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Strategic isolation culture of ectosymbiotic methanotroph by engineering innovation

Research Project

Project/Area Number 25840166
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

WATSUJI Tomo-o  独立行政法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 研究員 (50409091)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords深海外部共生 / メタン酸化細菌 / メタン添加飼育 / 集積培養 / 純粋培養
Outline of Final Research Achievements

Shinkaia crosnieri in deep-sea hydrothermal vents in the Okinawa Trough has ingested epibiotic methanotrophs and thioautotrophs associated with setae in the ventral aspect. Therefore, we tried to rear S. crosnieri in an aquarium with added methane and succeeded in the rearing of S. crosnieri, which kept the association with epibiotic methanotrophs, during more than one year. We tried to isolate the epibiotic methanotrophs associated with S. crosnieri in the methane-added aquarium. But the epibiotic methanotrophs were not isolated in pure culture.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (1 results)

  • [Journal Article] Diversity and methane oxidation of active epibiotic methanotrophs on live Shinkaia crosnieri2014

    • Author(s)
      T. Watsuji, A. Yamamoto, Y. Takaki, K. Ueda, S. Kawagucci, and K. Takai
    • Journal Title

      The ISME journal

      Volume: 8 Issue: 5 Pages: 1020-1031

    • DOI

      10.1038/ismej.2013.226

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Allying with armored snails: the complete genome of gammaproteobacterial endosymbiont2014

    • Author(s)
      Satoshi Nakagawa
    • Journal Title

      The ISME Journal

      Volume: 8 Issue: 1 Pages: 40-51

    • DOI

      10.1038/ismej.2013.131

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Deep-sea Rhodococcus sp. BS-15 lacking the phytopathogenic fas genes, produces a novel glucotriose lipid biosurfactant.2014

    • Author(s)
      Konishi M., Nishi S., Fukuoka T., Kitamoto D., Watsuji T., Nagano Y., Yabuki A., Nakagawa S., Hatada Y. Horiuchi J.
    • Journal Title

      Mar Biotech

      Volume: 未定 Issue: 4 Pages: 484-493

    • DOI

      10.1007/s10126-014-9568-x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Post-drilling changes of fluid discharge and mineral deposition patterns and fluid chemistry in seafloor hydrothermal activity of the Iheya-North hydrothermal field, Okinawa Trough2013

    • Author(s)
      Kawagucci, S., Miyazaki, J., Nakajima, R., Nozaki, T., Takaya, Y., Kato, Y., Shibuya, T., Konno, U., Nakaguchi, Y., Hatada, K. Hirayama, H., Fujikura, K., Furushima, Y., Yamamoto, H., Watsuji, T., Ishibashi, J. and Takai, K.
    • Journal Title

      Geochemistry Geophysics Geosystem

      Volume: 14 Issue: 11 Pages: 4774-4790

    • DOI

      10.1002/2013gc004895

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.2013

    • Author(s)
      Mino, S., Makita, H., Toki, T., Miyazaki, J., Kato, S., Watanabe, H., Imachi, H., Watsuji, T., Nunoura, T., Kojima, S., Sawabe, T., Takai, K., and Nakagawa, S.
    • Journal Title

      Frontier in Extreme Microbiology

      Volume: 4 Pages: 107-107

    • DOI

      10.3389/fmicb.2013.00107

    • NAID

      120006389534

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 共生メタン酸化細菌を維持させてゴエモンコシオリエビを飼う2015

    • Author(s)
      元木香織、山本麻未、長井裕季子、羽田枝美、上田賢司、豊福高志、高井研、和辻智郎
    • Organizer
      ブルーアース2015
    • Place of Presentation
      東京海洋大学 品川キャンパス (東京都港区)
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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