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Study on role of DNA methylation for plant cell reprogramming by using Arabidopsis epi-mutants.

Research Project

Project/Area Number 25840107
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagaoka University of Technology (2014)
Nagoya University (2013)

Principal Investigator

NISHIMURA Taisuke  長岡技術科学大学, 工学(系)研究科(研究院), 准教授 (10378581)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords細胞リプログラミング / DNAメチル化 / シロイヌナズナ / エピジェネティクス / 分化
Outline of Final Research Achievements

By the screening of Arabidopsis epiRILs (epigenetic Recombinant Imbred Lines) in which genetic sequences are mostly identical, but patterns of DNA methylation are highly variable on their genome, several lines that exhibit high or low efficiency of shoot regeneration from callus have been identified. In one of lines with high shoot regeneration efficiency, the phenotypes inherit stably with a dominant manner, and would be caused by at least two loci.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] BRU1 maintains configuration of the euchromatic subchromosomal domain in the nucleus of Arabidopsis.2014

    • Author(s)
      Ohno, Y, Nishimura, T., Hattori, T., and Takeda, S.
    • Journal Title

      Plant Mol. Biol. Rep.

      Volume: 32 Issue: 1 Pages: 19-27

    • DOI

      10.1007/s11105-013-0596-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] DNA methylation in gene silencing and cell reprogramming.2015

    • Author(s)
      Taisuke Nishimura
    • Organizer
      IPSR共同利用・共同拠点ワークショップ Plant Epigenetics: From Emerging Phenomena to Novel Molecular Events.
    • Place of Presentation
      倉敷市芸文館
    • Year and Date
      2015-01-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞リプログラミング過程に異常を示すシロイヌナズナ新規エピ変異体の探索2014

    • Author(s)
      西村泰介、山本章子、太田英惠、武田真、服部束穂。
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] The epigenetic factor acting downstream of DNA methylation in Arabidopsis.2014

    • Author(s)
      Taisuke Nishimura, Akiko Yamamoto, Larissa Broger, Katsushi Yamaguchi, Gregory Theiler, Etsuko Katoh, Shin Takeda, Tsukaho Hattori, Shuji Shigenobu, Jerzy Paszkowski.
    • Organizer
      The 38thNAITO CONFERENCE on Molecule-based biological systems
    • Place of Presentation
      CHATERAISE Gateaux Kingdom SAPPORO
    • Year and Date
      2014-10-07 – 2014-10-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞リプログラミング過程に異常を示す新規エピ変異体の探索2014

    • Author(s)
      西村泰介、山本章子、武田真、服部束穂。
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学 生田キャンパス
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脱分化・再分化過程に異常を示す新規エピ変異体2014

    • Author(s)
      西村泰介、山本章子、武田真、服部束穂。
    • Organizer
      第32回日本植物細胞分子生物学会大会
    • Place of Presentation
      アイーナ(いわて県民情報交流センター)
    • Year and Date
      2014-08-21 – 2014-08-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] カルスからの再分化過程に異常を示す新規エピ変異体の探索2014

    • Author(s)
      西村泰介、山本章子、武田真、服部束穂
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学 五福キャンパス
    • Related Report
      2013 Research-status Report
  • [Book] Nuclear Functions in Plant Transcription, Signaling and Development2015

    • Author(s)
      Yoko Ikeda, Taisuke Nishimura (一章分担執筆)、Olga Pontes, Hailing Jin (編集)
    • Total Pages
      182
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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