| Project/Area Number |
25670876
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKAHAHI Ichiro 九州大学, 歯学研究科(研究院), 教授 (70241643)
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| Co-Investigator(Kenkyū-buntansha) |
TERAO FUMIE 九州大学, 大学院歯学研究院, 助教 (10510018)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 発生・分化 / microRNA / 下顎頭軟骨 / 細胞・組織 / マイクロアレイ / 一次軟骨 / 間葉系幹細胞 / マイクロRNA |
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| Outline of Final Research Achievements |
Microarray analysis using osteo-chondroprogenitor cells, putative mesenchymal stem cells in mandibular condylar cartilage and those in the primary cartilages in shoulder joint during embryonic stages has revealed that microRNA-200a (miR-200a) is one of the candidate microRNA which regulates the differentiation of secondary cartilage. We found that Dlx5 is a candidate target gene for miR-200a by microarray analysis. Reporter assay revealed that miR-200a inhibited the promoter activities of Dlx-5. Further, miR-200a reduced the production of Dlx5 protein in ATDC5 cells. Thus, it has been suggested that miR-200a regulates the differentiation of mandibular condylar cartilage cells through inhibition of Dlx5 production.
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