Start-up of "Cell Thermodynamics": Studies on thermal properties and signaling in single living cells.

• Project/Area Number: 25707035
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Jikei University School of Medicine (2015); Waseda University
• Principal Investigator: Oyama Kotaro 東京慈恵会医科大学, 医学部, 特別研究員 (70632131)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
• Keywords: 細胞熱力学 / 温度測定 / 蛍光イメージング / 熱物性 / 温度感受性 / 神経細胞 / ブレブ / 熱伝導 / 単一細胞 / 熱伝導率 / 細胞熱物性

Outline of Final Research Achievements

How does single living cell generate, sense, and efficiently use local changes in temperature? To answer the question, here we started the research area on “Cell Thermodynamics”. In this study, we analyzed heat transfer in single living cells. We also clarified temperature-sensitivities in various cells, such as directional bleb formation of cancer cells under local temperature gradient and neurite outgrowth triggered by microheating. These results provided us valuable information on thermal signaling at single-cell level.

Research Products

• [Int'l Joint Research] Russian Academy of Sciences(ロシア連邦)
• [Journal Article] Directional bleb formation in spherical cells under temperature gradient (2015)
  • Author(s): Oyama, K.,* Arai, T., Isaka, A., Sekiguchi, T., Itoh, H., Seto, Y., Miyazaki, M., Itabashi, T., Ohki, T., Suzuki, M.* and Ishiwata, S.* (Corresponding authors)
  • Journal Title: Biophys. J. Volume: 109 Issue: 2 Pages: 355-364
  • DOI: 10.1016/j.bpj.2015.06.016
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Triggering of high-speed neurite outgrowth using an optical microheater. (2015)
  • Author(s): Kotaro Oyama, Vadim Zeeb, Yuki Kawamura, Tomomi Arai, Mizuho Gotoh, Hideki Itoh, Takeshi Itabashi, Madoka Suzuki & Shin’ichi Ishiwata
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 16611-16611
  • DOI: 10.1038/srep16611
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The 105 gap issue between calculation and measurement in single-cell thermometry (2015)
  • Author(s): Madoka Suzuki, Vadim Zeeb, Satoshi Arai, Kotaro Oyama, and Shin’ichi Ishiwata
  • Journal Title: Nature Methods Volume: 12 Pages: 802-803
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat (2015)
  • Author(s): Shintani, S., Oyama, K. Fukuda, N., and Ishiwata, S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 457 Issue: 2 Pages: 165-170
  • DOI: 10.1016/j.bbrc.2014.12.077
  • Peer Reviewed
• [Journal Article] Microscopic heat pulse-induced calcium dynamics in single WI-38 fibroblasts (2014)
  • Author(s): Itoh, H., Oyama, K., Suzuki M., and Ishiwata, S.
  • Journal Title: BIOPHYSICS Volume: 10 Issue: 0 Pages: 109-119
  • DOI: 10.2142/biophysics.10.109
  • NAID: 130004940803
  • ISSN: 1349-2942
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescent Nanothermometers for Local Temperature Measurement Inside Living Cells (2014)
  • Author(s): Kotaro Oyama, Satoshi Arai, Madoka Suzuki
  • Journal Title: The Bulletin of the Society of Nano Science and Technology Volume: 13(1) Pages: 49-56
  • NAID: 40020260511
• [Presentation] Regulation of cellular functions by microscopic heat pulses (2015)
  • Author(s): Kotaro Oyama, Madoka Suzuki, Shin’ichi Ishiwata
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] Thermosensitive Ca2+ burst in rat hippocampal neurons through ryanodine receptors (2015)
  • Author(s): Yuki Kawamura, Kotaro Oyama, Hideki Itoh, Vadim Zeeb, Madoka Suzuki, Shin’ichi Ishiwata
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-09-13
• [Presentation] High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of rat (2015)
  • Author(s): Seine A Shintani, Kotaro Oyama, Norio Fukuda, Shin'ichi Ishiwata
  • Organizer: 第120回 日本解剖学会総会･全国学術集会・第92回 日本生理学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] CHARACTERIZATION OF α-TROPOMYOSIN (TM) MUTANTS THAT CAUSE HYPERTROPHIC CARDIOMYOPATHY (HCM) IN HUMANS: IN VITRO MOTILITY ASSAYS WITH A MICROSCOPIC HEAT PULSE (2015)
  • Author(s): Shuya Ishii, Kotaro Oyama, Madoka Suzuki, Masataka Kawai, Shin’ichi Ishiwata
  • Organizer: Biophysical Society 59th Annual Meeting
  • Place of Presentation: Boltimore, California
  • Year and Date: 2015-02-07 – 2015-02-11
• [Presentation] 温度勾配によって誘起されるHeLa細胞のブレブ形成 (2015)
  • Author(s): 大山廣太郎、新井智実、井阪智、関口卓、伊藤秀城、瀬戸裕介、宮崎牧人、板橋岳志、大木高志、鈴木団、石渡信一
  • Organizer: 2015年生体運動合同班会議
  • Place of Presentation: 東京
  • Year and Date: 2015-01-07 – 2015-01-09
• [Presentation] 心筋細胞内における昇温誘起高速サルコメア振動 (2015)
  • Author(s): 新谷正嶺、大山廣太郎、福田紀男、石渡信一
  • Organizer: 2015年生体運動合同班会議
  • Place of Presentation: 東京
  • Year and Date: 2015-01-07 – 2015-01-09
• [Presentation] Characterization of tropomyosin mutants that cause hypertrophic cardiomyopathy (HCM): In vitro assays with optical tweezers and heat pulse (2014)
  • Author(s): Shuya Ishii, Kotaro Oyama, Madoka Suzuki, Masataka Kawai, Shin'ichi Ishiwata
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] High-frequency sarcomeric auto-oscillations in living cardiomyocytes under hyperthermal conditions (2014)
  • Author(s): Seine Shintani, Kotaro Oyama, Norio Fukuda, Shin’ichi Ishiwata
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Ca2+-burst in rat hippocampal neurons induced by microscopic heat pulses (2014)
  • Author(s): Yuki Kawamura, Kotaro Oyama, Hideki Itoh, Madoka Suzuki, Shin’ichi Ishiwata
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 北海道
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 細胞内の温度測定に適した蛍光ナノ温度計 (2014)
  • Author(s): 大山廣太郎，高林昌生、武井義明、村田篤、新井敏、武岡真司、石渡信一、鈴木団
  • Organizer: 第12回ナノ学会大会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-22 – 2014-05-24
• [Presentation] 局所熱パルス法を用いて細胞の温度感受性を解明する (2014)
  • Author(s): 大山廣太郎、新谷正嶺、伊藤秀城、石井秀弥、福田紀男、鈴木団、石渡信一
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] THERMAL ACTIVATION OF CARDIAC THIN FILAMENTS INDUCES CONTRACTION WITHOUT INTRACELLULAR CA2+ CHANGES: STUDIES WITH CARDIOMYOCYTES AND AN IN VITRO MOTILITY ASSAY (2014)
  • Author(s): Kotaro Oyama, Shuya Ishii, Tomomi Arai, Seine A. Shintani, Hideki Itoh, Norio Fukuda, Madoka Suzuki, Shin'ichi Ishiwata.
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco
• [Presentation] BLEB FORMATION IN A HELA CELL INDUCED BY TEMPERATURE GRADIENT. (2014)
  • Author(s): Tomomi Arai, Akira Isaka, Kotaro Oyama, Hideki Itoh, Yusuke Seto, Madoka Suzuki, Shin'ichi Ishiwata.
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco
• [Presentation] Mapping of thermal conductivity in single living cells (2013)
  • Author(s): Taku Sekiguchi, Kotaro Oyama, Hideki Itoh, Madoka Suzuki, Shin’ichi Ishiwata.
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 京都
• [Presentation] Calcium release induced by a microscopic heat pulse in WI-38 fibroblasts (2013)
  • Author(s): Hideki Itoh, Kotaro Oyama, Madoka Suzuki, Shin'ichi Ishiwata
  • Organizer: The 5th EMBO meeting
  • Place of Presentation: Amsterdam
• [Book] CRC Concise Encyclopedia of Nanotechnology (2015)
  • Author(s): Madoka Suzuki , Satoshi Arai , Kotaro Oyama , Shin’ichi Ishiwata
  • Total Pages: 8
  • Publisher: CRC Press