Functional analysis of ROS signal regulator "RAM1" in plant defense
| Project/Area Number |
25850034
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MATSUI Hidenori 独立行政法人理化学研究所, 環境資源科学研究センター, 特別研究員 (20598833)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | MAMP / リン酸化タンパク質 / 細胞死 / 抵抗性 / R遺伝子 / 植物病理学 / MTI / 植物免疫 / リン酸化プロテオミクス / リン酸化 |
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| Outline of Final Research Achievements |
To identify novel plant immune components, we performed advanced phosphoproteomics approaches and identifed the mutant of “MAMP-responsive phosphoprotein for appropriated ROS kinatics 1”. MARK1 plays an important role for proper regulation of MAMP responses, but also functions as a regulator of cell death during R-gene mediated resistance in Arabidopsis thaliana. To reveal the molecular function of MARK1 protein, we performed yeast two hybrid assay to identify MARK1 interacting proteins (MIPs). We successfully isolated several MIPs, including mip1 which shows enhanced cell death phenotype, similar to mark1. These data suggest that MARK1-MIP1 interaction may regulate cell death during R-gene mediated resistance and this interaction likely play a role in plant immunity.
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Report
(3 results)
Research Products
(36 results)
