Paclitaxel resistance-associated proteins in lung cancer cell lines are detected by proteome analyses

• Project/Area Number: 25830104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Shimomura Masanori 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90433268)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: パクリタキセル耐性 / プロテオーム解析 / パクリタキセル耐性関連タンパク質 / パクリタキセル / 抗がん剤耐性 / パクリタキセル固定化ビーズ

Outline of Final Research Achievements

We evaluated the function of paclitaxel resistance-associated proteins detected by proteome analyses in human lung cancer cell lines. Affinity purification by paclitaxel immobilized beads revealed several specific bands in paclitaxel resistant cell line; these bands were not revealed in the other cell lines. We can especially identify a protein, FIGNL1, of these specific proteins. As a result of knockdown of FIGNL1 expression using siRNA, the resistant cell line had survival rate of only 60% on the low concentration paclitaxel.

Research Products

• [Presentation] Paclitaxel resistance is associated with drug accumulation in intracellular compartments and paclitaxel-binding proteins in human lung cancer cell lines (2013)
  • Author(s): M. Shimomura, T. Yaoi, K. Itoh, D. Kato, J. Shimada, S Fushiki
  • Organizer: European Cancer Congress 2013
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] Paclitaxel resistance is associated with drug accumulation in intracellular compartments and paclitaxel-binding proteins in human lung cancer cell lines (2013)
  • Author(s): M. Shimomura, T. Yaoi, K. Itoh, D. Kato, J. Shimada, S Fushiki
  • Organizer: World Conference of Lung Cancer 2013
  • Place of Presentation: Sydney, Australia