Enhancement of the fibrolytic consortia by the functional substances produced by rumen microbiota

• Project/Area Number: 25850061
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: SHINKAI TAKUMI 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門・家畜代謝栄養研究領域, 主任研究員 (70510254)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ルーメン / 細菌 / 繊維分解 / ルーメン発酵 / ルーメン細菌 / 遺伝子発現 / 網羅的遺伝子発現 / 代謝産物 / 微生物

Outline of Final Research Achievements

The present study tried to reveal the relationship among the community member of fibrolytic consortia mediated by functional substances produced by rumen microbiota. The metatranscriptomic assembly highlighted the characteristic fibrolytic enzymes, and indicated the importance of Fibrobacter succinogenes for the fiber digestion. Theβ-lactamase, indole compounds were found the possibility to affect the niche and fermentation products of fibrolytic consortium in the rumen.

Research Products

• [Journal Article] Comprehensive detection of bacterial carbohydrate-active enzyme coding genes expressed in the cow rumen (2016)
  • Author(s): Takumi Shinkai, Makoto Mitsumori, Ahmad Sofyan, Hiroyuki Kanamori, Harumi Sasaki, Yuichi Katayose, Akio Takenaka
  • Journal Title: Ａｎｉｍａｌ Ｓｃｉｅｎｃｅ Ｊｏｕｒｎａｌ Volume: 印刷中 Issue: 11 Pages: 1363-1370
  • DOI: 10.1111/asj.12585
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] メタボローム解析による粗飼料および濃厚飼料多給与時のルーメン発酵産物比較 (2016)
  • Author(s): 真貝拓三
  • Organizer: 第49回ルーメン研究会
  • Place of Presentation: 馬事畜産会館（東京都）
• [Presentation] オーキシン添加によるルーメン細菌およびルーメン発酵の変化 (2015)
  • Author(s): 真貝 拓三、三森 真琴
  • Organizer: 日本畜産学会
  • Place of Presentation: 宇都宮大学（栃木県・宇都宮市）
  • Year and Date: 2015-03-28
• [Presentation] Impact of the regulation of β-lactamase activity on fiber digestion and microbial composition in the rumen (2013)
  • Author(s): Shinkai T., Mitsumori, M., and Takenaka, A.
  • Organizer: China-Korea-Japan joint symposium on rumen metabolism and physiology
  • Place of Presentation: Changsha, China