The mechanism of ubiquitination system in collective cell migration.
Project/Area Number |
25840068
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Cell biology
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Research Institution | Chiba University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | Mib / p120ctn / Rac1 / 細胞集団移動 / ユビキチン化 / Mib1 / 細胞移動 / Mind bomb |
Outline of Final Research Achievements |
We found that the posterior lateral line primordium (pLLP) migration was delayed in mind bomb (mib) mutants. Mib is an E3 ubiquitin ligase and a positive regulator of Notch signal. We also found that Mib could potentially control cell migration via Notch signaling independent pathway. We showed that Mib interacted with and ubiquitinated p120 catenin (p120ctn), which controls cell migration. It is known that p120ctn overexpression induces activation of Rac1. We found that Mib suppressed p120ctn dependent Rac1 activation. These data indicate that Mib controls cell migration via negative regulation of p120ctn activity. Finally, we tested the hypothesis that exaggerated p120ctn activity in mib mutant causes the delay in pLLP migration. Morpholino knockdown of p120ctn rescued pLLP migration defects in mib mutants. Taken together, our data suggest that Mib is involved in pLLP migration through ubiquitination of p120ctn.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] 9-Hydorxycanthin-6-one, a β-carboline alkaloid from Eurycoma longifolia, is the first Wnt signal inhibitor through activation of GSK3β without depending on CK1α2015
Author(s)
Ohishi, K.; Toume, K.; Arai, M. A.; Koyano, T.; Kowithayakorn, T.; Mizoguchi, T.; Itoh, M.; Ishibashi, M.
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Journal Title
J. Nat. Prod.
Volume: 11
Issue: 5
Pages: 1139-1146
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination2014
Author(s)
Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.-I., Kawakami, K., and Itoh, M.
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Journal Title
Developmental Biology
Volume: 391
Issue: 2
Pages: 196-206
DOI
Related Report
Peer Reviewed
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[Presentation] Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a /V2b cell fate determination.2014
Author(s)
Sayumi Okigawa, Takamasa Mizoguchi, Makoto Okanao, Haruna Tanaka, Miho Isoda, Yun-Jin Jiang, Maximiliano Susuter, Shinichi Higashijima, Koichi Kawakami, Motoyuki Itoh
Organizer
47th Annual Meeting of the Japanese Society of Developmental Biologists
Place of Presentation
Nagoya, Aichi
Year and Date
2014-05-27 – 2014-05-30
Related Report
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