What is the molecular mechanism of stress fiber disassembly caused by cyclic stretch?

• Project/Area Number: 25750154
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: HUANG Wenjing 九州工業大学, 若手研究者フロンティア研究アカデミー, 研究員 (00633413)
• Research Collaborator: DEGUCHI Shinji 名古屋工業大学, おもひ領域, 准教授 (30379713) ; MATSUI Tsubasa 名古屋工業大学, おもひ領域, 特任助教 (50638707)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Cyclic stretch / Stress fiber disassembly / Nonmuscle myosin II / stress fiber disassembly / lengthening phase / shortening phase / real-time observation / Actin stress fiber / Disassembly / nonmuscle myosin II

Outline of Final Research Achievements

Stress fibers (SFs), which consist of actin filaments and nonmuscle myosin II (NMII), undergo disassembly after cyclic stretch (CS) exposure. CS involves cell lengthening and shortening. I found that fast shorteningof cells that exceeded the intrinsic contraction speed of SFs, but not slow shortening and fast/slow lengthening, caused SF disassembly. Since NMII disassociation from actin occurred in cells exposed to a fast shortening process exceeding the rate of the NMII motion, the results of the study demonstrated that NMII should be involved in the disassembly of SFs in cells, which was further confirmed by basic experiments: SFs whose actin-NMII interactions were restricted were more prone to disassembly upon fast cell shortening. On the other hand, SF disassembly still occurred in cells exposed to CS even in cofilin-inactivated cells. Therefore, the results demonstrate that dissociation of NMII from actin bundles is the primary mechanism for CS-induced SF disassembly.

Research Products

• [Journal Article] Egg-in-Cube: Design and Fabrication of a Novel Artificial Eggshell with Functionalized Surface (2015)
  • Author(s): Wenjing Huang, Fumihito Arai, and Tomohiro Kawahara
  • Journal Title: PLOS ONE Volume: 10(3) Issue: 3 Pages: e0118624-e0118624
  • DOI: 10.1371/journal.pone.0118624
  • Peer Reviewed / Open Access
• [Journal Article] Sensitivity and Dose Denpendency of Radiation-induced injury in hematopoietic Stem/Progenitor Cells in Mice (2015)
  • Author(s): Chang-Ying Guo, Lan Luo, Yoshishige Urata, Shinji Goto, Wen-Jing Huang, Syu Takamura, Fumiko Hayashi, Hanako Doi, Yuriko Kitajima, Yusuke Ono, Tomoo Ogi, and Tao-Sheng Li
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 8055-8055
  • DOI: 10.1038/srep08055
  • Peer Reviewed / Open Access
• [Journal Article] Promotion of Migration and Extension of Human Umbilical Vein Endothelial Cells by Readily Soluble Calcium Phosphate (2014)
  • Author(s): Wenjing Huang, Masaaki Abe, Toshitake Furusawa, Yuta Matsushima, and Hidero Unuma
  • Journal Title: Journal of Japanese Society of Oral Implantology Volume: 27 Issue: 3 Pages: 331-336
  • DOI: 10.11237/jsoi.27.331
  • NAID: 130004709637
  • ISSN: 0914-6695, 2187-9117
  • Peer Reviewed
• [Journal Article] Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells (2014)
  • Author(s): Lan Luo, Miho Kawakatsu, Chao-Wan Guo, Yoshishige Urata, Wen-Jing Huang, Haytham Ali, Hanako Doi, Yuriko Kitajima, Takayuki Tanaka, Shinji Goto, Yusuke Ono, Hong-Bo Xin, Kimikazu Hamano, and Tao-Sheng Li
  • Journal Title: Scientific Reports Volume: 4 Issue: 1 Pages: 1-7
  • DOI: 10.1038/srep03779
  • Peer Reviewed / Open Access
• [Presentation] ストレスファイバーの方向依存脱重合のメカニズムに関する研究 (2015)
  • Author(s): 黄 文敬, 松井 翼, 倉賀野 正弘, 高橋 正行, 川原 知洋, 佐藤 正明, 出口 真次
  • Organizer: 日本機械学会 第２７回バイオエンジニアリング講演会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] 繰り返し伸展刺激を受ける細胞の観察プラットホームの実現 (2015)
  • Author(s): 黄文敬, Ahmad Belal, 川原知洋
  • Organizer: 日本機械学会 第２７回バイオエンジニアリング講演会
  • Place of Presentation: 朱鷺メッセ新潟コンベンションセンター
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] 繰り返し伸展刺激によって起こるストレスファイバーの脱重合のメカニズムについて (2014)
  • Author(s): 黄 文敬, 松井 翼, 倉賀野 正弘, 高橋 正行, 川原 知洋, 佐藤 正明, 出口 真次
  • Organizer: 第５２回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] A system for real-time imaging of living cells exposed to cyclic stretch (2014)
  • Author(s): Wenjing Huang, Belal Ahmad, and Tomohiro Kawahara
  • Organizer: 4th Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: Shima Kanko Hotel The Classic, Shima, Mie, Japan
  • Year and Date: 2014-09-01 – 2014-09-04
• [Presentation] 力方向依存的なストレスファイバー脱重合のメカニズムに関する研究 (2014)
  • Author(s): 黄 文敬，松井 翼，佐藤 正明，出口 真次
  • Organizer: 第26回バイオエンジニアリング講演会
  • Place of Presentation: 東北大学（仙台市青葉区片平二丁目１－１）
• [Presentation] Design and Fabrication of Cubic Eggshell for a Novel Biomedical Platform using Chick Embryo (2014)
  • Author(s): Wenjing Huang, Fumihito Arai, and Tomohiro Kawahara
  • Organizer: IEEE International Conference on Biomedical Robotics and Biomechatronics
  • Place of Presentation: Anhembi Convention Center, Sao Paulo, Brazil
• [Patent(Industrial Property Rights)] 細胞状態観察・計測装置 (2014)
  • Inventor(s): 川原 知洋, 黄 文敬, Ahmad Belal
  • Industrial Property Rights Holder: 川原 知洋, 黄 文敬, Ahmad Belal
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-095917
  • Filing Date: 2014-05-07