Development of a novel vaccine to control virulent strains of Marek's disease virus: enhancement of vaccine efficacy by modulating the host immunosuppression pathway

• Project/Area Number: 25850194
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: MURATA Shiro 北海道大学, (連合)獣医学研究科, 助教 (10579163)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: マレック病 / マレック病ウイルス / 組換えワクチン / PD1 / PD-L1 / 免疫抑制因子 / 感染性クローン / 組換えウイルス / PD-1

Outline of Final Research Achievements

Field isolates of Marek’s disease virus (MDV) have tended to increase in virulence, and therefore, the development of more effective vaccines would be desirable. To develop a novel vaccine, we focused on the PD1/PD-L1 pathway that contributes to the immune suppression, and reconstituted the recombinant MDVs that carry the soluble-PD1 gene to competitively inhibit the immunosuppression regulated by the endogenous PD1. The recombinant vaccine expressing soluble PD1 induced higher production of interferon γ in the immune cells from the vaccinated chickens, indicating that this vaccine potentially possesses an ability to induce more effective cell-mediated immunity in chickens.

Research Products

• [Journal Article] Expression of regulatory dendritic cell-related cytokines in cattle experimentally infected with Trypanosoma evansi. (2015)
  • Author(s): Mekata H, Murata S, Mingala CN, Ohashi K, Konnai S.
  • Journal Title: J Vet Med Sci. Volume: In press
  • NAID: 130005094682
  • Peer Reviewed
• [Journal Article] An investigation of binding ability of Ixodes persulcatus Schulze Salp15 with Lyme disease spirochetes (2015)
  • Author(s): Murase Y, Konnai S, Yamada S, Githaka WN, Isezaki M, Ito T, Takano A, Ando S, Kawabata H, Murata S, Ohashi K.
  • Journal Title: Insect Biochem Mol Biol. Volume: in press Pages: 59-67
  • DOI: 10.1016/j.ibmb.2015.01.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased expression of the regulatory T cell-associated marker CTLA-4 in bovine leukemia virus infection. (2015)
  • Author(s): Suzuki S, Konnai S, Okagawa T, Ikebuchi R, Nishimori A, Kohara J, Mingala CN, Murata S, Ohashi K
  • Journal Title: Veterinary immunology and immunopathology Volume: 163 Issue: 3-4 Pages: 115-124
  • DOI: 10.1016/j.vetimm.2014.10.006
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of PD-L1 on Canine Tumor Cells and Enhancement of IFN-γ Production from Tumor-Infiltrating Cells by PD-L1 Blockade (2014)
  • Author(s): Maekawa N, Konnai S, Ikebuchi R, Okagawa T, Adachi M, Takagi S, Kagawa Y, Nakajima C, Suzuki Y, Murata S, Ohashi K
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e98415-e98415
  • DOI: 10.1371/journal.pone.0098415
  • Peer Reviewed / Open Access
• [Journal Article] Identification and characterization of bovine programmed death-ligand 2 (2014)
  • Author(s): Nishimori A, Konnai S, Ikebuchi R, Okagawa T, Nakajima C, Suzuki Y, Mingala CN, Murata S, Ohashi K
  • Journal Title: Microbiol Immunol Volume: 58 Issue: 7 Pages: 388-397
  • DOI: 10.1111/1348-0421.12160
  • Peer Reviewed
• [Journal Article] Differences in cellular function and viral protein expression between IgMhigh and IgMlow B-cells in bovine leukemia virus-infected cattle (2014)
  • Author(s): Ikebuchi R, Konnai S, Okagawa T, Nishimori A, Nakahara A, Murata S, Ohashi K
  • Journal Title: J Gen Virol Volume: 95 Issue: 8 Pages: 1832-1842
  • DOI: 10.1099/vir.0.065011-0
  • Peer Reviewed
• [Journal Article] Identification and sequence characterization of novel Theileria genotypes from the waterbuck (Kobus defassa) in a Theileria parva-endemic area in Kenya (2014)
  • Author(s): Githaka N, Konnai S, Bishop R, Odongo D, Lekolool I, Kariuki E, Gakuya F, Kamau L, Isezaki M, Murata S, Ohashi K.
  • Journal Title: Vet. Parasitol. Volume: in press Issue: 3-4 Pages: 180-93
  • DOI: 10.1016/j.vetpar.2014.02.056
  • Peer Reviewed
• [Journal Article] 野生鳥類－畜産防疫の手引き~牛舎に侵入する野生鳥類. (2014)
  • Author(s): 長雄一, 大越安吾, 平井綱雄, 藤井啓, 大橋和彦, 村田史郎, 遠藤大二, 金子正美, 田中克佳, 浅川光彦.
  • Journal Title: 酪農ジャーナル Volume: 67 Pages: 29-31
• [Journal Article] Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek’s disease virus. (2014)
  • Author(s): Matsuyama-Kato, A., Murata, S., Isezaki, M., Takasaki, S., Kano, R., Konnai, S. and Ohashi, K.
  • Journal Title: Arch. Virol. Volume: In press
  • Peer Reviewed
• [Journal Article] Influence of PD-L1 cross-linking on cell death in PD-L1-expressing cell lines and bovine lymphocytes (2014)
  • Author(s): Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyma, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi.
  • Journal Title: Immunology Volume: (印刷中) Issue: 4 Pages: 551-61
  • DOI: 10.1111/imm.12243
  • Peer Reviewed
• [Journal Article] Suppressive effects of neutrophil by Salp16-like salivary gland proteins from Ixodes persulcatus Schulze tick (2014)
  • Author(s): Hidano A, Konnai S, Yamada S, Githaka N, Isezaki M, Higuchi H, Nagahata H, Ito T, Takano A, Ando S, Kawabata H, Murata S, Ohahsi K.
  • Journal Title: Insect Mol. Biol. Volume: in press Issue: 4 Pages: 466-74
  • DOI: 10.1111/imb.12101
  • Peer Reviewed
• [Journal Article] Characterization of Meq proteins from field isolates of Marek’s disease virus in Japan (2013)
  • Author(s): Murata, S., Hashiguchi, T., Hayashi, Y., Yamamoto, Y., Matsuyama-Kato, A., Takasaki, S., Isezaki, M., Onuma, M., Konnai, S., Ohashi, K.
  • Journal Title: Infect Genet Evol. Volume: 16C Pages: 137-143
  • DOI: 10.1016/j.meegid.2012.12.032
  • NAID: 120005317003
  • Peer Reviewed
• [Journal Article] Horizontal transmission of bovine leukemia virus from lymphocytotic cattle, and beneficial effects of insect vector control. (2013)
  • Author(s): Ooshiro M, Konnai S, Katagiri Y, Afuso M, Arakaki N, Tsuha O, Murata S, Ohashi K.
  • Journal Title: Vet Rec. Volume: 173 Issue: 21 Pages: 527-527
  • DOI: 10.1136/vr.101833
  • Peer Reviewed
• [Journal Article] Expression analysis of Focp3 in T-cells from bovine leukemia virus infected cattle. (2013)
  • Author(s): Suzuki S, Konnai S, Okagawa T, Ikebuchi R, Shirai T, Sunden Y, Mingala N. Claro, Onuma M, Murata S, Ohashi K
  • Journal Title: Microbiology and Immunology. Volume: 58 Issue: 8 Pages: 600-604
  • DOI: 10.1111/1348-0421.12073
  • Peer Reviewed
• [Journal Article] Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro (2013)
  • Author(s): Ryoyo Ikebuchi, Satoru Konnai, Tomohiro Okagawa, Kazumasa Yokoyma, Chie Nakajima, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi.
  • Journal Title: Veterinary Research Volume: 44 Issue: 1 Pages: 59-59
  • DOI: 10.1186/1297-9716-44-59
  • Peer Reviewed
• [Journal Article] Genotypic variations in field isolates of Theileria species infecting giraffes (Giraffa camelopardalis tippelskirchi and Giraffa camelopardalis reticulata) in Kenya (2013)
  • Author(s): Githaka N, Konnai S, Skilton R, Kariuki E, Kanduma E, Murata S, Ohashi K.
  • Journal Title: Parasitol Int. Volume: 62 Issue: 5 Pages: 448-453
  • DOI: 10.1016/j.parint.2013.06.002
  • Peer Reviewed
• [Journal Article] Bovine whole-blood culture as a tool for the measurement of endotoxin activities in Gram-negative bacterial vaccines (2013)
  • Author(s): Imamura S, Nakamizo M, Kawanishi M, Nakajima N, Yamamoto K, Uchiyama M, Hirano F, Nagai H, Kijima M, Ikebuchi R, Mekata H, Murata S, Konnai S, Ohashi K.
  • Journal Title: Vet Immunol Immunopathol. Volume: in press Issue: 1-2 Pages: 153-158
  • DOI: 10.1016/j.vetimm.2013.02.002
  • Peer Reviewed
• [Journal Article] Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus (2013)
  • Author(s): Konnai, S., Suzuki, S., Shirai, T., Ikebuchi, R., Okagawa, T., Sunden., Y, Mingala C.N., Onuma, M., Murata, S., Ohashi K.
  • Journal Title: Comp Immunol Microbiol Infect Dis. Volume: 36 Issue: 1 Pages: 63-9
  • DOI: 10.1016/j.cimid.2012.09.005
  • Peer Reviewed
• [Journal Article] Identification and partial characterization of a gut Rhipicephalus appendiculatus cystatin (2013)
  • Author(s): Imamura, S., Konnai, S., Yamada, S., Parizi, L.F., Githaka, N., Vaz, I. da S. Jr., Murata, S., Ohashi, K.
  • Journal Title: Ticks Tick Borne Dis. Volume: 4 Issue: 1-2 Pages: 138-44
  • DOI: 10.1016/j.ttbdis.2012.11.001
  • Peer Reviewed
• [Journal Article] Prevalence of Lyme Borrelia in Ixodes persulcatus Ticks from an Area with a Confirmed Case of Lyme Disease. (2013)
  • Author(s): MuraseY., Konnai, S., Githaka, N., Hidano, A., Taylor, K., Ito, T., Takano, A., Ando, S., Kawabata, H., Tsubota, T., Murata, S. and Ohashi, K.
  • Journal Title: J. Vet. Med Sci. Volume: 75 Pages: 538-540
  • NAID: 130001879630
  • Peer Reviewed
• [Journal Article] 免疫抑制受容体PD-1のリガンドPD-L2の機能的特徴と臨床応用研究 (2013)
  • Author(s): 西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 村田史郎, 大橋和彦
  • Journal Title: 動物用ワクチンーバイオ医薬品研究会ニュースレター Volume: 7 Pages: 19-20
• [Journal Article] 牛白血病ウイルスの感染予防対策 (2013)
  • Author(s): 今内覚, 村田史郎, 大橋和彦
  • Journal Title: DAIRYMAN Volume: 30 Pages: 60-62
• [Journal Article] Isolation, cloning and pathologic analysis of Trypanosoma evansi field isolates (2013)
  • Author(s): Mekata, H., Konnai, S., Mingala, C.N., Abes, N.S., Gutierrez, A., Dargantes, A.P., Witola, W.H., Inoue, N., Onuma, M., Murata, S., Ohash, K.
  • Journal Title: Parasitol Res. Volume: 112 Issue: 4 Pages: 1513-1521
  • DOI: 10.1007/s00436-013-3297-3
  • Peer Reviewed
• [Presentation] Increase of cells expressing PD-1 and PD-L1 in bovine leulemia virus infection and enhancement of anti-viral immune responses in vitro via its blockade. (2015)
  • Author(s): Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Asami Nishimori, Naoya Maekawa, Shiro Murata, Kazuhiko Ohashi.
  • Organizer: Immunity to Veterinary Pathogens: Informing Vaccine Development
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2015-01-20 – 2015-01-25
• [Presentation] Expression of PD-L1 on dog tumor cells and enhancement of IFN-g production from tumor-infiltrating cells by PD-L1 blockade. (2015)
  • Author(s): Naoya Maekawa, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Mami Adachi, Satoshi Takagi, Yumiko Kagawa, Shiro Murata, Kazuhiko Ohashi
  • Organizer: Immunity to Veterinary Pathogens: Informing Vaccine Development
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2015-01-20 – 2015-01-25
• [Presentation] Identification and characterization of bovine programmed death-ligand 2 (PD-L2). (2015)
  • Author(s): Asami Nishimori, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Chie Nakajima, Yasuhiko Suzuki, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
  • Organizer: Immunity to Veterinary Pathogens: Informing Vaccine Development
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2015-01-20 – 2015-01-25
• [Presentation] A novel strategy for immunotherapy of paratuberclosis in cattle: regulation of T-cell response to T-cell response to Mycobacterium avium subsp. paratuberclosis by the blockade of immunoinhibitory receptors. (2015)
  • Author(s): Tomohiro Okagawa, Satoru Konnai, Asami Nishimori, Seiko Mizorogi, Ryoyo Ikebuchi, Reiko Nagata, Satoko Kawaji, Shogo Tanaka, Yasuyuki Mori, Shiro Murata, Kazuhiko Ohashi
  • Organizer: Immunity to Veterinary Pathogens: Informing Vaccine Development
  • Place of Presentation: Keystone, Colorado, USA
  • Year and Date: 2015-01-20 – 2015-01-25
• [Presentation] Regulation of T-cell response to Mycobacterium avium subsp. paratuberclosis by immunoinhibitory receptors: a novel strategy for immunotherapy in cattle. (2014)
  • Author(s): Tomohiro Okagawa, Satoru Konnai, Asami Nishimori, Seiko Mizorogi, Ryoyo Ikebuchi, Shiro Murata, Kazuhiko Ohashi
  • Organizer: The 2nd Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Japan
  • Year and Date: 2014-09-24 – 2014-09-25
• [Presentation] Expression of PD-L1 on dog tumor cells and enhancement of IFN-g production by PD-L1 blockade. (2014)
  • Author(s): Naoya Maekawa, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Mami Adachi, Satoshi Takagi, Yumiko Kagawa, Shiro Murata, Kazuhiko Ohashi
  • Organizer: The 2nd Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Japan
  • Year and Date: 2014-09-24 – 2014-09-25
• [Presentation] Identification and characterization of bovine programmed death-ligand 2. (2014)
  • Author(s): Asami Nishimori, Satoru Konnai, Ryoyo Ikebuchi, Tomohiro Okagawa, Chie Nakajima, Yasuhiko Suzuki, Claro N. Mingala, Shiro Murata, Kazuhiko Ohashi
  • Organizer: The 2nd Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Japan
  • Year and Date: 2014-09-24 – 2014-09-25
• [Presentation] Molecular epidemiological survey of tick-borne infections of cattle in the Phillipines. (2014)
  • Author(s): Ochirkhuu Nyamusuren, Satoru Konnai, Claro N. Mingala, Tomohiro Okagawa, Shiro Murata, Kazuhiko Ohashi
  • Organizer: The 2nd Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Japan
  • Year and Date: 2014-09-24 – 2014-09-25
• [Presentation] Molecular epidemiological survey of vector-borne infections of cattle in the Phillipines. (2014)
  • Author(s): Ochirkhuu Nyamusuren, 今内覚, Claro N. Mingala, 岡川朋弘, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] ウシの免疫抑制受容体を介したヨーネ菌特異的T細胞応答の疲弊化. (2014)
  • Author(s): 岡川朋弘, 今内覚, 池渕良洋, 西森朝美, 溝呂木聖子, 永田礼子, 川治聡子, 田中省吾, 森康行, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] ウシ白血病ウイルス感染牛におけるナチュラル・キラー細胞の動態および機能解析 (2014)
  • Author(s): 中原綾子, 今内覚, 池渕良洋, 岡川朋弘, 西森朝美, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] ウシ制御生T細胞の機能解析および慢性感染症における動態解析. (2014)
  • Author(s): 大平浩輔, 今内覚, 岡川朋弘, 西森朝美, 前川直也, 中原綾子, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Direct PCR法を用いた全血からの牛白血病ウイルス迅速簡易診断法の検討. (2014)
  • Author(s): 西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 大平浩輔, 中原綾子, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] イヌ免疫抑制因子PD-1およびPD-L1を標的とした新規腫瘍免疫療法の検討. (2014)
  • Author(s): 前川直也, 今内覚, 池渕良洋, 岡川朋弘, 足立真実, 高木哲, 賀川由美子, 村田史郎, 大橋和彦
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Expression analysis of programmed death ligand 2 in tumors caused by the avian oncovirus Marek’s disease virus (2014)
  • Author(s): Shiro Murata, Ayumi Matuyama-Kato, Masayoshi Isezaki, Satoru Konnai, Kazuhiko Ohashi
  • Organizer: The 39th Annual International Herpesvirus Workshop.
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2014-07-19 – 2014-07-23
• [Presentation] Establishment of anti-bovine PD-1 monoclonal antibody and increase of anti-viral response by PD-1 blockade in bovine leukemia virus-infected cattle. (2013)
  • Author(s): Ikebuchi, R., Konnai, S., Okagawa, T., Yokoyama, K., Nakajima, C., Suzuki, Y., Murata, S. and Ohashi K.
  • Organizer: 10th International Veterinary Immunology Symposium
  • Place of Presentation: University of Milan, Italy
• [Presentation] Regulation of T cell exhaustion by blockade of PD-L1 and LAG-3 in persistently lymphocytotic cattle infected with bovine leukemia virus. (2013)
  • Author(s): Konnai, S., Ikebuchi, R., Okagawa, T., Murata, S. and Ohashi, K.
  • Organizer: 10th International Veterinary Immunology Symposium
  • Place of Presentation: University of Milan, Italy
• [Presentation] Regulatory dendritic cells play an important role in the control of trypanosoma evansi-induced massive inflammation. (2013)
  • Author(s): Mekata, H., Konnai, S., Mingala, C., Inoue, N., Murata, S. and Ohashi, K.
  • Organizer: 10th International Veterinary Immunology Symposium
  • Place of Presentation: University of Milan, Italy
• [Presentation] Increased expressions of immunoinhibitory receptor TIM-3 and its ligand during bovine leukemia virus infection. (2013)
  • Author(s): Okagawa, T., Konnai, S., Ikebuchi, R., Murata, S. and Ohashi, K.
  • Organizer: 10th International Veterinary Immunology Symposium
  • Place of Presentation: University of Milan, Italy
• [Presentation] Epidemiological research of Borrelia miyamotoi in Hokkaido. (2013)
  • Author(s): Yoshimura, H., Konnai, S., Toyomane, K., Takano, A., Kawabata, H., Murata, S. and Ohashi, K.
  • Organizer: The 1st Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Sapporo
• [Presentation] Upregulation of immunoinhibitory receptor Tim-3 leads to T-cell dysfunction in cattle infected with bovine leukemia virus. (2013)
  • Author(s): Okagawa, T., Konnai, S., Ikebuchi, R., Murata, S. and Ohahsi, K.
  • Organizer: The 1st Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Sapporo
• [Presentation] IgMhigh B cells are increase in number and prone to express viral antigen in bovine leukemia virus-infected cattle. (2013)
  • Author(s): Ikebuchi, R., Konnai, S., Okagawa, T., Nishimori, A., Nakahara, A., Murata, S. and Ohashi, K.
  • Organizer: The 1st Sapporo Summer Seminar for One Health
  • Place of Presentation: Hokkaido University, Sapporo
• [Presentation] 牛白血病発生農場におけるサシバエ種の同定とウイルスの検出 (2013)
  • Author(s): 今内覚, 豊間根耕地, 小池菜々子, 伊東拓也, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] シュルツェマダニ由来免疫抑制因子の機能解析 (2013)
  • Author(s): 豊間根耕地, 今内覚, 伊東拓也, 川端寛樹, 高野愛, 安藤秀二, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] イヌ免疫抑制因子PD-1およびPD-L1の同定と新規免疫療法樹立への基礎研究 (2013)
  • Author(s): 前川直也, 今内覚, 池渕良洋, 岡川朋弘, 高木哲, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] ウシ免疫抑制因子PD-L1およびPD-L2の組換えタンパク質作製と免疫機能解析 (2013)
  • Author(s): 西森朝美, 今内覚, 池渕良洋, 岡川朋弘, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] マレック病腫瘍病変形成における免疫抑制因子発現機構の検討 (2013)
  • Author(s): 松山あゆ美, 村田史郎, 伊勢崎政美, 今内覚, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] 全血培養法によるエンドトキシン活性測定の有用性の検討 (2013)
  • Author(s): 今村採貴, 中溝万里, 川西路子, 池渕良洋, 目堅博久, 村田史郎, 今内覚, 大橋和彦, 中島奈緒, 山本欣也, 内山万利小, 平野文哉, 永井英貴, 木島まゆみ
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] 牛白血病ウイルス感染牛由来IgMhighB細胞のウイルス抗原発現解析及び性状解析 (2013)
  • Author(s): 池渕良洋, 今内覚, 岡川朋弘, 西森朝美, 中原綾子, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Presentation] 国内に潜在すると考えられる新興回帰熱に関する疫学調査研究 (2013)
  • Author(s): 高野愛, 川端寛樹, 大久保（佐藤）梢, 中尾稔, 伊東拓也, Kyle Taylor, 李景利, 坪田敏男, 今内覚, 吉村英紘, 豊間根耕地, 村田史郎, 大橋和彦
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学、岐阜
• [Book] Recent viral disease of Japanese Anatid with a fatal case of Marek’s disease in an endangered species, white-fronted goose (Anser albifrons), In Ducks: Habitat, Behavior and Diseases (2013)
  • Author(s): Asakawa, M., Nakade, T., Murata, S., Ohashi, K., Osa, Y. and Taniyama, H.
  • Total Pages: 70
  • Publisher: Nova Science Publishers