Development of a new therapeutic method by genome-editing
| Project/Area Number |
25670775
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kyoto University (2014)
Tokyo Medical and Dental University (2013) |
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Principal Investigator |
NAKAGAWA Ichiro 京都大学, 医学(系)研究科(研究院), 教授 (70294113)
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| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Fumito 京都大学, 大学院医学研究科微生物感染症学分野, 准教授 (30423122)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Ａ群レンサ球菌 / CRIPSR / TALEN / ZFN / 増殖抑制 / A群レンサ球菌 / CRISPR / Cas9 / ファージ / ゲノム編集 / 感染制御 |
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| Outline of Final Research Achievements |
In this study, we tried to apply the Genome Editing method on the basis of the spacer sequence information of CRISPR. The Cas9 / CRISPR system of the group A streptococci (GAS) with various spacer sequences of the chromosomal DNA were constructed, and chromosomal fragments with homologous sequence were also introduced in another compatible plasmid. CRISPR/Cas system could clearly inhibit the growth of E. coli strain with matched pair of spacer sequence and the chromosomal fragment. However, the TALEN system and ZFN system could not inhibit the growth of E. coli completely. We also construct the CIRPSR/Cas9 expression plasmid for GAS. The growth of bacteria is completely inhibited by introducing the plasmid with spacer sequence of the homologous sequence of the its chromosome. These observations indicated that the CIRPSR/Cas9 system can be used not only for the destruction of foreign DNA but also its chromosome.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Comparative genomic characterization of a Thailand-Myanmar isolate, MS6, of Vibrio cholerae O1 El Tor, which is phylogenetically related to a "US Gulf Coast" clone.2014
Author(s)
K. Okada, M. Na Ubol, W. Natakuathung, A. Roobthaisong, F. Maruyama, I. Nakagawa, S. Chantaroj, S. Hamada
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Journal Title
PLos One
Volume: 9
Issue: 6
Pages: e98120-e98120
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Bacterial Survival and Evolutional Strategies via CRISPR/Cas System2013
Author(s)
Okura M, Lachance C, Osaki M, Sekizaki T, Maruyama F, Nozawa T, Nakagawa I, Hamada S, Rossignol C, Gottschalk M, Takamatsu D
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Journal Title
Dental Medicine Research
Volume: 33
Issue: 3
Pages: 236-241
DOI
NAID
ISSN
1882-0719, 2186-540X
Related Report
Peer Reviewed
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[Journal Article] Endogenous nitrated nucleotide is a key mediator of autophagy and innate defense against bacteria.2013
Author(s)
Ito C, Saito Y, Nozawa T, Fujii S, Sawa T, Inoue H, Matsunaga T, Khan S, Akashi S, Hashimoto R, Aikawa C, Takahashi E, Sagara H, Komatsu M, Tanaka K, Akaike T, Nakagawa I, Arimoto H.
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Journal Title
Mol. Cell
Volume: 52
Issue: 6
Pages: 794-804
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Complete genome sequence of a Propionibacterium acnes isolate from a sarcoidosis patient.2013
Author(s)
Minegishi K, Aikawa C, Furukawa A, Watanabe T, Nakano T, Ogura Y, Ohtsubo Y, Kurokawa K, Hayashi T, Maruyama F, Nakagawa I, Eishi Y
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Journal Title
Genome Announcement
Volume: 1
Issue: 1
Pages: 16-12
DOI
Related Report
Peer Reviewed
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