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Functional analysis of novel miRNA and molecule regulating immune responses.

Research Project

Project/Area Number 25830062
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionNagasaki University (2014-2015)
Tokyo University of Science (2013)

Principal Investigator

YONEZAWA, Tomo  長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (60515964)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsmiRNA / 新規分子 / 炎症応答
Outline of Final Research Achievements

We found miRNAs and novel molecules regulating inflammatory responses. To examine whether or not novel molecule, TRIM39R regulates immune responses, we established two gain-of-function models in cultured cells. Then, we performed microarray analysis on the models. The results indicates that TRIM39R significantly regulates genes involved in type I interferon pathway and its anti-viral effects. Furthermore, we confirmed that it actually induces immune responses, such as NFkB and IRF, using luciferase-based reporter assay. We also demonstrated novel miRNA involved in immune responses, mir-125b regulates its target, ADAMTS-4 mRNA according to miRNA dogma by luciferase-based assay and site-directed mutagenesis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Int'l Joint Research] School of Chemistry/Wuhan University of Technology(China)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] TNF-α induces caspase-1 activation independently of simultaneously induced NLRP3 in 3T3-L1 cells.2016

    • Author(s)
      Furuoka M, Ozaki K, Sadatomi D, Mamiya S, Yonezawa T, Tanimura S, Takeda K.
    • Journal Title

      J. Cell Physiol.

      Volume: in press Issue: 12 Pages: 1-7

    • DOI

      10.1002/jcp.25385

    • NAID

      120006987671

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Supplementation of long-chain fatty acids with lactating mammary glands and beef cow has potential benefits.2015

    • Author(s)
      Riho Kurata, Xiaofeng Cui, Roh Sang-gun, Tomo Yonezawa
    • Journal Title

      Advances in plants and agriculture research

      Volume: 2 Issue: 5 Pages: 00063-00063

    • DOI

      10.15406/apar.2015.02.00063

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TRIM39, but not TRIM39B, regulates type I interferon response.2013

    • Author(s)
      Riho Kurata, Atsushi Tajima, Tomo Yonezawa and Hidetoshi Inoko
    • Journal Title

      Biochemcal and Biophysical Research Communications

      Volume: 436 Issue: 1 Pages: 90-95

    • DOI

      10.1016/j.bbrc.2013.05.064

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Free fatty acids-sensing G protein-coupled receptors in drug targeting and therapeutics2013

    • Author(s)
      Tomo Yonezawa, Riho Kurata, Kaori Yoshida, Masanori A Murayama, Xiaofeng Cui and Akihiko Hasegawa
    • Journal Title

      Current Medicinal Chemistry

      Volume: 20 Issue: 31 Pages: 3855-3871

    • DOI

      10.2174/09298673113209990168

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] MicroRNA-125b regulates the expression of aggrecanase-1 (ADAMTS-4) in human osteoarthritic chondrocytes.2013

    • Author(s)
      Matsukawa T, Sakai T, Yonezawa T, Hiraiwa H, Hamada T, Nakashima M, Ono Y, Ishizuka S, Nakahara H, Lotz MK, Asahara H, Ishiguro N.
    • Journal Title

      Arthritis Res Ther.

      Volume: 15(1) Issue: 1

    • DOI

      10.1186/ar4164

    • NAID

      40020134052

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Pronuclear injection of circular plasmids expressing hCas9 and gRNA generates mutant mice in genes involved in immune responses.2014

    • Author(s)
      Tomo Yonezawa, Akihisa Oda, Riho Kurata, Sachiko Kubo, Shyuhei Ogawa, Yoichiro Iwakura
    • Organizer
      The 43rd Annual Meeting of The Japanese Society for Immunology.
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] Pronuclear injection of circular plasmids expressing hCas9 and gRNA generates mutant mice in genes involved in immune responses.2014

    • Author(s)
      Tomo Yonezawa, Akihisa Oda, Riho Kurata, Sachiko Kubo, Shyuhei Ogawa, Yoichiro Iwakura
    • Organizer
      International Cytokine & Interferon Society (ICIS)-Cytokines Down Under in 2014: From Bench to Beyond
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2014-10-26 – 2014-10-29
    • Related Report
      2014 Research-status Report
  • [Presentation] TRIM39, but not TRIM39B, regulates type I interferon response.2013

    • Author(s)
      Riho Kurata, Atsushi Tajima, Tomo Yonezawa, Hidetoshi Inok
    • Organizer
      15th International Congress of Immunology
    • Place of Presentation
      Milan, Italy
    • Related Report
      2013 Research-status Report
  • [Presentation] Functional analysis for microRNA involving in arthritis.2013

    • Author(s)
      Tomo Yonezawa
    • Organizer
      15th International Congress of Immunology
    • Place of Presentation
      Milan, Italy
    • Related Report
      2013 Research-status Report
  • [Presentation] TRIM39R, but not TRIM39B regulates type I interferon2013

    • Author(s)
      倉田 里穗、田嶋 敦、米沢 朋、猪子 英俊
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Presentation] Transcriptomic analysis of miRNA in differentiation of T cell subsets2013

    • Author(s)
      Tomo Yonezawa
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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