Molecular basis regulating differentiation of mesenchymal stem cells in mandibular condylar cartilage by microRNAs
| Project/Area Number |
25670876
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
TAKAHAHI Ichiro 九州大学, 歯学研究科(研究院), 教授 (70241643)
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| Co-Investigator(Kenkyū-buntansha) |
TERAO FUMIE 九州大学, 大学院歯学研究院, 助教 (10510018)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 発生・分化 / microRNA / 下顎頭軟骨 / 細胞・組織 / マイクロアレイ / 一次軟骨 / 間葉系幹細胞 / マイクロRNA |
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| Outline of Final Research Achievements |
Microarray analysis using osteo-chondroprogenitor cells, putative mesenchymal stem cells in mandibular condylar cartilage and those in the primary cartilages in shoulder joint during embryonic stages has revealed that microRNA-200a (miR-200a) is one of the candidate microRNA which regulates the differentiation of secondary cartilage. We found that Dlx5 is a candidate target gene for miR-200a by microarray analysis. Reporter assay revealed that miR-200a inhibited the promoter activities of Dlx-5. Further, miR-200a reduced the production of Dlx5 protein in ATDC5 cells. Thus, it has been suggested that miR-200a regulates the differentiation of mandibular condylar cartilage cells through inhibition of Dlx5 production.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation.2016
Author(s)
Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
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Journal Title
PLoS One
Volume: 11
Pages: 0152206-0152206
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] A novel measurement method for the morphology of the mandibular ramus using homologous modelling2015
Author(s)
Inoue K, Nakano H, Sumida T, Yamada T, Otawa N, Fukuda N, Nakajima Y, Kumamaru W, Mishima K, Kouchi M, Takahashi I, Mori Y.
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Journal Title
Dentomaxillofac Radiol
Volume: 44
Pages: 20150062-20150062
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Compressive force-produced CCN2 induces osteocyte apoptosis through ERK1⁄2 pathway.2014
Author(s)
K Hoshi, H Kawaki, I Takahashi, N Takeshita, M Seiryu, S A Murshid, T Masuda, T Anada, R Kato, H Kitaura, O Suzuki, T Takano-Yamamoto
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Journal Title
Journal of Bone and Mineral Research
Volume: 29
Pages: 1244-1257
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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