The functional control of thalamostriatal circuit and the understand the mechanism of animal learning behavior

• Project/Area Number: 25702053
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Basic / Social brain science
• Research Institution: Fukushima Medical University
• Principal Investigator: Kato Shigeki 福島県立医科大学, 医学部, 講師 (90443879)
• Co-Investigator(Renkei-kenkyūsha): SANO HIROMI 生理学研究所, その他の部局等, 助教 (00363755) ; FUKABORI RYOJI 福島県立医科大学, 医学部, 助教 (40457784)
• Research Collaborator: SUGAWARA MASATERU 福島県立医科大学, 医学部, 技術補佐 ; KANEMATSU MAKIKO 福島県立医科大学, 医学部, 技術補佐 ; SAWADA NAGISA 福島県立医科大学, 医学部, 技術補佐
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 行動柔軟性 / 実行機能 / 視床線条体路 / 特定神経路機能操作 / 特定神経回路機能 / 逆行性ベクター / 大脳基底核 / 記憶・学習 / 視床線条体経路 / 神経回路 / 逆行性ウイルスベクター / 逆行性レンチウイルスベクター

Outline of Final Research Achievements

Although associative learning happens during learning processes associated with novel stimuli through trial and error, it still remains unclear. We addressed the roles of the pathways originating from central lateral nucleus (CL) in the intralaminar thalamic nuclei in mice. The CL-originated neuronal attenuation did not alter the acquisition of visual discrimination, whereas it disturbed transiently the selection accuracy and lengthened the response time. In addition, the mice impaired the reversal of response discrimination and the set shifting from visual discrimination to response discrimination. Our data suggest that the thalamostriatal systems are involved in cognitive function of basal ganglia circuitry, and that these two circuits possess distinct roles in the control of behavioral selection and flexibility. Furthermore, the subcircuits of basal ganglia including the thalamic nuclear groups that mediate discriminative learning appear to shift during the processes of learning.

Research Products

• [Int'l Joint Research] MIT/Emory University/University of Kansas Medical Center(米国)
• [Int'l Joint Research] Navarra University(スペイン)
• [Int'l Joint Research] San Raffaele Scientific Institute(イタリア)
• [Int'l Joint Research] MIT/Albert Einstein College of Medicine(米国)
• [Int'l Joint Research] University of Navarra(Spain)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] MIT/Albert Einstein College of Medicine(米国)
• [Int'l Joint Research] University of Navarra(Spain)
• [Int'l Joint Research] University of Basel(Switzerland)
• [Journal Article] Action Selection and Flexible Switching Controlled by the Intralaminar Thalamic Neurons (2018)
  • Author(s): Kato Shigeki、Fukabori Ryoji、Nishizawa Kayo、Okada Kana、Yoshioka Nozomu、Sugawara Masateru、Maejima Yuko、Shimomura Kenju、Okamoto Masahiro、Eifuku Satoshi、Kobayashi Kazuto
  • Journal Title: Cell Reports Volume: 22 Issue: 9 Pages: 2370-2382
  • DOI: 10.1016/j.celrep.2018.02.016
  • Peer Reviewed / Open Access
• [Journal Article] Copy number elevation of 22q11.2 genes arrests the developmental maturation of working memory capacity and adult hippocampal neurogenesis. (2018)
  • Author(s): Boku, S., Izumi, T., Abe, S., Takahashi, T., Nishi, A., Nomaru, H., Naka, Y., Kang, G., Nagashima, M., Hishimoto, A., Enomoto, S., Duran-Torres, G., Tanigaki, K., Zhang, J., Ye, K., Kato, S., Mannisto, PT., Kobayashi, K. and Hiroi, N.
  • Journal Title: Mol. Psychiatry Volume: 23 Pages: 985-992
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Gene therapy approaches in the non-human primate model of Parkinson’s disease. (2018)
  • Author(s): Pignataro, D., Sucunza, D., Rico, A., Dopeso-Reyes, I., Roda, E., Rodriguez-Perez, A., Labandeira-Garcia, J., Broccoli, V., Kato. S., Kobayashi, K. and Lanciego, J.
  • Journal Title: J Neural Transm. Volume: 125 Pages: 575-589
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Purkinje cells in the mouse cerebellar vermis directly project their axons to medial parabrachial nucleus. (2017)
  • Author(s): Hashimoto, M., Yamanaka, A., Tanifuji, M., Yaginuma, H.
  • Journal Title: Front Neural Circuit, Volume: 12 Pages: 00006-00006
  • DOI: 10.3389/fncir.2018.00006
  • Peer Reviewed / Open Access
• [Journal Article] Genetic manipulation of specific neural circuits by use of a viral vector system. (2017)
  • Author(s): Kobayashi, K., Kato, S., and Kobayashi, K.
  • Journal Title: J. Neural Transm. Volume: in press Issue: 1 Pages: 67-75
  • DOI: 10.1007/s00702-016-1674-7
  • Peer Reviewed / Open Access
• [Journal Article] Segregated Excitatory?Inhibitory Recurrent Subnetworks in Layer 5 of the Rat Frontal Cortex (2017)
  • Author(s): Morishima Mieko、Kobayashi Kenta、Kato Shigeki、Kobayashi Kazuto、Kawaguchi Yasuo
  • Journal Title: Cerebral Cortex Volume: 27 Issue: 12 Pages: 5846-5857
  • DOI: 10.1093/cercor/bhx276
  • Peer Reviewed / Open Access
• [Journal Article] Pseudotyped lentiviral vectors for retrograde gene delivery into target brain regions. (2017)
  • Author(s): Kobayashi K; Inoue K; Tanabe S; Kato S; Takada M; Kobayashi K
  • Journal Title: Front Neuroanat Volume: 11 Pages: 65-65
  • DOI: 10.3389/fnana.2017.00065
  • Peer Reviewed / Open Access
• [Journal Article] Neuronal cytoskeletal gene dysregulation and mechanical hypersensitivity in a rat model of Rett Syndrome. (2017)
  • Author(s): Bhattacherjee, A., Mu, Y., Winter, M., Knapp, J., Eggiman, L., Gunewardena, S., Kobayashi, K., Kato, S., Krizsan-Agbas, D. and Smith, P.
  • Journal Title: PNAS Volume: 114 Issue: 33
  • DOI: 10.1073/pnas.1618210114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The use of an optimized chimeric envelope glycoprotein enhances the efficiency of retrograde gene transfer of a pseudotyped lentiviral vector in the primate brain. (2017)
  • Author(s): Tanabe S, Inoue K, Tsuge H, Uezono S, Nagaya K, Fijiwara M, Kato S, Kobayashi K, Takada M
  • Journal Title: Neurosci Res Volume: 印刷中 Pages: 45-52
  • DOI: 10.1016/j.neures.2017.02.007
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gene therapy approaches in the non-human primate model of Parkinson’s disease. (2017)
  • Author(s): Pignataro, D., Sucunza, D., Rico, A.J., Dopeso-Reyes, I.G., Roda, E., Rodríguez-Perez, A.I., Labandeira-Garcia, J.L., Broccoli, V., Kato,S., Kobayashi, K., and Lanciego, J.L.
  • Journal Title: J. Neural Transm. Volume: in press Issue: 3 Pages: 575-589
  • DOI: 10.1007/s00702-017-1681-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 4. Involvement of mesolimbic dopaminergic network in neuropathic pain relief by treadmill exercise: A study for specific neural control with Gi-DREADD in mice. (2016)
  • Author(s): Wakaizumi, K., Kondo, T., Hamada, Y., Narita, M., Kawabe, R., Narita, H., Watanabe, M., Kato, S., Senba, E., Kobayashi, K., Kuzumaki, N., Yamanaka, A., Morisaki, H. and Narita, M.
  • Journal Title: Mol. Pain. Volume: 12 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Survival of corticostriatal neurons by Rho/Rho-kinase signaling pathway. (2016)
  • Author(s): Kobayashi K, Sano H, Kato S, Kuroda K, Nakamuta S, Isa T, Nambu A, Kaibuchi K, Kobayashi K.
  • Journal Title: Neurosci Lett. Volume: 630 Pages: 45-52
  • DOI: 10.1016/j.neulet.2016.07.020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Altering Entry Site Preference of Lentiviral Vectors into Neuronal Cells by Pseudotyping with Envelope Glycoproteins. (2016)
  • Author(s): Kobayashi K, Kato S, Inoue K, Takada M, Kobayashi K.
  • Journal Title: Methods Mol Biol. Volume: 1382: Pages: 175-86
  • DOI: 10.1007/978-1-4939-3271-9_12
  • ISBN: 9781493932702, 9781493932719
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Avian sarcoma leukosis virus receptor-envelope system for simultaneous dissection of multiple neural circuits in mammalian brain. (2015)
  • Author(s): Matsuyama, M., Ohashi, Y., Tsubota, T., Yaguchi, M., Kato, S., Kobayashi, K. and Miyashita, Y.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 112 Issue: 22 Pages: 2947-2956
  • DOI: 10.1073/pnas.1423963112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Double Virus Vector Infection to the Prefrontal Network of the Macaque Brain (2015)
  • Author(s): Oguchi M, Okajima M, Tanaka S, Koizumi M, Kikusui T, Ichihara N, Kato S, Kobayashi K, and Sakagami M.
  • Journal Title: PLoS ONE Volume: 10(7) Issue: 7 Pages: e0132825-e0132825
  • DOI: 10.1371/journal.pone.0132825
  • Peer Reviewed / Open Access
• [Journal Article] Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method (2014)
  • Author(s): Watakabe, A., Takaji, M., Kato, S., Kobayashi, K., Mizukami, H., Ozawa, K., Ohsawa, S., Matsui, R., Watanabe, D., and Yamamori, T.
  • Journal Title: Frontiers in neural circuits Volume: 8 Pages: 110-110
  • DOI: 10.3389/fncir.2014.00110
  • Peer Reviewed / Open Access
• [Journal Article] Improved transduction efficiency of a lentiviral vector for neuron-specific retrograde gene transfer by optimizing the junction of fusion envelope glycoprotein. (2014)
  • Author(s): Kato, S., Kobayashi, K., and Kobayashi, K.
  • Journal Title: J Neurosci. Methods Volume: 227 Pages: 151-158
  • DOI: 10.1016/j.jneumeth.2014.02.015
  • Peer Reviewed
• [Journal Article] Viral vector-mediated selective and reversible blockade of the pathway for visual orienting in mice. (2013)
  • Author(s): Sooksawate T, Isa K, Matsui R, Kato S, Kinoshita M, Kobayashi K, Watanabe D, Kobayashi K, Isa T.
  • Journal Title: Frontiers in Neural Circuits Volume: 7 Pages: 162-162
  • DOI: 10.3389/fncir.2013.00162
  • Peer Reviewed
• [Journal Article] Highly efficient retrograde gene transfer into motor neurons by lentiviral vector pseudotyped with fusion glycoprotein. (2013)
  • Author(s): Hirano et al.
  • Journal Title: PLos One Volume: 8 Issue: 9 Pages: e75896-e75896
  • DOI: 10.1371/journal.pone.0075896
  • Peer Reviewed
• [Journal Article] Elucidating information processing in primate basal ganglia circuitry: a novel technique for pathway-selective ablation mediated by immunotoxin. (2013)
  • Author(s): Takada et al.
  • Journal Title: Front. Neural Circuits Volume: 7 Pages: 140-140
  • DOI: 10.3389/fncir.2013.00140
  • Peer Reviewed
• [Presentation] 特定神経回路の機能制御を可能とする逆行性ウイルスベクターの開発 (2017)
  • Author(s): 加藤 成樹、井上 謙一、高田 昌彦、小林 和人
  • Organizer: 平成28年度 京都大学霊長類研究所共同利用研究会
  • Place of Presentation: 京都大学霊長類研究所 大会議室
  • Year and Date: 2017-03-18
  • Invited
• [Presentation] Behavioral selection and flexible switching through thalamostriatal circuit. (2017)
  • Author(s): Shigeki KATO
  • Organizer: International Workshop on Basal Ganglia Functions 2017
  • Int'l Joint Research / Invited
• [Presentation] Thalamostriatal circuit controls action selection and flexible switching in learning behavior. (2017)
  • Author(s): Shigeki KATO
  • Organizer: 平成29年度第2回大脳基底核機能研究会
  • Invited
• [Presentation] 行動選択および行動柔軟性における視床-線条体路の機能解析 (2017)
  • Author(s): 加藤 成樹, 小林 和人
  • Organizer: 第40回日本分子生物学会
• [Presentation] Optimizing the junction of chimeric fusion glycoprotein of a lentiviral vector for highly neuron-specific retrograde gene transfer. (2017)
  • Author(s): Shigeki KATO, Masateru Sugawara and Kazuto KOBAYASHI
  • Organizer: 第40回日本神経科学大会
• [Presentation] Pathway-selective manipulation of neural function by using HiRet/NeuRet vector system. (2016)
  • Author(s): Shigeki KATO
  • Organizer: The International Workshop for Strategy for Neuroscience
  • Place of Presentation: Convention Center, National Institute of Physiological Science, Okazaki, Japan
  • Year and Date: 2016-12-08
  • Int'l Joint Research / Invited
• [Presentation] Comparative study of gene transduction efficiency for retrograde gene transfer lentiviral vector with novel fusion envelope glycoproteins. (2016)
  • Author(s): Shigeki KATO and Kazuto KOBAYASHI
  • Organizer: Neuroscience 2016
  • Place of Presentation: SanDiego.CA.USA・San Diego Convention Center
  • Year and Date: 2016-11-16
  • Int'l Joint Research
• [Presentation] 特定神経回路を標的とした機能制御システムの開発と応用 (2016)
  • Author(s): 加藤 成樹、菅原 正晃、小林 和人
  • Organizer: 第7回福島運動器疼痛セミナー
  • Place of Presentation: ホテルサンルートプラザ福島
  • Year and Date: 2016-11-05
  • Invited
• [Presentation] 線条体Striosome/Matrixシステムの行動機能的意義の解析 (2016)
  • Author(s): 加藤 成樹
  • Organizer: 福島県立医科大学・Molecular Medicine Seminar
  • Place of Presentation: 福島県立医科大学10号館会議室
  • Year and Date: 2016-10-26
  • Invited
• [Presentation] Comparative study of gene transduction efficiency for retrograde gene transfer lentiviral vector with novel fusion envelope glycoproteins (2016)
  • Author(s): Kato, S. and Kobayashi, K.
  • Organizer: International Symposium on Adaptive Circuit Shift 2016 (ACS2016)
  • Place of Presentation: Kyoto, Japan・Kambaikan, Doshisha University
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Thalamostriatal system controls selection and flexible switching of behavior (2016)
  • Author(s): Kobayashi, K. and Kato, S.
  • Organizer: International Symposium on Adaptive Circuit Shift 2016 (ACS2016)
  • Place of Presentation: Kyoto, Japan・Kambaikan, Doshisha University
  • Year and Date: 2016-03-03
  • Int'l Joint Research / Invited
• [Presentation] Optimizing the junction of fusion glycoprotein for the transduction efficiency of a lentiviral vector for neuron-specific retrograde gene transfer. (2014)
  • Author(s): Shigeki KATO, Kenta Kobayashi and Kazuto KOBAYASHI
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2014-09-12
• [Presentation] 高頻度逆行性ベクターによる大脳ー小脳ー基底核ネットワークへの遺伝子導入
  • Author(s): 加藤 成樹, 菅原 正晃, 小林 和人
  • Organizer: 平成25年度 領域ミーティング(CREST)
  • Place of Presentation: 東京・JST東京本部別館1階ホール
• [Presentation] 刺激弁別学習における2種類の主要な視床線条体路の行動生理学的役割/Behavioral and physiological roles of two major thalamostriatal pathways in sensory discrimination learning.
  • Author(s): 加藤 成樹, 深堀良二, 小林 和人
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸・神戸ポートアイランド
• [Presentation] Functional control of specific neural pathways in basal ganglia circuit by HiRet.
  • Author(s): Shigeki KATO, Ryoji FUKABORI, Kenta Kobayashi, Hiromu Yawo, Yoshikazu Isomura and Kazuto KOBAYASHI
  • Organizer: Neuroscience 2013
  • Place of Presentation: SanDiego.CA.USA・San Diego Convention Center
• [Presentation] Comparative analysis for behavioral roles of the central lateral and parafascicular nuclear groups projecting to the dorsolateral striatum.
  • Author(s): Shigeki KATO, Ryoji FUKABORI and Kazuto KOBAYASHI
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 京都・京都国際会館
• [Book] Gene Therapy (2013)
  • Author(s): Kato, S., Kobayashi, K., Inoue, K., Takada, M., and Kobayashi, K.
  • Total Pages: 12
  • Publisher: InTech