| Project/Area Number |
25850076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Sojo University |
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Principal Investigator |
HIRA Daisuke 崇城大学, 生物生命学部, 准教授 (00569890)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | anammox / ヘムタンパク質 / ヒドラジン合成 / 結晶構造解析 |
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| Outline of Final Research Achievements |
In this research, we attempted to elucidate the reaction mechanism of hydrazine synthesis system consisting of hydrazine synthase (HZS) and heterodimer cytochrome c (NaxLS) of anammox bacteria by the spectroscopic measurement and X-ray crystal structure analysis.
First, by site-specific mutagenesis to NaxLS, it was revealed that the reduction of NaxLS is essential for hydrazine synthesis and strongly suggested that the electron transfer from NaxLS to HZS is essential during the course of the reaction. Second, it was revealed that HZS has β-propeller like structure by the X-ray crystal structure analysis of HZS. Finally we revealed the crystallization conditions of HZS and NaxLS complex. We are now attempting the crystallographic analyses of the complex.
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