| Project/Area Number |
25670756
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
OKOCHI Hitoshi 独立行政法人国立国際医療研究センター, その他部局等, 部長 (30185235)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 毛乳頭細胞 / 再生医学 |
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| Outline of Final Research Achievements |
Human dermal papilla cells (hDPC) were reported to express Sox2, Klf4, c-Myc which are key components for generating or maintaining iPS cells. Based on the similarity between hDPC and iPS cells, I hypothesized that suppressing the expression of the Oct3/4 gene in iPS cells may facilitate the differentiation of DPC from iPS cells. After I designed three different shRNA against Oct3/4 gene and applied them to the hiPS cells (253G:a gift from CiRA), I confirmed the expression level of the Oct3/4 gene was less than 30% of control group. Although I detected the expression of several genes specific for ectoderm, mesoderm and endoderm, I could not detect the up-regulation of DPC specific gene expression, such as versican. On the other hand I found the synergistic effect of FGF2 and PDGFAA for the growth of DPC, because addition of FGF2 to the culture media of DPC up-regulated the expression of PDGFα receptor. These results were published in Journal Dermatological Science.
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