| Project/Area Number |
25830062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagasaki University (2014-2015)
Tokyo University of Science (2013) |
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Principal Investigator |
YONEZAWA, Tomo 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (60515964)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | miRNA / 新規分子 / 炎症応答 |
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| Outline of Final Research Achievements |
We found miRNAs and novel molecules regulating inflammatory responses. To examine whether or not novel molecule, TRIM39R regulates immune responses, we established two gain-of-function models in cultured cells. Then, we performed microarray analysis on the models. The results indicates that TRIM39R significantly regulates genes involved in type I interferon pathway and its anti-viral effects. Furthermore, we confirmed that it actually induces immune responses, such as NFkB and IRF, using luciferase-based reporter assay. We also demonstrated novel miRNA involved in immune responses, mir-125b regulates its target, ADAMTS-4 mRNA according to miRNA dogma by luciferase-based assay and site-directed mutagenesis.
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