Differentiation and transplantation of insulin-secreting cells generated from mouse-derived iPS cells.

• Project/Area Number: 25830064
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Tokyo Medical University
• Principal Investigator: KUMAGAI Katsuyoshi 東京医科大学, 医学部, 助教 (20567911)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: マウスES/iPS細胞 / インスリン産生細胞 / 再生医療 / マウスES細胞 / マウスiPS細胞 / Gck

Outline of Final Research Achievements

Islet transplantation has been suggested to be a promising treatment for type 1 and type 2 diabetes. iPS cells have potential cure for diabetes using a cellular therapy to ameliorate symptoms associated with both reduced insulin secretion and insulin sensitivity. Therefore, highly efficient differentiation of iPS cells into insulin-secreting cells will enhance the potential for patient specific cell transplantation therapy in diabetes. Here, we demonstrate that mouse-derived iPS cells can be differentiated into insulin-secreting cells in vitro and that they respond to glucose stimulation under physiological conditions. The hyperglycemic phenotype in model mice was improved by transplantation of the insulin-secreting cells.