Evaluation of usability of IL1RAPL1 knockout mice as an animal model of neuropsychiatric disorders

• Project/Area Number: 25830048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Yamanashi
• Principal Investigator: YASUMURA Misato 山梨大学, 総合研究部, 助教 (20533897)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 行動解析 / シナプスオーガナイザー / 知的障害 / 自閉症 / IL1RAPL1 / シナプス形成 / 行動テストバッテリー

Outline of Final Research Achievements

The spine density of basal dendrites of cortical layer 2/3 and hippocampal CA1 pyramidal neurons was significantly reduced in IL1RAPL1 knockout mice. We found that the spatial reference and working memories and remote fear memory were impaired in IL1RAPL1 knockout mice. These results suggest that IL1RAPL1 knockout mice are an animal model of intellectual disability. IL1RAPL1 knockout mice also exhibited slightly reduced behavioural flexibility and increased sociability. In addition, IL1RAPL1 knockout mice exhibited high locomotor activity and reduced open-space and height anxiety-like behaviours.

Research Products

• [Journal Article] Phenotypic analysis of IL1RAPL1 knockout mice (2015)
  • Author(s): 安村美里, 吉田知之, 三品昌美
  • Journal Title: Folia Pharmacologica Japonica Volume: 145 Issue: 4 Pages: 187-192
  • DOI: 10.1254/fpj.145.187
  • NAID: 130005061554
  • ISSN: 0015-5691, 1347-8397
• [Journal Article] IL1RAPL1 knockout mice show learning deficiency, memory decline, hyperactivity and reduced anxiety-like behaviours. (2014)
  • Author(s): Misato Yasumura, Tomoyuki Yoshida, Maya Yamazaki, Manabu Abe, Rie Natsume, Kouta Kanno, Takeshi Uemura, Keizo Takao, Kenji Sakimura, Takefumi Kikusui, Tsuyoshi Miyakawa, Masayoshi Mishina
  • Journal Title: Scientific Reports Volume: 4: 6613 Issue: 1 Pages: 6613-6613
  • DOI: 10.1038/srep06613
  • Peer Reviewed / Open Access
• [Presentation] IL1RAPL1欠損マウスの行動学的解析
  • Author(s): 安村美里、吉田知之、高雄啓三、山崎真弥、阿部学、植村健、宮川剛、﨑村建司、三品昌美
  • Organizer: 第36回日本神経科学大会
  • Place of Presentation: 国立京都国際会館(京都府京都市)
• [Presentation] IL1RAPL1遺伝子欠損マウスの表現型解析
  • Author(s): 安村美里、三品昌美
  • Organizer: 第８７回日本薬理学会年会
  • Place of Presentation: 仙台国際センター(宮城県仙台市)
• [Book] Cortical development (Chapter 11 Synapse formation in the brain) (2013)
  • Author(s): Masayoshi Mishina, Tomoyuki Yoshida, Misato Yasumura and Takeshi Uemura (eds. Ryuichiro Kageyama, Tetsuo Yamamori)
  • Total Pages: 19
  • Publisher: Springer
• [Remarks] research map
  • URL: http://researchmap.jp/3310/