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The mechanism of ubiquitination system in collective cell migration.

Research Project

Project/Area Number 25840068
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionChiba University

Principal Investigator

MIZOGUCHI TAKAMASA  千葉大学, 薬学研究科(研究院), 助教 (10645419)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsMib / p120ctn / Rac1 / 細胞集団移動 / ユビキチン化 / Mib1 / 細胞移動 / Mind bomb
Outline of Final Research Achievements

We found that the posterior lateral line primordium (pLLP) migration was delayed in mind bomb (mib) mutants. Mib is an E3 ubiquitin ligase and a positive regulator of Notch signal. We also found that Mib could potentially control cell migration via Notch signaling independent pathway.
We showed that Mib interacted with and ubiquitinated p120 catenin (p120ctn), which controls cell migration. It is known that p120ctn overexpression induces activation of Rac1. We found that Mib suppressed p120ctn dependent Rac1 activation. These data indicate that Mib controls cell migration via negative regulation of p120ctn activity.
Finally, we tested the hypothesis that exaggerated p120ctn activity in mib mutant causes the delay in pLLP migration. Morpholino knockdown of p120ctn rescued pLLP migration defects in mib mutants. Taken together, our data suggest that Mib is involved in pLLP migration through ubiquitination of p120ctn.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (11 results)

  • [Journal Article] 増大特集 細胞シグナル操作法 Ⅰ.分子からみたシグナル操作法 4.その他 Notchシグナル2015

    • Author(s)
      Mizoguchi T. Itoh M,
    • Journal Title

      生体の科学

      Volume: 66 Issue: 5 Pages: 440-441

    • DOI

      10.11477/mf.2425200297

    • ISSN
      0370-9531, 1883-5503
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Mindbomb 2 is dispensable for embryonic development and Notch signalling in zebrafish2015

    • Author(s)
      Mikami S, Nakaura M, Kawahara A, Mizoguchi T, Itoh M
    • Journal Title

      Biol Open

      Volume: 4 Issue: 11 Pages: 1576-82

    • DOI

      10.1242/bio.014225

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 9-Hydorxycanthin-6-one, a β-carboline alkaloid from Eurycoma longifolia, is the first Wnt signal inhibitor through activation of GSK3β without depending on CK1α2015

    • Author(s)
      Ohishi, K.; Toume, K.; Arai, M. A.; Koyano, T.; Kowithayakorn, T.; Mizoguchi, T.; Itoh, M.; Ishibashi, M.
    • Journal Title

      J. Nat. Prod.

      Volume: 11 Issue: 5 Pages: 1139-1146

    • DOI

      10.1021/acs.jnatprod.5b00153

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination2014

    • Author(s)
      Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.-I., Kawakami, K., and Itoh, M.
    • Journal Title

      Developmental Biology

      Volume: 391 Issue: 2 Pages: 196-206

    • DOI

      10.1016/j.ydbio.2014.04.011

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ricinine: a pyridone alkaloid from Ricinus communis that activates the Wnt signaling pathway through casein kinase 1α2014

    • Author(s)
      Kensuke Ohishi
    • Journal Title

      Bioorganic Medicinal Chemistry

      Volume: 22 Issue: 17 Pages: 4597-4601

    • DOI

      10.1016/j.bmc.2014.07.027

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Mib1 negatively regulates Ctnnd1 activity and controls cell migration.2015

    • Author(s)
      溝口 貴正 広瀬 和也 池田 祥子 渡邉 沙織 楊 薩薩 伊藤 素行
    • Organizer
      BMB2015(第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Notch Signaling Regulates V2 Interneuronal Differentiation and Neurite Outgrowth2015

    • Author(s)
      Miku Iihama, Takamasa Mizoguchi, Mizuki Nakaura, Michi Fukada, Miki Chin, Koichi Kawakami, Motoyuki Itoh
    • Organizer
      第21回小型魚類研究会
    • Place of Presentation
      大阪大学銀杏会館
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mind bomb2 is not essential for Notch signaling.2015

    • Author(s)
      Shohei Mikami, Mizuki Nakaura, Atsuo Kawahara, Takamasa Mizoguchi, Motoyuki Itoh
    • Organizer
      第21回小型魚類研究会
    • Place of Presentation
      大阪大学銀杏会館
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mib1 controls cell migration via negative regulation of Ctnnd1 activity.2015

    • Author(s)
      Takamasa Mizoguchi, Kazuya Hirose, Shoko Ikeda, Saori Watanabe, Sasa Yang, Motoyuki Itoh.
    • Organizer
      第48回日本発生生物学会大会(APDBN共催)
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] The actin dynamics live imaging reveals that Mib1 controls appropriate actin rearrangements and coordinates corrective cell migration.2015

    • Author(s)
      Takamasa Mizoguchi, Kazuya Hirose, Shoko Ikeda, Saori Watanabe, Sasa Yang, Motoyuki Itoh
    • Organizer
      Multi-dimensional fluorescence live imaging of cellular function and molecular activities
    • Place of Presentation
      Kyoto
    • Year and Date
      2015-01-26 – 2015-01-28
    • Related Report
      2014 Research-status Report
  • [Presentation] The appropriate regulation of the Notch signaling activity is required for various neuronal subtypedifferentiation and the neurite outgrowth of V2b interneuron2014

    • Author(s)
      Takamasa Mizoguchi, Mizuki Nakaura, Michi Fukada, Miki Chin, Koichi Kawakami, Motoyuki Itoh.
    • Organizer
      Notch研究会
    • Place of Presentation
      Osaka Unibversity
    • Year and Date
      2014-12-18
    • Related Report
      2014 Research-status Report
  • [Presentation] The appropriate control of the Notch signaling activity is required for various neuronal subtype differentiation and their functions2014

    • Author(s)
      Takamasa Mizoguchi, Mizuki Nakaura, Michi Fukada, Miki Chin, Koichi Kawakami, Motoyuki Itoh.
    • Organizer
      20th Japanese Medaka and Zebrafish Meeting
    • Place of Presentation
      Keio University
    • Year and Date
      2014-09-20 – 2014-09-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Mib1 regulates cell migration through negative control of Ctnnd1 function by its ubiqutination.2014

    • Author(s)
      Takamasa Mizoguchi, Kazuya Hirose, Shoko Ikeda, Saori Watanabe, Sasa Yang, Motoyuki Itoh.
    • Organizer
      11th International Conference on Zebrafish Development and Genetics
    • Place of Presentation
      Madison, Wisconsin, USA
    • Year and Date
      2014-06-24 – 2014-06-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a /V2b cell fate determination.2014

    • Author(s)
      Sayumi Okigawa, Takamasa Mizoguchi, Makoto Okanao, Haruna Tanaka, Miho Isoda, Yun-Jin Jiang, Maximiliano Susuter, Shinichi Higashijima, Koichi Kawakami, Motoyuki Itoh
    • Organizer
      47th Annual Meeting of the Japanese Society of Developmental Biologists
    • Place of Presentation
      Nagoya, Aichi
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Mib1 regulates cell migration via ubiquitination of p120ctn2013

    • Author(s)
      溝口 貴正
    • Organizer
      46th Annual Meeting for Japanese Society of Developmental Biology
    • Place of Presentation
      松江 くにびきメッセ
    • Related Report
      2013 Research-status Report
  • [Presentation] Mib1 controls cell migration through negative regulation of p120ctn function by its ubiquitination.2013

    • Author(s)
      溝口 貴正
    • Organizer
      The 19th Japanese Medaka and Zebrafish Meeting
    • Place of Presentation
      仙台 仙台市情報・産業プラザ
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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