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Molecular basis regulating differentiation of mesenchymal stem cells in mandibular condylar cartilage by microRNAs

Research Project

Project/Area Number 25670876
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionKyushu University

Principal Investigator

TAKAHAHI Ichiro  九州大学, 歯学研究科(研究院), 教授 (70241643)

Co-Investigator(Kenkyū-buntansha) TERAO FUMIE  九州大学, 大学院歯学研究院, 助教 (10510018)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords発生・分化 / microRNA / 下顎頭軟骨 / 細胞・組織 / マイクロアレイ / 一次軟骨 / 間葉系幹細胞 / マイクロRNA
Outline of Final Research Achievements

Microarray analysis using osteo-chondroprogenitor cells, putative mesenchymal stem cells in mandibular condylar cartilage and those in the primary cartilages in shoulder joint during embryonic stages has revealed that microRNA-200a (miR-200a) is one of the candidate microRNA which regulates the differentiation of secondary cartilage. We found that Dlx5 is a candidate target gene for miR-200a by microarray analysis. Reporter assay revealed that miR-200a inhibited the promoter activities of Dlx-5. Further, miR-200a reduced the production of Dlx5 protein in ATDC5 cells. Thus, it has been suggested that miR-200a regulates the differentiation of mandibular condylar cartilage cells through inhibition of Dlx5 production.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2016 2015 2014 2013

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016

    • Author(s)
      Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
    • Journal Title

      Plos One

      Volume: 11 Issue: 3 Pages: 0152206-0152206

    • DOI

      10.1371/journal.pone.0152206

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of acidic calcium phosphate concentration on mechanical strength of porous calcite fabricated by bridging with dicalcium phosphate dihydrate.2016

    • Author(s)
      Koga N, Ishikawa K, Tsuru K, Takahashi I.
    • Journal Title

      Ceramics International.

      Volume: 42 Issue: 7 Pages: 7912-7917

    • DOI

      10.1016/j.ceramint.2015.12.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MicroRNA-200a Regulates the Development of Mandibular Condylar Cartilage2015

    • Author(s)
      M. Umeda
    • Journal Title

      Journal of dental research

      Volume: 95 Issue: 6 Pages: 795-802

    • DOI

      10.1177/0022034515577411

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of humidity on calcite block fabrication using calcium hydroxide compact.2015

    • Author(s)
      Koga N, Tsuru K, Takahashi I, Ishikawa K.
    • Journal Title

      Ceramics International.

      Volume: 41 Issue: 8 Pages: 9482-9487

    • DOI

      10.1016/j.ceramint.2015.04.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel measurement method for the morphology of the mandibular ramus using homologous modelling.2015

    • Author(s)
      Inoue K, Nakano H, Sumida T, Yamada T, Otawa N, Fukuda N, Nakajima Y, Kumamaru W, Mishima K, Kouchi M, Takahashi I, Mori Y.
    • Journal Title

      Dentomaxillofac Radiol.

      Volume: 44 Issue: 8 Pages: 20150062-20150062

    • DOI

      10.1259/dmfr.20150062

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Leptin Suppresses Mouse Taste Cell Responses to Sweet Compounds.2015

    • Author(s)
      Yoshida R, Noguchi K, Shigemura N, Jyotaki M, Takahashi I, Margolskee RF, Ninomiya Y.
    • Journal Title

      Diabetes.

      Volume: 64(11) Issue: 11 Pages: 3751-62

    • DOI

      10.2337/db14-1462

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Generic finite element models of orthodontic mini-implants: Are they reliable?2015

    • Author(s)
      Albogha MH, Takahashi I.
    • Journal Title

      J Biomech.

      Volume: 48 Issue: 14 Pages: 3751-3756

    • DOI

      10.1016/j.jbiomech.2015.08.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maximum principal strain as a criterion for prediction of orthodontic mini-implants failure in subject-specific finite element models.2015

    • Author(s)
      Albogha MH, Kitahara T, Todo M, Hyakutake H, Takahashi I.
    • Journal Title

      Angle Orthod.

      Volume: 86 Issue: 1 Pages: 24-31

    • DOI

      10.2319/120514-875.1

    • NAID

      120005649079

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Compressive force-produced CCN2 induces osteocyte apoptosis through ERK1/2 pathway2014

    • Author(s)
      K Hoshi, H Kawaki, I Takahashi, N Takeshita, M Seiryu, S A Murshid, T Masuda, T Anada, R Kato, H Kitaura, O Suzuki, T Takano-Yamamoto
    • Journal Title

      J Bone Miner Res

      Volume: 29 Issue: 5 Pages: 1244-1257

    • DOI

      10.1002/jbmr.2115

    • NAID

      130004695790

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Maxillary Distraction Osteogenesis Applied to Cleft Lip and Palate Patients with Special Consideration for Speech Function.2015

    • Author(s)
      高橋一郎
    • Organizer
      The 28th Taiwan Association of Orthodontics Annual Meeting.
    • Place of Presentation
      台湾
    • Year and Date
      2015-12-06
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 歯の発生における基底膜分子Nephronectinの役割2015

    • Author(s)
      新井智映子、吉崎恵悟、宮崎佳奈子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第74回日本矯正歯科学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胚葉異型性症原因遺伝子Plakophilin 1は歯の発生においてWntシグナル調節因子として作用する2015

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第74回日本矯正歯科学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Fabrication of interconnected porous carbonate apatite using calcite granules as a precursor.2015

    • Author(s)
      N. Koga, K. Tsuru, I. Takahashi, K. Ishikawa.
    • Organizer
      The 27th annual conference of International Society of Ceramics in Medicine (Bioceramics27)
    • Place of Presentation
      インドネシア バリ
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炭酸カルシウム連通多孔体の作製とその病理組織学的検索2015

    • Author(s)
      古賀のり子、都留寛治、高橋一郎、石川邦夫
    • Organizer
      第66回日本歯科理工学会秋期学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新規Wntシグナル調節因子PKP1の歯の発生における役割2015

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 基底膜分子Nephronectinは歯原性上皮幹細胞の分化および細胞増殖に関与する2015

    • Author(s)
      新井智映子、吉崎恵悟、宮崎佳奈子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] 胎生期マウス下顎頭軟骨における microRNA‒200a の役割2013

    • Author(s)
      梅田 まりこ、寺尾 文恵、吉崎 恵悟、宮崎 佳奈子、高橋一郎
    • Organizer
      第72回日本矯正歯科学会大会
    • Place of Presentation
      長野県松本市
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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