Regulation of chromatin structure and gene expression in cross-tolerance to environmental stress

• Project/Area Number: 25840007
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Kyoto University
• Principal Investigator: TARUMOTO Yusuke 京都大学, 生命科学研究科, 助教 (70551381)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ストレス応答 / シグナル伝達 / 遺伝子発現制御 / クロマチン / 分子生物学

Outline of Final Research Achievements

We have studied cellular responses to low-dose environmental stress. Cross-tolerance is an adaptive phenomenon, in which exposure to low-dose stress confers transient resistance to other form of high-dose stress. Previously we have identified Cpc2 as an important factor for cross-tolerance. This study revealed that Cpc2 plays a role in the regulation of stress-induced gene expression via facilitating nucleosome remodeling.

Research Products

• [Journal Article] Cellular response to low-dose stress (2014)
  • Author(s): 樽本雄介、石川冬木
  • Journal Title: Leading Author's Volume: 3 Pages: e004
  • DOI: 10.7875/leading.author.3.e004
  • Peer Reviewed / Open Access
• [Journal Article] Receptor for activated C-kinase (RACK1) homolog Cpc2 facilitates the general amino acid control response through Gcn2 kinase in fission yeast (2013)
  • Author(s): Tarumoto, Y. Kanoh, J. Ishikawa, F.
  • Journal Title: The Journal of Biological Chemistry Volume: 288(26) Issue: 26 Pages: 19260-19268
  • DOI: 10.1074/jbc.m112.445270
  • Peer Reviewed / Open Access
• [Presentation] 分裂酵母Stn1の染色体構造維持における役割 (2014)
  • Author(s): 滝川 雅大、樽本 雄介、石川 冬木
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Fission yeast HIRA histone chaperone facilitates stress-induced transcriptional response (2014)
  • Author(s): Yusuke Tarumoto, Moeko Chujo, Koichi Miyatake, Eisuke Nishida, and Fuyuki Ishikawa
  • Organizer: IIAS Research Conference 2014: Chromatin Decoding
  • Place of Presentation: 京都
  • Year and Date: 2014-05-12 – 2014-05-15
• [Presentation] Fission yeast Cpc2 facilitates stress-activated MAP kinase-mediated stress response (2013)
  • Author(s): Yusuke Tarumoto, Junko Kanoh, Toshihide Ueno, Hiroyuki Mano, Fuyuki Ishikawa
  • Organizer: EMBO Conference on Fission Yeast: pombe 2013
  • Place of Presentation: イギリス、ロンドン
• [Presentation] 分裂酵母Cpc2はストレス応答性MAPキナーゼを介したストレス応答を促進する (2013)
  • Author(s): 樽本雄介、加納純子、上野敏秀、間野博行、石川冬木
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜