Drug-delivery-system through ion channels
| Project/Area Number |
25670785
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
WAKAMORI Minoru 東北大学, 歯学研究科(研究院), 教授 (50222401)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Takashi 東北大学, 歯学研究科, 助教 (30455795)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | イオンチャネル / チャネル / カプサイシン / TRPV1 |
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| Outline of Final Research Achievements |
TRPV1 channel is activated by capsaicin and is permeable to Na+, K+ and Ca2+. Concentration-response relationship for capsaicin was obtained from the HEK293 cells expressing recombinant TRPV1 channel. The threshold concentration and EC50 of capsaicin were 0.03 and 0.22 uM, respectively. FM1-43, a positively charged organic ion, was a permeant when the TRPV1 channel was activated by 1 uM capsaicin, indicating that TRPV1 channel pore size is wide enough to pass the 452 Da FM1-43. When the extracellular Ca2+ was removed, the FM1-43 could not enter the cells through the TRPV1 channel. These results suggest that the TRPV1 channel pore is dilated by capsaicin but extracellular Ca2+ regulates the dilation.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Compromised maturation of GABAergic inhibition underlies abnormal network activity in the hippocampus of epileptic Ca2+ channel mutant mice, tottering.2015
Author(s)
Nakao A, Miki T, Shimono K, Oka H, Numata T, Kiyonaka S, Matsushita K, Ogura H, Niidome T, Noebels JL, Wakamori M, Imoto K, Mori Y
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Journal Title
Pflugers Arch
Volume: 467
Issue: 4
Pages: 737-752
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Inhibitory effects of AG490 on H2O2-induced TRPM2-mediated Ca(2+) entry2014
Author(s)
Shimizu S, Yonezawa R, Hagiwara T, Yoshida T, Takahashi N, Hamano S, Negoro T, Toda T, Wakamori M, Mori Y, Ishii M
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Journal Title
Eur J Pharmacol
Volume: 742
Pages: 22-30
DOI
Related Report
Peer Reviewed
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