Analyzes of RNA-binding proteins involved in sexual differentiation of male germ cells and oogenesis in mice
| Project/Area Number |
25840091
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KATO Yuzuru 国立遺伝学研究所, 系統生物研究センター, 助教 (60570249)
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| Research Collaborator |
KATSUKI Takeo University of California, San Diego, Kavli Institute for Brain and Mind
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | マウス / 生殖細胞 / 性分化 / 卵形成 / Nanos2 / Dazl / 雄性分化 / RNA結合タンパク質 |
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| Outline of Final Research Achievements |
(1) We analyzed molecular function of Nanos2, and RNA-binding protein essential for sexual differentiation of mouse male germ cells. Particularly, we focused on an antagonistic interaction between Nanos2 and Dazl, a germ cell-speicific RNA-binding protein required for meiosis. We found that Nanos2 antagonized Dazl in sexually differentiating male germ cells and that Nanos2-mediated Dazl suppression plays an important role in the promotion of sexual differentiation of male germ cells.
(2) We addressed whether gene A plays roles in mouse oogenesis. We found that gene A was post-transcriptionally repressed in developing oocytes after birth. This gene A repression occurs in a 3'UTR-dependent manner. Furthermore, removing the 3'UTR in vivo stabilized gene A protein, resulting in the reduction of litter size. These results suggest that post-transcirptional repression of gene A is required for proper litter size in mice.
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Report
(3 results)
Research Products
(6 results)
