Bacterial genomic DNA protection mechanism against oxidative stresses
| Project/Area Number |
25840002
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 核様体 / 酸化ストレス / 細菌 / 染色体 / 遺伝学 |
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| Outline of Final Research Achievements |
The protection of genomic DNA against oxidative stresses is essential to sustain the living cells including symbiotic/pathogenic bacteria inhabiting human. Here, I studied the molecular mechanisms to protect bacterial genomic DNA. I focused on about ten proteins which I have shown to be equipped on the the bacterial nucleoid which is the complex of genomic DNA, protein, etc. Among the proteins examined by techniques of bacterial molecular genetics, Dps protein in an opportunistic pathogen Staphylococcus aureus exhibited that its DNA binding and nucleoid clogging abilities are not sufficient to protect genomic DNA and cells, and that its ferroxidase activity (ferric iron chelating ability) is essential.
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Report
(4 results)
Research Products
(11 results)
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[Presentation] A genomic DNA protection system2013
Author(s)
Ryosuke L. Ohniwa, Yuri Ushijima, Kazuya Morikawa.
Organizer
1st Singapore-Japan-India Joint Symposium on Protein-DNA interactions in Prokaryotic nucleoid and Eukaryotic chromatin
Place of Presentation
National University of Singapore
Related Report
Invited
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