Sirt3 might be a key molecule linking circadian rhythm to aging

• Project/Area Number: 25702005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Eating habits
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Oike Hideaki 国立研究開発法人農業・食品産業技術総合研究機構, その他部局等, 主任研究員 (30455307)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 老化 / 概日リズム / エネルギー代謝 / 栄養 / 体内時計 / 乳酸菌 / 加齢性難聴 / 時計遺伝子 / マウス / Sirt3 / アセチル化 / NAD+

Outline of Final Research Achievements

We found that a chronic shift in light/dark (LD) cycles induced obesity without increasing food intake in mice. Mice fed at a fixed 12 h under the LD shift did not develop symptoms of obesity despite isocaloric intake. These results suggest that disruption of circadian food intake induces obesity.
We demonstrated that intake of Lactococcus lactis subsp. cremoris H61 for 6 months prevented age-related hearing loss in C57BL/6J mice. Analysis of plasma revealed correlation between fatty acids and hearing ability, leading to a novel biomarker for aging.

Research Products

• [Journal Article] Dietary intake of heat-killed Lactococcus lactis H61 delays age-related hearing loss in C57BL/6J mice. (2016)
  • Author(s): Hideaki Oike, Ayako Aoki-Yoshida, Hiromi Kimoto-Nira, Naoko Yamagishi, Satpri Tomita, Yasuyo Sekiyama, Manabu Wakagi, Mutsumi Sakurai, Katsunari Ippoushi, Chise Suzuki, Masuko Kobori
  • Journal Title: Scientific Reports Volume: 6 Issue: 1
  • DOI: 10.1038/srep23556
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Time-fixed feeding prevents obesity induced by chronic advances of light/dark cycles in mouse models of jet-lag/shift work (2015)
  • Author(s): Hideaki Oike, Mutsumi Sakurai, Katsunari Ippoushi, Masuko Kobori
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 465 Issue: 3 Pages: 556-561
  • DOI: 10.1016/j.bbrc.2015.08.059
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 食品成分による老化予防効果の評価 ～マウス加齢性難聴を利用した評価系～ (2015)
  • Author(s): 大池秀明
  • Journal Title: 食品と容器 Volume: 3 Pages: 187-191
  • NAID: 40020379917
• [Journal Article] Nutrients, Clock Genes, and Chrononutrition (2014)
  • Author(s): Oike H, Oishi K, Kobori M
  • Journal Title: Current Nutrition Reports Volume: 3 Issue: 3 Pages: 204-212
  • DOI: 10.1007/s13668-014-0082-6
  • Peer Reviewed / Open Access
• [Presentation] 体内時計を利用してマウスに肥満を誘導する飼育法 (2016)
  • Author(s): 大池秀明、桜井睦、一法師克成、小堀真珠子
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-03-27
• [Presentation] Time-fixed feeding prevents obesity induced by chronic jet lag condition in mouse (2015)
  • Author(s): 大池秀明、一法師克成、小堀真珠子
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-11-21
• [Presentation] マウスモデルを使用した時差ボケ肥満の食餌時刻固定による解消 (2015)
  • Author(s): 大池秀明、桜井睦、一法師克成、小堀真珠子
  • Organizer: 第2回時間栄養科学研究会
  • Place of Presentation: 早稲田大学国際会議場（東京都新宿区）
  • Year and Date: 2015-09-02
• [Presentation] Time-fixed feeding prevents body weight gain induced by chronic jet lag in mice (2015)
  • Author(s): Hideaki Oike, Katsunari Ippoushi, Masuko Kobori
  • Organizer: European Biological Rhythms Society (EBRS)/World Congress of Chronobiology (WCC) meeting
  • Place of Presentation: マンチェスター （イギリス）
  • Year and Date: 2015-08-02
  • Int'l Joint Research
• [Presentation] 食品・栄養成分による体内時計の制御 (2015)
  • Author(s): 大池秀明
  • Organizer: 第１回時間栄養科学研究会
  • Place of Presentation: 早稲田大学（東京都新宿区）
  • Year and Date: 2015-02-14
  • Invited
• [Presentation] マウスにおけるアマニリグナンの概日リズムへ与える影響 (2014)
  • Author(s): 山内優輝、大池秀明、間和彦、小堀真珠子
  • Organizer: 第19回JSoFF2014
  • Place of Presentation: 鹿児島大学（鹿児島県鹿児島市）
  • Year and Date: 2014-11-08
• [Presentation] Characterization of differential response to feeding cues in mouse peripheral clocks (2014)
  • Author(s): Hideaki Oike, Katsunari Ippoushi, Masuko Kobori
  • Organizer: 第21回日本時間生物学会学術大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2014-11-08
• [Presentation] Effect of flaxseed lignan on circadian rhythm in mice (2014)
  • Author(s): Yuki Yamauchi, Hideaki Oike, Kazuhiko Aida, Masuko Kobori
  • Organizer: Sapporo Symposium on Biological Rhythm
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2014-07-26
• [Remarks]
  • URL: http://www.naro.affrc.go.jp/nfri/introduction/chart/0304/hideaki_oike.html