Functional analysis of novel miRNA and molecule regulating immune responses.

• Project/Area Number: 25830062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Nagasaki University (2014-2015); Tokyo University of Science (2013)
• Principal Investigator: YONEZAWA, Tomo 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (60515964)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: miRNA / 新規分子 / 炎症応答

Outline of Final Research Achievements

We found miRNAs and novel molecules regulating inflammatory responses. To examine whether or not novel molecule, TRIM39R regulates immune responses, we established two gain-of-function models in cultured cells. Then, we performed microarray analysis on the models. The results indicates that TRIM39R significantly regulates genes involved in type I interferon pathway and its anti-viral effects. Furthermore, we confirmed that it actually induces immune responses, such as NFkB and IRF, using luciferase-based reporter assay. We also demonstrated novel miRNA involved in immune responses, mir-125b regulates its target, ADAMTS-4 mRNA according to miRNA dogma by luciferase-based assay and site-directed mutagenesis.

Research Products

• [Int'l Joint Research] School of Chemistry/Wuhan University of Technology(China)
• [Journal Article] TNF-α induces caspase-1 activation independently of simultaneously induced NLRP3 in 3T3-L1 cells. (2016)
  • Author(s): Furuoka M, Ozaki K, Sadatomi D, Mamiya S, Yonezawa T, Tanimura S, Takeda K.
  • Journal Title: J. Cell Physiol. Volume: in press Issue: 12 Pages: 1-7
  • DOI: 10.1002/jcp.25385
  • NAID: 120006987671
  • Peer Reviewed
• [Journal Article] Supplementation of long-chain fatty acids with lactating mammary glands and beef cow has potential benefits. (2015)
  • Author(s): Riho Kurata, Xiaofeng Cui, Roh Sang-gun, Tomo Yonezawa
  • Journal Title: Advances in plants and agriculture research Volume: 2 Issue: 5 Pages: 00063-00063
  • DOI: 10.15406/apar.2015.02.00063
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TRIM39, but not TRIM39B, regulates type I interferon response. (2013)
  • Author(s): Riho Kurata, Atsushi Tajima, Tomo Yonezawa and Hidetoshi Inoko
  • Journal Title: Biochemcal and Biophysical Research Communications Volume: 436 Issue: 1 Pages: 90-95
  • DOI: 10.1016/j.bbrc.2013.05.064
  • Peer Reviewed
• [Journal Article] Free fatty acids-sensing G protein-coupled receptors in drug targeting and therapeutics (2013)
  • Author(s): Tomo Yonezawa, Riho Kurata, Kaori Yoshida, Masanori A Murayama, Xiaofeng Cui and Akihiko Hasegawa
  • Journal Title: Current Medicinal Chemistry Volume: 20 Issue: 31 Pages: 3855-3871
  • DOI: 10.2174/09298673113209990168
  • Peer Reviewed
• [Journal Article] MicroRNA-125b regulates the expression of aggrecanase-1 (ADAMTS-4) in human osteoarthritic chondrocytes. (2013)
  • Author(s): Matsukawa T, Sakai T, Yonezawa T, Hiraiwa H, Hamada T, Nakashima M, Ono Y, Ishizuka S, Nakahara H, Lotz MK, Asahara H, Ishiguro N.
  • Journal Title: Arthritis Res Ther. Volume: 15(1) Issue: 1 Pages: R28-R28
  • DOI: 10.1186/ar4164
  • NAID: 40020134052
  • Peer Reviewed
• [Presentation] Pronuclear injection of circular plasmids expressing hCas9 and gRNA generates mutant mice in genes involved in immune responses. (2014)
  • Author(s): Tomo Yonezawa, Akihisa Oda, Riho Kurata, Sachiko Kubo, Shyuhei Ogawa, Yoichiro Iwakura
  • Organizer: The 43rd Annual Meeting of The Japanese Society for Immunology.
  • Place of Presentation: 京都国際会議場（京都府）
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Pronuclear injection of circular plasmids expressing hCas9 and gRNA generates mutant mice in genes involved in immune responses. (2014)
  • Author(s): Tomo Yonezawa, Akihisa Oda, Riho Kurata, Sachiko Kubo, Shyuhei Ogawa, Yoichiro Iwakura
  • Organizer: International Cytokine & Interferon Society (ICIS)-Cytokines Down Under in 2014: From Bench to Beyond
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2014-10-26 – 2014-10-29
• [Presentation] TRIM39, but not TRIM39B, regulates type I interferon response. (2013)
  • Author(s): Riho Kurata, Atsushi Tajima, Tomo Yonezawa, Hidetoshi Inok
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Milan, Italy
• [Presentation] Functional analysis for microRNA involving in arthritis. (2013)
  • Author(s): Tomo Yonezawa
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: Milan, Italy
• [Presentation] TRIM39R, but not TRIM39B regulates type I interferon (2013)
  • Author(s): 倉田 里穗、田嶋 敦、米沢 朋、猪子 英俊
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Transcriptomic analysis of miRNA in differentiation of T cell subsets (2013)
  • Author(s): Tomo Yonezawa
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸