| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The intracellular protozoan parasite Toxoplasma gondii is capable of invading any nucleated cell and replicates within a parasitophorous vacuole (PV). This microenvironment is modified by secretory proteins from organelles named rhoptries and dense granules. In this study, we identified the novel dense granule protein in Toxoplasma gondii. To understand the novel dense granule protein function, we performed disruption of this gene by homologous recombination, and analyzed its phenotypes.
The knock out strain showed similar growth rate as that of parental strain. The survival rate did not show significant difference between mice infected with knock out and parental strains. However, timing of egress was earlier than that of parental strain. These results suggested that this novel dense granule protein involved in regulating the parasite egress in T. gondii.
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