Expression and function of Siglec-H in the nervous system

• Project/Area Number: 25830050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University
• Principal Investigator: Konishi Hiroyuki 名古屋大学, 医学(系)研究科(研究院), 助教 (90448746)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ミクログリア / マクロファージ / マーカー / 炎症 / グリア / 神経損傷 / 神経再生 / 抗体 / モノクローナル抗体

Outline of Final Research Achievements

We examined the possibility that sialic acid binding Ig-like lectin (Siglec)-H was a novel microglial marker. We revealed that Siglec-H was expressed in microglia but not macrophages and furthermore that Siglec-H locus is useful for microglia-specific gene targeting using Siglec-H knock-in mouse. We also performed functional analysis of Siglec-H-deficient mouse and found that Siglec-H might suppress inflammatory responses of microglia after neuronal injury.

Research Products

• [Journal Article] Increase of transcription factor EB (TFEB) and lysosomes in rat DRG neurons and their transportation to the central nerve terminal in dorsal horn after nerve injury (2016)
  • Author(s): Jung J, Uesugi N, Jeong NY, Park BS, Konishi H, Kiyama H
  • Journal Title: Neuroscience Volume: 313 Pages: 10-22
  • DOI: 10.1016/j.neuroscience.2015.11.028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ontogeny and innervation of taste buds in mouse palatal gustatory epithelium (2016)
  • Author(s): Rashwan A, Konishi H, El-Sharaby A, Kiyama H
  • Journal Title: J Chem Neuroanat Volume: 71 Pages: 26-40
  • DOI: 10.1016/j.jchemneu.2015.11.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A DAP12-Dependent Signal Promotes Pro-Inflammatory Polarization in Microglia Following Nerve Injury and Exacerbates Degeneration of Injured Neurons. (2015)
  • Author(s): Kobayashi M, Konishi H, Takai T, Kiyama H.
  • Journal Title: Glia Volume: 63 Issue: 6 Pages: 1073-1082
  • DOI: 10.1002/glia.22802
  • Peer Reviewed / Open Access
• [Journal Article] N-terminal Cleaved Pancreatitis-associated Protein-III (PAP-III) Serves as a Scaffold for Neurites and Promotes Neurite Outgrowth. (2013)
  • Author(s): H. Konishi, S. Mateumoto, K. Namikawa, H. Kiyama
  • Journal Title: The Journal of Biological Chemistry Volume: 288(15) Issue: 15 Pages: 10205-10213
  • DOI: 10.1074/jbc.m112.395301
  • Peer Reviewed
• [Journal Article] Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine (2013)
  • Author(s): Kyohei Tokizane
  • Journal Title: Molecular and Cellular Endocrinology Volume: 372 Issue: 1-2 Pages: 4956-4956
  • DOI: 10.1016/j.mce.2013.03.012
  • Peer Reviewed
• [Journal Article] Dysfunction in the hypothalamo- hypophyseal system under chronic stress and fatigue (2013)
  • Author(s): Ogawa T., Konishi H., Kiyama H
  • Journal Title: Advance in Neuroimmune Biology Volume: 4 Issue: 4 Pages: 219-228
  • DOI: 10.3233/nib-130070
  • Peer Reviewed
• [Presentation] 6-sialyl-lewisc on n-glycan may be involved in microglial phagocytosis of neuron (2015)
  • Author(s): Handa M, Yoshimura T, Torii T, Konishi H, Kiyama H, Ikenaka K
  • Organizer: 25th Biennial Meeting of the International Society for Neurochemistry
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2015-08-24
  • Int'l Joint Research
• [Presentation] Augmentation of neuropathic pain by DAP12 mediated signal in microglia (2015)
  • Author(s): Konishi H, Kobayashi M, Takai T, Kiyama H
  • Organizer: XII European Meeting on Glial Cell Function in Health and Disease
  • Place of Presentation: Bilbao, Spain
  • Year and Date: 2015-07-15
  • Int'l Joint Research
• [Presentation] A DAP12-dependent signal promotes pro-Inflammatory polarization in microglia following nerve injury and exacerbates degeneration of injured neurons (2015)
  • Author(s): Kobayashi M, Konishi H, Takai T, Kiyama H
  • Organizer: XII European Meeting on Glial Cell Function in Health and Disease
  • Place of Presentation: Bilbao, Spain
  • Year and Date: 2015-07-15
  • Int'l Joint Research
• [Presentation] ミクログリアが形成する脳内環境と損傷運動ニューロンの運命 (2015)
  • Author(s): 小西博之、小林正明、木山博資
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 神経損傷後のミクログリア活性化制御因子 (2014)
  • Author(s): 小西博之、小林正明、木山博資
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ミクログリアDAP12による炎症反応は神経損傷後の神経細胞死を増悪させる (2014)
  • Author(s): 小林正明、小西博之、高井俊行、木山博資
  • Organizer: 第37回日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] DAP12を介した神経損傷後のミクログリア活性化 (2014)
  • Author(s): 小西博之、小林正明、高井俊行、木山博資
  • Organizer: 第119回日本解剖学会総会全国学術総会
  • Place of Presentation: 自治医科大学（下野市）
• [Presentation] 持続的ストレス負荷により下垂体のメラノトロフに発現するVGFとその発現制御 (2013)
  • Author(s): 時實恭平、 小西博之、 安井正佐也、 小川登紀子、 佐々木一樹、 南野直人、 木山博資
  • Organizer: 第56回日本神経化学会大会
  • Place of Presentation: 京都国際会館（京都市）
• [Presentation] ミクログリアの抗炎症作用機序-神経損傷反応性に発現するSiglec-Hについて (2013)
  • Author(s): 小林正明、小西博之、木山博資
  • Organizer: 第56回日本神経化学会大会
  • Place of Presentation: 京都国際会館（京都市）
• [Remarks] 所属講座
  • URL: http://www.med.nagoya-u.ac.jp/Anatomy2
• [Remarks] 所属講座
  • URL: http://www.med.nagoya-u.ac.jp/Anatomy2