| Project/Area Number |
25830081
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ヒストン脱メチル化酵素 / Fbxl10 / トランスジェニックマウス / 造血幹細胞 |
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| Outline of Final Research Achievements |
I found that overexpression of Fbxl10, a histone demethylase, in hematopoietic stem cells induced leukemia. To elucidate the underlying molecular mechanisms, I isolated Fbxl10-overexpressing hematopoietic stem cell and performed RNA-seq analysis. I found the enhanced expression patterns of neuron specific gene 2 (Nsg2) that is implicated in intracellular transport, and genes involved in oxidative phosphorylation. In addition, by ChIP analysis, I confirmed that these genes are direct targets of Fbxl10. These results provide novel insights into the leukemogenic mechanisms from the viewpoint of intracellular transport and oxidative phosphorylation.
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