Nuclear IKKbeta regulates necroptosis and hepatocellular carcinoma

• Project/Area Number: 25840035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Hiroshima University
• Principal Investigator: Tsuchiya Yoshihiro 広島大学, 医歯薬学保健学研究院(医), 助教 (90611301)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 肝障害 / 肝がん / IKK / 肝線維化 / NF-kB

Outline of Final Research Achievements

In this study, we aimed to reveal roles of the cytosolic and nuclear IKKβ in decision of cell life and death in vivo. Tg-NLS-IKKβKN IKKβΔhep mice presented with severe liver injury and fibrosis progression. Administration of diethylnitrosamine (DEN) to Tg-NLS-IKKβKN IKKβΔhep mice shown lower a level of hepatocellular carcinoma than that observed in IKKβΔhep mice.

Research Products

• [Presentation] RelAの転写活性化ドメイン(TAD)を介したIKK活性制御機構 (2015)
  • Author(s): 土谷佳弘、樋口徹、松永泰花、高橋江奈、中津祐介、浅野知一郎、鎌田英明
  • Organizer: BMB2015
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-01
• [Presentation] RelA suppresses IKKb activity in a trascription-independent manner (2014)
  • Author(s): 土谷佳弘, 浅野知一郎, 鎌田英明
  • Organizer: 第87回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] RelAによる遺伝子発現非依存的なIKK活性制御機構 (2014)
  • Author(s): 土谷佳弘, 浅野知一郎, 鎌田英明
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Stress-induced NF-kappaB activation mediated by the nuclear IKKbeta and cell death (2013)
  • Author(s): Yoshihihiro Tsuchiya, Mayu Kanamoto, Masamoto Kanno, Tomoichiro Asano, Hideaki Kamata
  • Organizer: 第８６回 日本生化学大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] RelA associates Hsp70 and suppresses IKKbeta; activity in a trascription-independent manner (2013)
  • Author(s): Yoshihihiro Tsuchiya, Mayu Kanamoto, Masamoto Kanno, Tomoichiro Asano, Hideaki Kamata
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Book] 医学のあゆみ 活性酸素 (2013)
  • Author(s): 土谷佳弘、鎌田英明
  • Total Pages: 988
  • Publisher: 医歯薬出版株式会社