Identification and functional analysis of phosphotyrosine proteins in scirrhous gastric cancer
| Project/Area Number |
25830096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Shirakihara Takuya 国立研究開発法人国立がん研究センター, 研究所, 研究員 (30548756)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | スキルス胃がん / チロシンリン酸化 / 受容体型チロシンキナーゼ / リン酸化チロシン / FGF受容体 / Met / 癌微小環境 |
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| Outline of Final Research Achievements |
We aimed to identify some novel phosphotyrosine proteins related to development of Scirrhours gastric cancer. Although coculture of gastric cancer cells with surrounding cells was performed, none of apparent phosphorylation depend on coculture were detected. So next we tried to reveal downstream effectors of receptor tyrosine kinases such as Met or FGFR2 in diffused type gastric cancers. Proteins purified using anti-receptor antibody and anti-phosphotyrosine antibody, then identified by mass spectrometry. Finally, some data of functional analysis indicates these identified proteins have important roles in malignancy of Scirrhous gastric cancer.
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Report
(4 results)
Research Products
(8 results)
