| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The aim of this study was investigating the role of the terminal domains of Bombyx mori silk fibroin in the fiber formation process. For structure determination of the terminal domains, we worked on the expression system construction of the silk fibroin N-terminal domain. The pET28a plasmid vector containing the DNA sequence of N-terminal domain was constructed successfully. Then, this plasmid vector was introduced into the E.coli competent cell DH5a and the plasmid was amplified. After that, this plasmid was introduced into the E.coli BL21(DE3) which was the competent cell for protein expression and cultured but the N-terminal domain protein was not expressed.
Currently, we are working on the protein expression by using the other kind of competent cells as well as the cell-free expression system.
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