| Project/Area Number |
25850183
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Tokyo University of Science (2014)
Yokohama College of Pharmacy (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 内在性レトロウイルス / ウシ / 着床周辺期胚 / 胎盤形成 / 胎盤機能獲得 / 胚機能獲得 |
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| Outline of Final Research Achievements |
Endogenous retrovirus (ERV) is thought to exist about 8-10% on the genome of placental mammals. Placentas in all mammals are known to provide similar functions, but the genes responsible for placental development differ among species, and remain largely unidentified. To identify ERV sequences unique to placentation of the bovine species, we characterized the expression profiles of bovine conceptus transcripts during the peri-attachment period using RNA-seq analysis. We isolated 10 bovine-specific ERV structures. We, further, evaluated the localization and the modification of gene expressions about 5 within the 10 ERV sequences. Besides, we improved the method for the evaluation of implantation in in vitro, and we analyzed the transcription factor responsible for the ERV expression. These observations support the argument that during the evolutionary process, mammalian species captured not only ERV sequences with similar structures but also sequences unique to individual species.
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