Effect of high-energy visible light on animal cells.

• Project/Area Number: 25740018
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Tamagawa University
• Principal Investigator: SATO Kazuomi 玉川大学, 農学部, 准教授 (10554991)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: アポトーシス / 細胞死 / 可視光線 / 青色光 / ミトコンドリア / メラニン生成 / メラノーマ / ミトコンドリア膜電位 / 活性酸素 / ネクローシス

Outline of Final Research Achievements

The aim of the present work was to clarify the mechanism of cell death induced by visible light. Blue light induced mitochondrial membrane potential depolarization subsequent to elevation of intracellular superoxide production. Moreover, blue light induced cell surface exposure of phosphatidylserine. Mitochondrial membrane potential is an indicator of mitochondria-dependent apoptosis. Thus, blue light causes mitochondrial dysfunction and subsequent apoptosis.

Research Products

• [Journal Article] Effect of monochromatic visible light on intracellular superoxide anion production and mitochondrial membrane potential of B16F1 and B16F10 murine melanoma cells. (2013)
  • Author(s): Kazuomi Sato, Yuji Minai, Hiroyuki Watanabe
  • Journal Title: Cell Biology International Volume: 37 Issue: 6 Pages: 633-637
  • DOI: 10.1002/cbin.10069
  • Peer Reviewed
• [Presentation] 可視単色光によるアポトーシス誘導メカニズムの解明 (2015)
  • Author(s): 中村咲貴、西尾貴史、寺田千里、薬袋裕二、渡辺博之、佐藤一臣
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2015-03-27
• [Presentation] ブルーライトによるメラニン生成促進効果の検討 (2015)
  • Author(s): 西尾貴史、佐藤一臣
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 2015-03-27
• [Presentation] メラニンから人類の歴史と今後について考える (2015)
  • Author(s): 佐藤一臣
  • Organizer: 第19回相模農薬セミナー
  • Place of Presentation: 相模中央化学研究所(神奈川県綾瀬市)
  • Year and Date: 2015-01-08 – 2015-01-09
  • Invited
• [Presentation] The mechanism of blue light-induced cell death in melanoma cells. (2014)
  • Author(s): Kazuomi Sato, Takashi Nishio, Takuma Mito, Katsuki Matsui, Junko N. Sato, Hiroyuki Watanabe
  • Organizer: International Pigment Cell Conference 2014
  • Place of Presentation: Shangri-La hotel Singapore(Singapore)
  • Year and Date: 2014-09-04 – 2014-09-06
• [Presentation] 青色光による細胞死誘導メカニズムの解明
  • Author(s): 佐藤一臣、三戸拓磨、松井克樹、杉本幸浩、薬袋裕二、渡邊博之
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市総合体育館(熊本市)
• [Presentation] アセチルサリチル酸によるメラニン生成抑制メカニズムの解明
  • Author(s): 西尾貴史、宇佐美舞、淡路瑞樹、篠原すみれ、鳥山優、佐藤一臣
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本市総合体育館(熊本市)