Strategic isolation culture of ectosymbiotic methanotroph by engineering innovation
Project/Area Number |
25840166
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Ecology/Environment
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Research Institution | Japan Agency for Marine-Earth Science and Technology |
Principal Investigator |
WATSUJI Tomo-o 独立行政法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 研究員 (50409091)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 深海外部共生 / メタン酸化細菌 / メタン添加飼育 / 集積培養 / 純粋培養 |
Outline of Final Research Achievements |
Shinkaia crosnieri in deep-sea hydrothermal vents in the Okinawa Trough has ingested epibiotic methanotrophs and thioautotrophs associated with setae in the ventral aspect. Therefore, we tried to rear S. crosnieri in an aquarium with added methane and succeeded in the rearing of S. crosnieri, which kept the association with epibiotic methanotrophs, during more than one year. We tried to isolate the epibiotic methanotrophs associated with S. crosnieri in the methane-added aquarium. But the epibiotic methanotrophs were not isolated in pure culture.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Deep-sea Rhodococcus sp. BS-15, Lacking the Phytopathogenic fas Genes, Produces a Novel Glucotriose Lipid Biosurfactant2014
Author(s)
Konishi M., Nishi S., Fukuoka T., Kitamoto D., Watsuji T., Nagano Y., Yabuki A., Nakagawa S., Hatada Y. Horiuchi J.
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Journal Title
Marine Biotechnology
Volume: 未定
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Post‐drilling changes in fluid discharge pattern, mineral deposition, and fluid chemistry in the Iheya North hydrothermal field, Okinawa Trough2013
Author(s)
Kawagucci, S., Miyazaki, J., Nakajima, R., Nozaki, T., Takaya, Y., Kato, Y., Shibuya, T., Konno, U., Nakaguchi, Y., Hatada, K. Hirayama, H., Fujikura, K., Furushima, Y., Yamamoto, H., Watsuji, T., Ishibashi, J. and Takai, K.
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Journal Title
geochemistry geophysics geosystems
Volume: 14
Pages: 4774-4790
DOI
Related Report
Peer Reviewed
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[Journal Article] Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific2013
Author(s)
Mino, S., Makita, H., Toki, T., Miyazaki, J., Kato, S., Watanabe, H., Imachi, H., Watsuji, T., Nunoura, T., Kojima, S., Sawabe, T., Takai, K., and Nakagawa, S.
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Journal Title
frontiers in Microbiology
Volume: 4
Pages: 107-107
DOI
NAID
Related Report
Peer Reviewed
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