Identification and functional analysis of phosphotyrosine proteins in scirrhous gastric cancer

• Project/Area Number: 25830096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: National Cancer Center Japan
• Principal Investigator: Shirakihara Takuya 国立研究開発法人国立がん研究センター, 研究所, 研究員 (30548756)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: スキルス胃がん / チロシンリン酸化 / 受容体型チロシンキナーゼ / リン酸化チロシン / FGF受容体 / Met / 癌微小環境

Outline of Final Research Achievements

We aimed to identify some novel phosphotyrosine proteins related to development of Scirrhours gastric cancer. Although coculture of gastric cancer cells with surrounding cells was performed, none of apparent phosphorylation depend on coculture were detected. So next we tried to reveal downstream effectors of receptor tyrosine kinases such as Met or FGFR2 in diffused type gastric cancers. Proteins purified using anti-receptor antibody and anti-phosphotyrosine antibody, then identified by mass spectrometry. Finally, some data of functional analysis indicates these identified proteins have important roles in malignancy of Scirrhous gastric cancer.

Research Products

• [Journal Article] Identification of integrin α3 as a molecular marker of cells undergoing epithelial-mesenchymal transition and of cancer cells with aggressive phenotypes. (2013)
  • Author(s): Shirakihara T, Kawasaki T, Fukagawa A, Semba K, Sakai R, Miyazono K, Miyazawa K, Saitoh M.
  • Journal Title: Cancer Science Volume: 104 Issue: 9 Pages: 1189-1197
  • DOI: 10.1111/cas.12220
  • Peer Reviewed
• [Presentation] スキルス胃がんにおける活性化受容体結合タンパク質の同定と機能解析 (2015)
  • Author(s): 白木原琢哉, 山口英樹, 堺隆一
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 愛知県名古屋市名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] スキルス胃がんの浸潤・腹膜播種に関与する活性化受容体結合タンパク質の解析 (2015)
  • Author(s): 白木原琢哉, 山口英樹, 堺隆一
  • Organizer: 第24回日本がん転移学会学術集会・総会
  • Place of Presentation: 大阪府大阪市シティプラザ大阪
  • Year and Date: 2015-07-23
• [Presentation] Involvement of FGF signaling in metastasis and peritoneal dissemination of diffuse gastric cancer (2014)
  • Author(s): 白木原琢哉、堺隆一
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] スキルス胃がんの浸潤・腹膜播種へのFGFシグナルの関与 (2014)
  • Author(s): 白木原琢哉、堺隆一
  • Organizer: 第23回日本がん転移学会学術集会・総会
  • Place of Presentation: 石川県金沢市金沢ニューグランドホテル
  • Year and Date: 2014-07-10 – 2014-07-11
• [Presentation] FGF-2 accelerates TGF-beta-induced epithelial-mesenchymal transition and promotes invasion of cancer (2013)
  • Author(s): Takuya Shirakihara, Sakai Ryuichi, Masao Saitoh
  • Organizer: Mechanismms & Models of Cancer Symposium 2013
  • Place of Presentation: San Diego (USA)
• [Presentation] TGF-beta誘導性EMTの亢進とがん浸潤への関与 (2013)
  • Author(s): 白木原琢哉、齋藤正夫、堺隆一
  • Organizer: 第22回日本がん転移学会学術集会・総会
  • Place of Presentation: 長野県松本市ホテルブエナビスタ
• [Presentation] FGF-2 accelerates TGF-beta-induced epithelial-mesenchymal transition and promotes invasion of cancer (2013)
  • Author(s): 白木原琢哉、堺隆一、齋藤正夫
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜