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Bacterial genomic DNA protection mechanism against oxidative stresses

Research Project

Project/Area Number 25840002
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Ohniwa Ryosuke  筑波大学, 医学医療系, 准教授 (30447883)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords核様体 / 酸化ストレス / 細菌 / 染色体 / 遺伝学
Outline of Final Research Achievements

The protection of genomic DNA against oxidative stresses is essential to sustain the living cells including symbiotic/pathogenic bacteria inhabiting human. Here, I studied the molecular mechanisms to protect bacterial genomic DNA. I focused on about ten proteins which I have shown to be equipped on the the bacterial nucleoid which is the complex of genomic DNA, protein, etc. Among the proteins examined by techniques of bacterial molecular genetics, Dps protein in an opportunistic pathogen Staphylococcus aureus exhibited that its DNA binding and nucleoid clogging abilities are not sufficient to protect genomic DNA and cells, and that its ferroxidase activity (ferric iron chelating ability) is essential.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) (of which Invited: 1 results)

  • [Int'l Joint Research] National Taiwan University(台湾)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Institute of Pasteur(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] In vitro DNA Protection Assay Using Oxidative Stress2015

    • Author(s)
      Ushijima Y, Ohniwa RL, and Morikawa K.
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oseltamivir Expands Quasispecies of Influenza Virus through Cell-to-cell Transmission2015

    • Author(s)
      Mori K, Murano K, Ohniwa RL, Kawaguchi A, Nagata K.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 9163-9163

    • DOI

      10.1038/srep09163

    • NAID

      120007130320

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Staphylococcus aureus dry stress survivors have a heritable fitness advantage in subsequent dry exposure.2015

    • Author(s)
      Maudsdotter L, Imai S, Ohniwa RL, Saito S, Morikawa K
    • Journal Title

      Microbes and Infection

      Volume: 15 Issue: 6 Pages: 456-461

    • DOI

      10.1016/j.micinf.2015.02.004

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nucleoid compaction by MrgAAsp56Ala/Glu60Ala does not contribute to staphylococcal cell survival against oxidative stress and phagocytic killing by macrophage2014

    • Author(s)
      Yuri Ushijima, Ryosuke L. Ohniwa, Atsushi Maruyama, Shinji Saito, Yoshikazu Tanaka, and Kazuya Morikawa
    • Journal Title

      FEMS Microbiology Letters

      Volume: 360 Issue: 2 Pages: 144-151

    • DOI

      10.1111/1574-6968.12598

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evidence for lateral gene transfer (LGT) in the evolution of eubacteria-derived small GTPases in plant organelles.2014

    • Author(s)
      Suwastika IN, Denawa M, Yomogihara S, Im CH, Bang WY, Ohniwa RL, Bahk JD, Takeyasu K, Shiina T.
    • Journal Title

      Front Plant Sci.

      Volume: 5 Pages: 678-678

    • DOI

      10.3389/fpls.2014.00678

    • NAID

      120007136464

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Atomic force microscopy analysis of the role of major DNA-binding proteins in organization of the nucleoid in Escherichia coli.2013

    • Author(s)
      Ohniwa RL, Muchaku H, Saito S, Wada C and Morikawa K
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 8 Pages: e72954-e72954

    • DOI

      10.1371/journal.pone.0072954

    • NAID

      120007136837

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conservation of two distinct types of 100S ribosome in bacteria.2013

    • Author(s)
      Ueta, M., et al
    • Journal Title

      Genes to Cells

      Volume: 18 Issue: 7 Pages: 554-574

    • DOI

      10.1111/gtc.12057

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] A genomic DNA protection system2013

    • Author(s)
      Ryosuke L. Ohniwa, Yuri Ushijima, Kazuya Morikawa.
    • Organizer
      1st Singapore-Japan-India Joint Symposium on Protein-DNA interactions in Prokaryotic nucleoid and Eukaryotic chromatin
    • Place of Presentation
      National University of Singapore
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Althernative cardiolipin synthase Cls1 compensates for stalled Cls2 function in Staphylococcus aureus under conditions of acute acid stress2013

    • Author(s)
      Ryosuke L. Ohniwa, Kana Kibatayashi, Kazuya Morikawa
    • Organizer
      7th International Conference on Gram-Positive Microorganisims
    • Place of Presentation
      Italy (Tsucany)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2022-02-16  

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