Development of microfluidic device on antibody immobilized-thermoresponsive surfaces for cell sorting

Research Project

Project/Area Number	25750179
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Biomedical engineering/Biomaterial science and engineering
Research Institution	Tokyo Women's Medical University
Principal Investigator	KOBAYASHI Jun 東京女子医科大学, 医学部, 講師 (20385404)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	生体材料 / 再生医療 / 細胞分離 / 抗体 / マイクロ流体
Outline of Final Research Achievements	Antibody-immobilized thermoresponsive surfaces were designed for achieving selective attachment and detachment of cells accompanied by temperature-dependent conformational changes of grafted thermoresponsive polymer. At 37 °C, anti-human CD90 antibody-immobilized thermoresponsive surfaces selectively held CD90-positive Ty-82 cells in the mixture of Ty-82 cells and CD90-negative K562 cells. Adhered Ty-82 cells were detached by both lowering temperature and applying shear stress, resulting in the enrichment of Ty-82 cells. More precise separation and purification of cells would be achieved by controlling the flow of shear stress within microfluidic channels.