸大学, 医学研究科, 特定助教 (50444592)
TAKASU HIROYUKI 神戸大学, 医学研究科, 特定助教 (40566022)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | 顔面神経損傷 / Hn-1 / 解剖学 / 3次元構築 / 顔面神経 / HN-1 / 神経再生 / 神経損傷 / HN1 |
Outline of Final Research Achievements |
Whole rat brain mRNA was extracted and amplified a part of the rat (Wistar rat) Hn-1 gene by the polymerase chain reaction. After performing the sequence analysis, we confirmed that this was known Hn-1 gene with homology. cRNA probe was constructed and in situ hybridization was performed for a rat brain slice after the unilateral facial nerve damage using this cRNA probe. Expression of Hn-1 mRNA was observed at facial nerve nucleus and trigeminal nerve nucleous on the damaged side.
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Report
(4 results)
Research Products
(11 results)