Strategic isolation culture of ectosymbiotic methanotroph by engineering innovation

• Project/Area Number: 25840166
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: WATSUJI Tomo-o 独立行政法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 研究員 (50409091)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 深海外部共生 / メタン酸化細菌 / メタン添加飼育 / 集積培養 / 純粋培養

Outline of Final Research Achievements

Shinkaia crosnieri in deep-sea hydrothermal vents in the Okinawa Trough has ingested epibiotic methanotrophs and thioautotrophs associated with setae in the ventral aspect. Therefore, we tried to rear S. crosnieri in an aquarium with added methane and succeeded in the rearing of S. crosnieri, which kept the association with epibiotic methanotrophs, during more than one year. We tried to isolate the epibiotic methanotrophs associated with S. crosnieri in the methane-added aquarium. But the epibiotic methanotrophs were not isolated in pure culture.

Research Products

• [Journal Article] Diversity and methane oxidation of active epibiotic methanotrophs on live Shinkaia crosnieri (2014)
  • Author(s): T. Watsuji, A. Yamamoto, Y. Takaki, K. Ueda, S. Kawagucci, and K. Takai
  • Journal Title: The ISME journal Volume: 8 Pages: 1020-1031
  • DOI: 10.1038/ismej.2013.226
  • Peer Reviewed
• [Journal Article] Allying with armored snails: the complete genome of gammaproteobacterial endosymbiont (2014)
  • Author(s): Satoshi Nakagawa
  • Journal Title: The ISME journal Volume: 8 Pages: 40-51
  • DOI: 10.1038/ismej.2013.131
  • Peer Reviewed
• [Journal Article] Deep-sea Rhodococcus sp. BS-15 lacking the phytopathogenic fas genes, produces a novel glucotriose lipid biosurfactant. (2014)
  • Author(s): Konishi M., Nishi S., Fukuoka T., Kitamoto D., Watsuji T., Nagano Y., Yabuki A., Nakagawa S., Hatada Y. Horiuchi J.
  • Journal Title: Marine Biotechnology Volume: 未定 Pages: 1-10
  • DOI: 10.1007/s10126-014-9568-x
  • Peer Reviewed
• [Journal Article] Post-drilling changes of fluid discharge and mineral deposition patterns and fluid chemistry in seafloor hydrothermal activity of the Iheya-North hydrothermal field, Okinawa Trough (2013)
  • Author(s): Kawagucci, S., Miyazaki, J., Nakajima, R., Nozaki, T., Takaya, Y., Kato, Y., Shibuya, T., Konno, U., Nakaguchi, Y., Hatada, K. Hirayama, H., Fujikura, K., Furushima, Y., Yamamoto, H., Watsuji, T., Ishibashi, J. and Takai, K.
  • Journal Title: geochemistry geophysics geosystems Volume: 14 Pages: 4774-4790
  • DOI: 10.1002/2013gc004895
  • Peer Reviewed
• [Journal Article] Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific. (2013)
  • Author(s): Mino, S., Makita, H., Toki, T., Miyazaki, J., Kato, S., Watanabe, H., Imachi, H., Watsuji, T., Nunoura, T., Kojima, S., Sawabe, T., Takai, K., and Nakagawa, S.
  • Journal Title: frontiers in Microbiology Volume: 4 Pages: 107-107
  • DOI: 10.3389/fmicb.2013.00107
  • NAID: 120006389534
  • Peer Reviewed
• [Presentation] 共生メタン酸化細菌を維持させてゴエモンコシオリエビを飼う (2015)
  • Author(s): 元木香織、山本麻未、長井裕季子、羽田枝美、上田賢司、豊福高志、高井研、和辻智郎
  • Organizer: ブルーアース2015
  • Place of Presentation: 東京海洋大学 品川キャンパス (東京都港区）
  • Year and Date: 2015-03-19 – 2015-03-20