| Project/Area Number |
25750386
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
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| Research Institution | Keio University |
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Principal Investigator |
OHNO Osamu 慶應義塾大学, 理工学部, 助教 (20436992)
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| Research Collaborator |
SUENAGA Kiyotake
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | biselyngbyaside / biselyngbyolide / kurahyne / シアノバクテリア / アポトーシス / 小胞体ストレス / カルシウム / biselyngbyaside類 / biselyngbyolide類 / SERCA / biselyngbyolide B / アフィニティー精製 / tomuruline / 血清飢餓 |
|---|
| Outline of Final Research Achievements |
New biselyngbyaside analogs, biselyngbyolides B and C and biselyngbyasides E and F, were isolated from the marine cyanobacterium Lyngbya sp. Their structures were determined by spectroscopic analyses. These compounds inhibited the growth of human cancer cells, and biselyngbyolides B and C showed stronger activity than other analogs. Based on the detailed analyses of their biological activities, biselyngbyolides B and C were revealed to show potent endoplasmic reticulum (ER) stress-inducing activity. A new lipopeptide, kurahyne, was isolated from a cyanobacterial assemblage of Lyngbya sp. Kurahyne also exhibited ER stress-inducing activity. By the analyses using fluorescent and biotin conjugates of kurahyne, it was likely that kurahyne localized in ER and bound to ER Ca2+ pump.
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