Micro-anatomy atlas using quantitative protein expression analysis

• Project/Area Number: 25670750
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kobe University
• Principal Investigator: Hashikawa Kazunobu 神戸大学, 医学部附属病院, 准教授 (90403237)
• Co-Investigator(Kenkyū-buntansha): NOMURA TADASHI 神戸大学, 医学部附属病院, 特命講師 (30529566) ; SAKAKIBARA SHUNSUKE 神戸大学, 医学研究科, 特定助教 (50444592) ; TAKASU HIROYUKI 神戸大学, 医学研究科, 特定助教 (40566022)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 顔面神経損傷 / Hn-1 / 解剖学 / 3次元構築 / 顔面神経 / HN-1 / 神経再生 / 神経損傷 / HN1

Outline of Final Research Achievements

Whole rat brain mRNA was extracted and amplified a part of the rat (Wistar rat) Hn-1 gene by the polymerase chain reaction. After performing the sequence analysis, we confirmed that this was known Hn-1 gene with homology. cRNA probe was constructed and in situ hybridization was performed for a rat brain slice after the unilateral facial nerve damage using this cRNA probe. Expression of Hn-1 mRNA was observed at facial nerve nucleus and trigeminal nerve nucleous on the damaged side.

Research Products

• [Journal Article] Four-dimensional computed tomography evaluation of jaw movement following mandibular reconstruction: A pilot study (2016)
  • Author(s): Akashi M, Shibuya Y, Takahashi S, Hashikawa K, Hasegawa T, Kakei Y, Negi N, Sekitani T, Komori T.
  • Journal Title: J Craniomaxillofac Surg. Volume: 44(5) Issue: 5 Pages: 637-41
  • DOI: 10.1016/j.jcms.2016.01.027
  • Peer Reviewed
• [Journal Article] CT Evaluation of Morphology of Transferred Fibula for Implant Placement in Reconstructed Mandible (2015)
  • Author(s): Akashi M, Shibuya Y, Wanifuchi S, Kusumoto J, Sakakibara A, Kimoto A, Hasegawa T, Suzuki H, Hashikawa K, Komori T.
  • Journal Title: Implant Dent. Volume: 24(5) Issue: 5 Pages: 541-6
  • DOI: 10.1097/id.0000000000000290
  • Peer Reviewed
• [Journal Article] “Sensory Switching” in Elbow Reconstruction (2015)
  • Author(s): S Sakakibara, K Hashikawa, and H Terashi
  • Journal Title: Journal of Brachial Plexus and Peripheral Nerve Injury Volume: 未定 Issue: 01 Pages: e30-e33
  • DOI: 10.1055/s-0035-1549369
  • Peer Reviewed / Open Access
• [Journal Article] Development of a novel method for decellularizing a nerve graft using a hypertonic sodium chloride solution. (2014)
  • Author(s): Ishida Y, Sakakibara S, Terashi H, Hashikawa K, Yamaoka TY
  • Journal Title: Int J Artif Organs Volume: 37 Issue: 11 Pages: 169-77
  • DOI: 10.5301/ijao.5000365
  • Peer Reviewed
• [Journal Article] 発症後長期経過した顔面神経不全麻痺例に対するクロスリンク型神経移植術 (2014)
  • Author(s): 橋川和信
  • Journal Title: Facial Nerve Research Volume: 34 Pages: 32-34
  • Peer Reviewed
• [Journal Article] 陳旧性顔面神経不全麻痺に対するクロスリンク型神経移植術 (2014)
  • Author(s): 橋川和信
  • Journal Title: PEPARS Volume: 92 Pages: 87-93
• [Journal Article] 顔面神経麻痺再建法における定義ならびに呼称～Fukushima提言～ (2014)
  • Author(s): 林礼人, 山本有平, 垣淵正男, 松田健, 古川洋志, 橋川和信, 渡辺頼勝, 上田和毅
  • Journal Title: 日形会誌. Volume: 34 Pages: 783-796
  • Peer Reviewed
• [Presentation] 血管・神経・リンパ管のマイクロサージャリー―初心者からステップアップするためのポイント― (2014)
  • Author(s): 橋川和信
  • Organizer: 第6回日本創傷外科学会総会・学術集会,
  • Place of Presentation: 高松
  • Year and Date: 2014-07-24 – 2014-07-25
  • Invited
• [Presentation] 発症後長期経過した顔面神経不全麻痺例に対するクロスリンク型神経移植術 (2014)
  • Author(s): 橋川和信
  • Organizer: 第37回日本顔面神経学会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-29 – 2014-05-30
• [Presentation] 顔面神経不全麻痺に対する美容外科的手術 (2014)
  • Author(s): 橋川和信, 寺師浩人
  • Organizer: 第57回日本形成外科学会総会・学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2014-04-09 – 2014-04-11
  • Invited
• [Presentation] Bell麻痺・Hunt症候群不全麻庫例に対するクロスリンク型神経移植術 (2013)
  • Author(s): 橋川和信
  • Organizer: 第36回日本顔面神経研究会
  • Place of Presentation: 那覇