Cell monitoring system for the construction of three-dimensional organ in the tissue engineering

• Project/Area Number: 25820093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Aichi Institute of Technology
• Principal Investigator: Yamada Akira 愛知工業大学, 工学部, 准教授 (20377815)
• Research Collaborator: NARUSE Keiji ; MOHRI Satoshi ; MATSUURA Koji ; TERAMOTO Akira ; MIURA Tsuyoshi ; WAKUDA Akihiko ; NOBUKUNI Masashi ; SASAKI Yuta
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 再生医療 / ISFET / pH / pH-FET / 生分解性樹脂 / 三次元微細構造 / pH/PCO2センサ / カテーテル / 細胞モニタ

Outline of Final Research Achievements

We have developed a monitoring system for the observation and evaluation of the growing condition for the precursor tissue that is transplanted into the patients. We constructed a tissue monitoring system in conjunction with a micro camera and pH/PCO2 sensor. The camera was used for the evaluation of cell morphology. The pH/PCO2 sensor was used for the evaluation of the quantity of respiration of the cell or tissue. During this development, we especially constructed a amplifier for the sensor and a software for PC to control the measurement system. We have also investigated the liquid junction problem which was problematic under the measurement of pH values. The liquid junction produce a voltage that are called a liquid junction potential and cause a measurement error. We have investigated to this point by constructing a special device based on microfluidics.

Research Products

• [Journal Article] A miniaturized microfluidic pH measurement device using ISFET sensors (2016)
  • Author(s): Yuta Sasaki, Masashi Nobukuni, Akira Yamada
  • Journal Title: 2016 IEEE International Symposium on Micro-NanoMechatronics and Human Science Volume: - Pages: 128-130
  • Peer Reviewed
• [Journal Article] Expansion of a linear range of the ion-concentration measurement system using microfluidic device with ISFET sensors (2015)
  • Author(s): Masashi Nobukuni, Akihiko Wakuda, Tsuyoshi Miura, Akira Yamada
  • Journal Title: 2015 IEEE International Symposium on Micro-NanoMechatronics and Human Science (MHS) Volume: - Pages: 80-81
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Simple Method for Decreasing the Liquid Junction Potential in a Flow-through-Type Differential pH Sensor Probe Consisting of pH-FETs by Exerting Spatiotemporal Control of the Liquid Junction (2015)
  • Author(s): Akira Yamada, Satoshi Mohri, Michihiro Nakamura, Keiji Naruse
  • Journal Title: Sensors Volume: 15 Issue: 4 Pages: 7898-7912
  • DOI: 10.3390/s150407898
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A simple ion concentration measurement system using a microfluidic device with ISFET sensors (2014)
  • Author(s): Tsuyoshi Miura, Akira Yamad
  • Journal Title: 2014 IEEE International Symposium on Micro-NanoMechatronics and Human Science Volume: - Pages: 103-106
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A simple estimation method of photosynthetic condition based on pH measurement using ISFET sensor (2013)
  • Author(s): Akira Teramoto, Akira Yamada
  • Journal Title: 2013 IEEE International Symposium on Micro-NanoMechatronics and Human Science Volume: - Pages: 158-160
  • Peer Reviewed
• [Presentation] 微小流体デバイスとISFETセンサを用いたインライン濃度モニタ (2016)
  • Author(s): 三浦 剛、山田 章
  • Organizer: 第２５回日本コンピュータ外科学会大会特集号
  • Place of Presentation: 東京工科大学
  • Year and Date: 2016-11-26
• [Presentation] 微小流体デバイスと半導体イオンセンサによる高精度pH測定法の開発 (2016)
  • Author(s): 信國真史、佐々木優太、山田 章
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-06-08
• [Presentation] 微小流体デバイスによるイオン濃度モニタの超小型化 (2016)
  • Author(s): 佐々木優太、信國真史、山田 章
  • Organizer: 第55回日本生体医工学会
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2016-04-26
• [Presentation] 佐々木優太、信國真史、山田 章 (2016)
  • Author(s): 半導体センサISFETを用いた微小流体デバイスの開発
  • Organizer: 日本機械学会東海支部第65期総会・講演会
  • Place of Presentation: 愛知工業大学
  • Year and Date: 2016-03-17
• [Presentation] 微小流体デバイスによるイオン濃度モニタの線形範囲の拡大 (2015)
  • Author(s): 和久田暁彦、山田 章
  • Organizer: 第５４回日本生体医工学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-05-07 – 2015-05-09
• [Presentation] 半導体センサISFETを用いた微小流体デバイスの開発 (2015)
  • Author(s): 和久田 暁彦、山田 章
  • Organizer: 日本機械学会東海支部 第46回学生会卒業研究発表講演会
  • Place of Presentation: 中部大学
  • Year and Date: 2015-03-12
• [Presentation] 微小流体デバイスによるインラインイオン濃度モニタ (2014)
  • Author(s): 三浦 剛資、山田 章
  • Organizer: 第53回日本生体医工学会大会
  • Place of Presentation: 仙台国際センター
• [Presentation] 水草の光合成活性測定システム ―光強度依存性の簡便な評価手法の確立― (2013)
  • Author(s): 寺本 晃、山田 章
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会
  • Place of Presentation: つくば国際会議場
• [Patent(Industrial Property Rights)] ｐＨ測定デバイス (2015)
  • Inventor(s): 山田 章
  • Industrial Property Rights Holder: 山田 章
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-249994
  • Filing Date: 2015-12-22