Cell therapy using multilineage differentiating stress enduring (MUSE) cell for acute lung injury

• Project/Area Number: 25670759
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: UCHIDA Tokujiro 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (40262183)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 急性肺傷害 / MUSE細胞 / 細胞治療 / 前駆細胞

Outline of Final Research Achievements

Multilineage differentiating stress enduring cells were isolated from a human dermal fibroblast cell line. The isolated cells were soated by SSEA3 positivity, and they maintained positivity of CD105 as a surface marker. When these cells were cultivated in Methocult H4100, they formed colony shaped cell spheres. However, we could not show the therapeutic effect of these cells in mice acute lung injury model, probably because of technical failure in the maintenance of the phenotype of MUSE cells. Further studies are needed to establish efficacy of cell therapy using MUSE cells.

Research Products

• [Journal Article] Interactions between rat alveolar epithelial cells and bone marrow-derived mesenchymal stem cells: an in vitro co-culture model. (2015)
  • Author(s): Ito H, Uchida T, Makita K.
  • Journal Title: Intensive Care Med Exp. Volume: 3 Issue: 1 Pages: 53-53
  • DOI: 10.1186/s40635-015-0053-2
  • Peer Reviewed / Open Access
• [Journal Article] Ketamine causes mitochondrial dysfunction in human induced pluripotent stem cell-derived neurons. (2015)
  • Author(s): Ito H, Uchida T, Makita K
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: e0128445-e0128445
  • DOI: 10.1371/journal.pone.0128445
  • Peer Reviewed / Open Access
• [Presentation] Perioperative Elevation in Cell Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction: A Multicenter Observational Study. (2015)
  • Author(s): Yamamoto Y, Uchida T, Ito H, Kido K, Yamamoto Y, Yamada Y, Ohno N, Asahara M, Yamaguchi O, Makita K.
  • Organizer: Anesthesiology 2015
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Ketamine Causes Mitochondrial Dysfunction in Human Induced Pluripotent Stem Cell-derived Neurons (2015)
  • Author(s): Ito H, Uchida T, Makita K
  • Organizer: Anesthesiology 2015
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] Retrospective Analysis for Intra-Operative Ventilatory Management During General Anesthesia: Is Lung Protective Ventilation Accepted as a Common Practice in the Operating Room? (2015)
  • Author(s): Itoh Y, Kasuga T, Toyama S, Uchida T, Makita K
  • Organizer: Anesthesiology 2015
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-10-24
  • Int'l Joint Research
• [Presentation] ARDSの病態と治療:最新知見 (2013)
  • Author(s): 内田 篤治郎
  • Organizer: 第35回日本呼吸療法医学会学術総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Invited
• [Book] 呼吸療法UpToDate 14. ARDSの病態と治療：最新知見 (2015)
  • Author(s): 内田篤治郎
  • Total Pages: 14
  • Publisher: 真興交易
• [Book] 呼吸療法 Up Date: ARDSの病態と治療最新知見 (2015)
  • Author(s): 内田篤治郎(槇田浩史編）
  • Total Pages: 13
  • Publisher: 真興交易
• [Book] 呼吸療法 Up Date：ARDSの病態と治療:最新知見 (2014)
  • Author(s): 内田篤治郎
  • Total Pages: 6
  • Publisher: 真興交易