Analysis of bovine-specific endogenous retrovirus sequences responsible for the placentation

• Project/Area Number: 25850183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tokyo University of Science (2014); Yokohama College of Pharmacy (2013)
• Principal Investigator: SAKURAI Toshihiro 東京理科大学, 薬学部, 助教 (70568253)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 内在性レトロウイルス / ウシ / 着床周辺期胚 / 胎盤形成 / 胎盤機能獲得 / 胚機能獲得

Outline of Final Research Achievements

Endogenous retrovirus (ERV) is thought to exist about 8-10% on the genome of placental mammals. Placentas in all mammals are known to provide similar functions, but the genes responsible for placental development differ among species, and remain largely unidentified. To identify ERV sequences unique to placentation of the bovine species, we characterized the expression profiles of bovine conceptus transcripts during the peri-attachment period using RNA-seq analysis. We isolated 10 bovine-specific ERV structures. We, further, evaluated the localization and the modification of gene expressions about 5 within the 10 ERV sequences. Besides, we improved the method for the evaluation of implantation in in vitro, and we analyzed the transcription factor responsible for the ERV expression. These observations support the argument that during the evolutionary process, mammalian species captured not only ERV sequences with similar structures but also sequences unique to individual species.

Research Products

• [Journal Article] Expression and in situ localization of GATA4, 5 and 6 mRNAs in ovine conceptuses and uterine endometria during the peri-implantation period. (2014)
  • Author(s): Bai H, Sakurai T, Godkin JD and Imakawa K.
  • Journal Title: Animal Science Journal Volume: 85 Issue: 4 Pages: 388-394
  • DOI: 10.1111/asj.12156
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Establishment and characterization of immortalized bovine endometrial epithelial cells. (2014)
  • Author(s): Bai H, Sakurai T, Bai R, Yamakoshi S, Aoki E, Kuse M, Okuda K and Imakawa K.
  • Journal Title: Animal Science Journal Volume: 85 Issue: 8 Pages: 799-804
  • DOI: 10.1111/asj.12202
  • NAID: 120006398763
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Invovement of VCAM-1 in the bovine conceptus adhesion to the uterine endometrium. (2014)
  • Author(s): Bai, bai, Kuse, Ideta, Aoyagi, Fujiwara, Okuda, Imakawa and Sakurai
  • Journal Title: Reproduction Volume: 147 Issue: 2 Pages: 119-127
  • DOI: 10.1530/rep-13-0655
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The localization of GATA2 in the nuclear and cytoplasmic regions of ovine conceptuses. (2014)
  • Author(s): Bai, Sakurai, Bai, Godkin and Imakawa
  • Journal Title: Animal Science Journal Volume: 85 Issue: 12 Pages: 981-985
  • DOI: 10.1111/asj.12267
  • NAID: 120006398762
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Down-regulation of interferon tau gene transcription with a transcription factor, EOMES (2013)
  • Author(s): Sakurai T, Bai H, Bai R, Sato D, Arai M, Okuda K, Ideta A, Aoyagi Y, Godkin JD, Imakawa K.
  • Journal Title: Molecular Reproduction and Development Volume: - Issue: 5 Pages: 371-383
  • DOI: 10.1002/mrd.22171
  • Peer Reviewed
• [Journal Article] Transcriptional Regulation of Two Conceptus Interferon Tau Genes Expressed in Japanese Black Cattle during Peri-Implantation Period. (2013)
  • Author(s): Sakurai T, Nakagawa S, Kim MS, Bai H, Bai R, Li J, Min KS, Ideta A, Aoyagi Y, Imakawa K.
  • Journal Title: PLoS One. Volume: 8 Issue: 11 Pages: e80427-e80427
  • DOI: 10.1371/journal.pone.0080427
  • Peer Reviewed
• [Presentation] ウシ不死化子宮上皮細胞の樹立 (2014)
  • Author(s): 関野貴洋，中嶌雄紀，山口美奈，箱田健匡，船谷桂子，小林芳子，加藤真介，櫻井敏博
  • Organizer: 日本薬学会 第134年会（熊本）
  • Place of Presentation: 熊本市総合体育館