| Project/Area Number |
25750396
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
FUTAMURA Yushi 独立行政法人理化学研究所, 長田抗生物質研究室, 訪問研究員 (70525857)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | モルフォベース / ケミカルバイオロジー / バイオプローブ / ポストゲノム創薬 |
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| Outline of Final Research Achievements |
To discover small drug like molecules, we have carried out the phenotypic screening based on MorphoBase, an encyclopedia of cellular morphology, consisting of the cell-shape changes induced by various compounds. Among over 10000 compounds deposited in NPDepo library, we found that one eudesman compound show unique cell-shape changes in tsNRK cells and G2/M phase arrest in HeLa cells. Immunofluorescence experiments revealed that this compound induces cell cycle arrest at metaphase without any effect on the spindle formation and chromatin segregation, indicating that it has a distinct mode of action from typical antimitotic drugs.
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