| Project/Area Number |
25750179
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KOBAYASHI Jun 東京女子医科大学, 医学部, 講師 (20385404)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生体材料 / 再生医療 / 細胞分離 / 抗体 / マイクロ流体 |
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| Outline of Final Research Achievements |
Antibody-immobilized thermoresponsive surfaces were designed for achieving selective attachment and detachment of cells accompanied by temperature-dependent conformational changes of grafted thermoresponsive polymer. At 37 °C, anti-human CD90 antibody-immobilized thermoresponsive surfaces selectively held CD90-positive Ty-82 cells in the mixture of Ty-82 cells and CD90-negative K562 cells. Adhered Ty-82 cells were detached by both lowering temperature and applying shear stress, resulting in the enrichment of Ty-82 cells. More precise separation and purification of cells would be achieved by controlling the flow of shear stress within microfluidic channels.
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