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Molecular Determinant Of Canine Distemper Virus To Modulate Usage Of The Human Receptors

Research Project

Project/Area Number 25850202
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

SAKAI KOUJI  国立感染症研究所, その他部局等, 主任研究官 (70515535)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsCDV / SLAM / nectin4 / ヒト / 宿主域 / 受容体 / イヌジステンパーウイルス / ウイルス / レセプター / 変異 / 感染症 / 獣医学 / 人獣共通感染症 / 実験動物学
Outline of Final Research Achievements

A canine distemper virus (CDV) strain, CYN07-dV, associated with a lethal outbreak in monkeys, used human signaling lymphocyte activation molecule as a receptor only poorly but readily adapted to use it following a R519S, D540G or P541S substitution in the hemagglutinin protein. Since CYN07-dV had an intrinsic ability to use human nectin-4, the adapted virus became able to use both human immune and epithelial cell receptors, as well as monkey and canine ones, suggesting that CDV can potentially infect humans. Unlike the CYN07-dV strain, these mutations did not confer hSLAM-using ability on the H proteins of wild-type CDV isolates. Our data demonstrated that a unique mutation of the CYN07-dV strain to adapt to humans and that the additional unique mutation in the H protein of wild-type CDV isolates is required to confer hSLAM-using ability.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Canine distemper virus associated with a lethal outbreak in monkeys can readily adapt to use human receptors.2013

    • Author(s)
      Sakai K, Yoshikawa T, Seki F, Fukushi S, Tahara M, Nagata N, Ami Y, Mizutani T, Kurane I, Yamaguchi R, Hasegawa H, Saijo M, Komase K, Morikawa S, Takeda M.
    • Journal Title

      J Virol

      Volume: 87 Issue: 12 Pages: 7170-7175

    • DOI

      10.1128/jvi.03479-12

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Host range and cell tropism determinants of measles virus and canine distemper virus2014

    • Author(s)
      Makoto Takeda, Kouji Sakai, Fumio Seki, and Noriyuki Otsuki
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      横浜、日本
    • Year and Date
      2014-11-10 – 2014-11-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 犬ジステンパーウイルスの霊長類レセプター利用に関する解析2014

    • Author(s)
      酒井宏治
    • Organizer
      Third Negative Strand Virus – Japan Symposium
    • Place of Presentation
      沖縄、日本
    • Related Report
      2013 Research-status Report
  • [Presentation] High potential of canine distemper virus in the ability to use macaca and human receptors2013

    • Author(s)
      Kouji SAKAI
    • Organizer
      15th International. Negative Strand Virus Meeting.
    • Place of Presentation
      Granada, Spain
    • Related Report
      2013 Research-status Report
  • [Presentation] サルから分離された犬ジステンパーウイルスのヒト受容体利用能獲得に必要な変異2013

    • Author(s)
      酒井宏治
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜、日本
    • Related Report
      2013 Research-status Report
  • [Presentation] 犬ジステンパーウイルスのヒトSLAM利用能獲得に必要な変異2013

    • Author(s)
      酒井宏治
    • Organizer
      第61回日本ウイルス学会学術集会
    • Place of Presentation
      神戸、日本
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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