The role of the terminal domains of Bombyx mori silk fibroin in the fiber formation process
| Project/Area Number |
25810124
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer/Textile materials
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| Research Institution | University of Fukui (2014-2016)
Tokyo University of Agriculture and Technology (2013) |
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Principal Investigator |
Suzuki Yu 福井大学, テニュアトラック推進本部, 講師 (90600263)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 絹フィブロイン / 組換えタンパク質 / 大腸菌 / 液状絹 / ＮＭＲ / 繊維化メカニズム / 繊維化プロセス / NMR |
|---|
| Outline of Final Research Achievements |
The aim of this study was investigating the role of the terminal domains of Bombyx mori silk fibroin in the fiber formation process. For structure determination of the terminal domains, we worked on the expression system construction of the silk fibroin N-terminal domain. The pET28a plasmid vector containing the DNA sequence of N-terminal domain was constructed successfully. Then, this plasmid vector was introduced into the E.coli competent cell DH5a and the plasmid was amplified. After that, this plasmid was introduced into the E.coli BL21(DE3) which was the competent cell for protein expression and cultured but the N-terminal domain protein was not expressed.
Currently, we are working on the protein expression by using the other kind of competent cells as well as the cell-free expression system.
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Structural Determination of the Tandem Repeat Motif in Samia cynthia ricini Liquid Silk by Solution NMR2015
Author(s)
Suzuki, Y, Kawanishi, S., Yamazaki, T., Aoki, A., Saito, H., Asakura, T.
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Journal Title
Macromolecules
Volume: 48
Issue: 18
Pages: 6574-6579
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Biological Reaction to Small-Diameter Vascular Grafts Made of Silk Fibroin Implanted in the Abdominal Aortae of Rats2015
Author(s)
Fukayama, T., Takagi, K., Tanaka, R., Hatakeyama, Y., Aytemiz, D., Suzuki, Y., Asakura, T.
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Journal Title
Annals of Vascular Surgery
Volume: 29
Issue: 2
Pages: 341-352
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Structural Transition of Bombyx mori Liquid Silk Studied with Vibrational Circular Dichroism Spectroscopy2015
Author(s)
Morisaku, T., Arai, T., Konno, K., Suzuki, Y., Asakura, T., Yui, H.
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Journal Title
Analytical Sciences
Volume: in press
NAID
Related Report
Peer Reviewed
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