Regulation of inflammation responses by HDL formation
Project/Area Number |
25850070
|
Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
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Research Institution | Kyoto University (2014) The University of Tokushima (2013) |
Principal Investigator |
NAGAO Kohjiro 京都大学, 工学(系)研究科(研究院), 助教 (40587325)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | コレステロール / ABCA1 / HDL |
Outline of Final Research Achievements |
ABCA1 mediates the efflux of cholesterol and phospholipids into apoA-I to form HDL, which is important in not only the prevention of atherosclerosis but also the regulation of inflammation responses. To develop a novel method for the evaluation of HDL formation, we prepared an apoA-I-POLARIC by labeling the apoA-I variant with a hydrophobicity-sensitive fluorescence probe that detects the environmental change around apoA-I during HDL formation. THP-1 macrophage-mediated HDL formation could be measured using apoA-I-POLARIC. These results demonstrate that HDL formation by ABCA1-expressing cells can be specifically detected by sensing hydrophobicity change in apoA-I, thus providing a novel method for assessing HDL formation.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] The roles of C-terminal helices of human apolipoprotein A-I in formation of high-density lipoprotein particles.2014
Author(s)
Nagao, K., Hata, M., Tanaka, K., Takechi, Y., Nguyen, D., Dhanasekaran, P., Lund-Katz, S., Phillips, M. C., and Saito, H.
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Journal Title
Biochim. Biophys. Acta-Mol. Cell Biol. Lipids
Volume: 1841
Issue: 1
Pages: 80-87
DOI
Related Report
Peer Reviewed
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[Journal Article] Interaction of Thioflavin T with Amyloid Fibrils of Apolipoprotein A-I N-terminal Fragment: Resonance Energy Transfer Study.2014
Author(s)
Girych, M., Gorbenko, G., Trusova, V., Adachi, E., Mizuguchi, C., Nagao, K., Kawashima, H., Akaji, K., Lund-Katz, S., Phillips, M. C., and Saito, H.
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Journal Title
J. Struct. Biol.
Volume: 185
Issue: 1
Pages: 116-124
DOI
Related Report
Peer Reviewed
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[Journal Article] The Extreme N-terminal Region of Human Apolipoprotein A-I Has a Strong Propensity to Form Amyloid Fibrils2014
Author(s)
Adachi, E., Kosaka, A., Tsuji, K., Mizuguchi, C., Kawashima, H., Shigenaga, A., Nagao, K., Akaji, K., Otaka, A., and Saito, H.
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Journal Title
FEBS Lett.
Volume: 588
Issue: 3
Pages: 389-394
DOI
Related Report
Peer Reviewed
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[Journal Article] ATPase activity of nucleotide binding domains of human MDR3 in the context of MDR12013
Author(s)
Ishigami, M.. Tominaga, Y., Nagao, K., Kimura, Y., Matsuo, M., Kioka, N. and Ueda, K
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Journal Title
Biochim Biophys Acta-MCBL
Volume: 1831
Issue: 4
Pages: 683-690
DOI
Related Report
Peer Reviewed
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