| Project/Area Number |
25850113
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | スギ / 花粉化石 / 葉緑体DNA / マイクロサテライトマーカー |
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| Outline of Final Research Achievements |
In this study, I tried to collect the fossil pollen grains of Cryptomeria japonica from the sediments of the marsh of the Tohoku region, and tried to amplify ancient DNA and compare the aDNA with DNA of present C. japonica populations. The genetic diversity of 17 chloroplast microsatellite markers was high enough to use for DNA analysis of fossil pollen grains. Furthermore, in two-isolated C. japonica populations adjacent to the marshes of Mt. Hakkoda, I identified the many trunks reproduced from a same individual by layering using the chloroplast microsatellite markers. However, DNA amplification was not observed from the sediments collected from the marsh. It was considered necessary to improve the method for DNA extraction from sediments.
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